软骨腔

Material and MethodsRA Rabbit Model group: 15 early RA rabbits of the same weight and variety Control group:10 normal rabbits of the same weight and varietyMethods of making RA rabbit model:elect 15 normal rabbits of the same weight and variety, dissolve Ovalbumin in 0.9% sodium chloride solution, to make the solution of 20mg/ml concengtration, blend the same quantity of complete Freund′s Adjuvant equably ,inject the mixed solution into endermic tissue of the rabbit′s scapular section, making the rabbits allergic, inject 1 ml of the mixed solution to one rabbit every time, inject 1 ml of the mixed solution in 5 different places of the rabbit′s scapular section, inject the rabbit one time every week,inject 3 weeks continuously,it turned out to be that the rabbits will be allergic, inject Ovalbumin blent with the 0.9% sodium chloride solution into the knee joint cavity of the rabbits in the fourth week, 5 mg Ovalbumin every knee joint cavity,two knees of every rabbit of the 15 rabbits are both injected,the arthrosis diameter and the exterior temperature of the knees will be added obviously in 24 hours,and they will drop gradually,at the time of the 14th or 21th day, the arthrosis diameter and the exterior temperature of the knees will get to the balance time,the incidence rate of RA is 100%.after the RA model succeeds,it is the early time of RA from the first week to the fourth week,after the fourth week,it is the late time of RA, the cartilage of the femoral condyle and the subchondral bone cortices will be changed unrecoverily.

资料与方法RA模型组：早期RA家兔模型15只，品种及体重接近。对照组：正常家兔10只，品种及体重接近。RA家兔模型制作方法：选取15只成年家兔，体重、品种接近，将卵蛋白溶解于生理盐水，配成浓度为20mg/ml的溶液，与等量完全弗氏佐剂混匀，注入家兔肩胛区皮下致敏，每只家兔每次注射1ml，于肩胛区5个不同区域注射，每周一次，连续注射3周而致敏，第4周向膝关节腔注射卵蛋白生理盐水溶液，每只关节腔注射5mg卵蛋白，15只家兔膝关节全部注射，24h内此关节直径和表面温度大幅度上升，以后缓慢下降，至14～21d达到平台期，发病率达100%。造模成功后第1～4周为早期改变，第4周以后出现不可逆的关节软骨及骨破坏。采用高频超声对RA模型组与对照组的膝关节髌上囊液体厚度、滑膜及股骨髁软骨厚度及软骨下骨皮质的回声情况进行对比观察。结果RA组模型组膝关节髌上囊积液及滑膜的厚度明显厚于对照组，其股骨髁软骨的厚度与对照组相比无明显差异，其软骨下骨皮质与对照组相比无明显改变。

From the commencement of week 3,the cartilage showed the characteristic of later OA morphologically with fibrosis of cartilage,sclerosis of sunchondral bone,amalgamation,opening and fibrosis of marrow cavity.

从第3周开始，正常的软骨细胞基本消失，呈纤维样变，软骨下骨硬化；髓腔融合、开放、纤维化，呈现晚期骨性关节炎的改变。

Objective To investigate the feasibility and the effectiveness of allograft chondrocytes mixed in the injectable thermosetting chitosan and glycerophosphate gel forming hyaline cartilage in vivo and repairing the whole layer defects of femur trochlea of rabbit keen joint.

目的探讨可注射性温固化凝胶壳聚糖-甘油磷酸钠复合同种异体软骨细胞在体内形成透明软骨和注入关节腔后修复关节软骨缺损的可行性和有效性。

Then the cultivated chondrocytes were embedded in fibrin glue fused on spongy bone, covered with priosteal flap; the complex was used to repair the femoral trochlea osteochondral defect which size is 3mm × 4mm × 4mm made in rabbit knee joint.

在A组的每只兔子的一侧膝关节股骨滑车部人为造成3mm×4mm的骨软骨缺损，骨刀切除软骨下骨到髓腔渗血为止(厚约4mm)，压迫后FG止血；取髂骨骨块，并尽可能保留松质骨，取下的骨块用PBS反复清洗，以除去血细胞，将松质骨填充在骨缺损处，松质骨面朝向关节腔，高度与周边软骨下骨齐平，把骨膜片生发层朝向关节腔，用无创伤缝合线缝合在周边的软骨或滑膜组织上；向EP管中加入1/2悬液体积的FG主体胶溶液并混匀，再与主体胶等体积的催化剂溶液一同注射入骨膜与骨块密闭的腔隙中；同理处理另一侧膝关节。B组处理与A组相比只是不加入软骨细胞；C组造成骨软骨缺损，FG覆盖创面后单纯用骨膜修复缺损。

Hemarthrosis and laceration of the disc and the attachment were also observed. 7 days later, the hemarthrosis was seldomly seen. Effusion was seen in some joints at 2 weeks. At 1 month posttrauma, the severely damaged structure of the two joints in one animal was found. We could find the loss of the disc and the cartilage of the condyle. Effusion also existed.

伤后1月，可见有一侧关节盘完全破坏，髁突软骨层消失，关节腔内渗出；对侧关节结构紊乱，关节盘及髁突明显变形、不规则等变化。3月后，多数关节结构基本正常，但关节内的破坏性改变仍然存在，关节内可见较明显的裂隙或囊状改变，软骨组织变薄，关节盘松解，关节腔扩张等变化。

Mm,humeroradial joint cavity 0.69±0.44 mm, proximal radioulnar joint cavity 0.90±0.56 mm, thethickness of joint cartilage (joint cartilage of humerus 1.15±0.35 mm, joint cartilage of ulna 1.04±0.16 mm, joint cartilage of radius 0.98±0. 17 mm) and the collateral ligament (thickness ofthe ulnar collateral ligament 1.38±0.42 mm, thickness of the radial collateral ligament 1.32±0.42 mm) were all measured.

结果：肱尺关节腔为0.70±0.44 mm，肱桡关节腔为0.69±0.44 mm，桡尺近侧关节腔为0.90±0.56 mm；关节软骨厚度，骨关节软骨厚度为1.15±0.35 mm，尺骨关节软骨厚度为1.04±0.16 mm，桡骨关节软骨厚度为0.98±0.17mm；侧副韧带：尺侧副韧带厚度为1.38±0.42mm，桡侧副韧带厚度为1.32±0.42mm。

In the MRI group, MR images were taken and assessed for location and severity of defects of cartilage by Noyes classification, osteophytes, subchondral cysts, sclerosis, meniscal and/or ligamentous tears.

MRI组根据MRI影像诊断软骨缺损的部位，对软骨缺损严重程度进行Noyes分级，对韧带、半月板、骨赘和软骨下骨囊腔改变情况进行评定，并诊断是否属于前内侧骨关节病变。

Results Extensive and diffuse synovial hyperplasia can be seen in patients with RA;synovial pannus formation can be seen in 25 cases.

结果RA组病人均可见广泛、弥漫的滑膜增生，其中25例病人可见滑膜血管翳形成，而OA组仅7例病人可见轻度滑膜增厚，未见滑膜血管翳形成者(X2=55.78P.005)；RA组关节软骨及软骨下骨改变较严重且弥漫，而OA组软骨退变则较局限，且以关节摩擦大的部位受损为著，其软骨下骨改变较为局限，常常见于IV软骨退变；关节腔积液、半月板及韧带改变，在OA组与RA组之间无差异。

Construct PLXRN-IL-1Ra and PLXRN-IL-10 vector, lapine synoviocytes were first transduced in culture by retroviral infection. The genetically modified synovial cells were then transplanted by intra-articular injection into the knee joints, assay of joint lavages confirmed that the gene expression was not lost after 14 days of transfer. Knees receiving the hIL-1Ra had significantly reduced cartilage breakdown. Delivery of the hIL-10 was less effective, When both genes were used together, there was a greater inhibition of cartilage breakdown and a considerable reduction of cartilage matrix degradation.

构建PLXRN-IL-1Ra、PLXRN-IL-10逆转录病毒载体，体外感染同种异体原代滑膜细胞，接着将转染了外源基因的滑膜细胞行关节腔注射入创伤性骨关节炎兔膝关节，通过对关节滑液的ELISA分析证明外源基因的表达在基因转移后14天还很稳定，只接受hIL-1Ra基因治疗的膝关节软骨损坏明显减轻，接受hIL-10基因治疗的膝关节治疗也有一定效果，当两种基因同时导入时，有非常明显的抑制软骨破坏和软骨基质降解的作用。

BMP/FG can induce cartilage regeneration and repair the full-thickness defects of cartilage with hyaline cartilage.

BMP/FG能在关节腔内诱导软骨再生，并能以透明软骨修复全层关节软骨缺损。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。