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We compare it with the Votalin treating that have been proved effective on clinic during the experiment, and detect intraosseous pressure in the upper part of tibia and the content of nitrogen monoxidum in articular cartilages、 synovial membranes、 synovias of knee joint, we can initially identify the mechanism of action that this theropy treat on gonarthritis, supply the theoretical and experimental evidence for making general use of it on clinic.

以致骨内高压的方式造模,于兔腹股沟中点以下10mm处结扎并切断右侧股静脉建立兔膝骨关节炎模型,造模10周后各组采用相应的方法进行治疗,4周后将骨髓活检穿刺针穿刺达胫骨上端髓腔内,以三通管与传感器相连检测骨内压,后将动物处死,取膝关节软骨、滑膜、关节液,检测一氧化氮含量。

Hemarthrosis was found in the most of the joints in the early stage.

中后期(2w-3m),关节腔内可见关节渗出液,关节盘破坏、畸形以及髁突表面软骨破坏。

MRI can display not only in hyperosteogeny,but changing in articular meniscus,anticular cartilage,ligament and hydrarthrosis as well.MRI also shows instructive bearings in individualizing treatment and increases early diagnosis rate.

它是一种无创伤、无辐射、信息多的新方法,不仅能显示骨质病变,尤其能显示半月板、关节软骨、韧带及关节腔积液等病变情况,能提高早期诊断率,对治疗个体化有指导意义。

Results:MRI showed degeneration of meniscus in 16 joints,tearing in 7,changing of articular cartilage in 3,relaxing and thinning of lig.cruciatum anterius in 2 and lig.cruciatum posterius in 3,hydrarthrus in 6,changing synovial bursa and fibrous capsule each in 1.All these changes can not be seen in X-ray films.

结果:MRI显示半月板变性16个,撕裂7个;3个有关节软骨病变;前交叉韧带松弛变细2个,后交叉韧带病变3个;关节腔积液6个;滑囊、纤维囊病变各1个,而X线平片均无上述表现。

The results were expressed in mean±1SD. Pearson X~2 test and One-way ANOVA test were used. The data analyzed using the SPSS (version 11.5). Results: The sensitivity, specificity, positive and negative value of US for the LPEH model on the children cadaver were 88%, 84%, 79%, 91%, respectively. With regard to the thickness of femora head cartilage, the thickness of the anterior layer or posterior layer, there were no significant differences among three groups. However, the anterior layer was thicker than the posterior layer in three groups. The fluid in hip joint was detected in all of 21 symptomatic hips, which was clear commonly (90%) in early procedure. The amount of fluid in anterior recess showed a positive correlation with age (p .05). No fluid was detected in the asymptomatic and normal hips (2mm). The mean maximum width of inferomedial recess was significantly larger than that of anterior recess (12.50±4.04mm vs.4.35±0.8mm, p 0.05) in the symptomatic hip joints. The echogenic entrapped labral plicaes were demonstrated in the inferomedial recess in all of 21 children with LPEH, whose length and width ranged from 5.3mm-25.0mm (mean,15.6±5.6mm) and from 4.0mm-17.0mm (mean,8.9±7.8mm).

结果1,尸体LPEH髋关节模型的超声诊断敏感性、特异性分别为88%、84%,阳性预测值、阴性预测值分别为79%、91%。2,21例患儿的LPEH患髋(21侧)、健髋(21侧),以及21例正常儿童健髋(42侧)的超声检查显示:髋关节周围软组织及股骨头无形态结构差别;股骨头软骨厚度无统计学差异(3.5±0.5mm vs.3.6±0.4mm vs.3.6±0.5mm,p>0.05);关节囊前层及后层厚度无统计学差异(前层厚度2.79±0.74 mm vs.2.56±0.40mm vs.2.56±0.72mm;后层厚度2.70±0.82mm vs.2.48±0.54mm vs.2.44±0.58mm,p>0.05),但LPEH患髋关节囊前、后层均较后二组有增厚趋势。3,LPEH患髋均存在关节腔内积液,且早期较为清晰;积液以髋关节内下间隙明显,内下间隙较前间隙明显增宽(12.50±4.04mm vs.4.35±0.8mm,p<0.05),其内见嵌顿滑膜唇皱襞呈稍强回声的占位性团块,长约15.6±5.6mm,宽约8.9±7.8mm,90.5%(19/21)嵌顿皱襞内未见血流信号。4,所有LPEH患髋治疗后超声复诊均显示正常。

The major manifestations of MR imaging included synovial hyperplasia and pannus formation, joint effusion, bone marrow oedma, adnormality of signal and shape of meniscus, soft tissue swelling, cartilage destruction,popliteal lymphagogue, et al.

结果42例JIA患者的42个膝关节可见不同程度的MR异常改变(100%),主要影像学表现包括滑膜增生及血管翳形成、关节腔渗出积液、骨髓水肿、半月板形态及信号异常改变、关节软骨破坏、腘窝淋巴结肿大等。

Staining observation on complex mesochondrium of marrow-derived mesenchymal stem cells-TCP: It was found with TB staining that the complex of marrow-derived mesenchymal stem cells-TCP inside the joint cavity obviously had positive staining, which indicated that there were glycosidoprotein base formed.

骨髓基质干细胞-磷酸三钙复合体软骨基质特染观察结果:甲苯胺蓝染色发现,植入关节腔的骨髓基质干细胞-磷酸三钙复合体有明显阳性染色,表明有糖蛋白基质形成。

Conclusion] Somatostain intraarticular injection can relieve the inflammation and increase the SOD content of synovia of osteoarthritis.

结论]关节腔内注射生长抑素可使关节软骨损伤减轻,滑膜炎症得到控制,减轻关节液中SOD含量增加。

Histology showed that control group had massive cartilage cell gathering together and bone trabecula disturbance, it had no significant bone cortex and medullary cavity formation.

组织学检查显示:对照组中可见大量团聚软骨细胞、骨小梁紊乱,无明显骨皮质、髓腔形成。

2The histopathology of TMJ of PA rats showed pronounced proliferation of the synovium cells, some lytic collagenous fibers and degenerative chondrocytes in the condyles, and exudated protein and desquamating cells in the articular cavities with some fibrinous adhesions.

大鼠PA时,双侧TMJ均可见滑膜细胞显著增生;髁突表层胶元纤维松解,软骨细胞变性;关节腔可见蛋白渗出,细胞脱落,局部呈纤维性粘连。

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