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Methods Thirty adult New Zealand rabbits were divided into three groups, group A,B,C, with ten rabbits in each group. One full-thickness defects of articular cartilage, 4 mm×5 mm×2.5 mm in size, was made in the trochlear groove of the left femur of each rabbit. The defects of group A and B were filled with BMP/FG and FG respectively. The defects of group C were left empty.

30只新西兰种成年兔随机分为A,B,C三组,每只兔子左膝股骨髁间凹做一大小为4 mm×5 mm×2.5 mm的全层关节软骨缺损。A、B组缺损内分别填充BMP/FG及FG,C组为空白。

INTERVENTIONS: A full-thickness defect in articular cartilage was made on the femoral trochlear surface by a drill of 5 mm in diameter and 3 mm in depth. Rabbits were allocated into filling group(type Ⅱ collagen sponge was grafted into left keen joint defect) and control group(right knee joint defect site was set as control) according to random number table.

干预:在股骨滑车面钻孔为直径5 mm、深3 mm的全层关节软骨缺损,按随机数分为填充组(左膝关节缺损部位植入Ⅱ型胶原海绵)和对照组(右膝关节缺损部位作为空白对照)。

Full-thickness cartilage defects, 0.5cm×0.5cm in size, were created at superior and lower area of femur trochlear in eight porcins (16 knees, 32 defects in all), the lesions in the right knees were untreated or treated by periosteum , the left knees were served as ACI groups (including monolayer and polylayer culture group), each defect was distributed into every control groups and every treated groups randomly.

在8头猪(16膝)股骨髁髌切迹上下各建立一个0.5cm×0.5cm大小的全层软骨缺损(共32个缺损),右膝为空白对照组和自体骨膜移植对照组,左膝为单层培养细胞组和复层培养细胞组,上下缺损随机进入各对照和试验组。

At 4,8,12 weeks after operation,the articular cartilages of tibial plateau were taken and the contents of uronic acidand hydroxyprolineand the water ratioin the cartilage were determined.

术后 4、 8、 1 2周取胫骨平台软骨做糖醛酸、羟脯氨酸含量及含水率的测定。

" Connective tissue: Tissue in the body that maintains the form of the body and its organs and provides cohesion and internal support, including bone, ligaments, tendons, cartilage, adipose tissue, and aponeuroses."

结缔组织:体内所有能起到躯体支撑、器官构架、组织粘合、细胞聚集作用的组织类型,如骨、韧带、腱、软骨、真皮和脂肪组织等。

As the collagen layer thickens, older collagen calcifies and older chondrocytes deteriorate.

随着胶原层变厚,原来的胶原钙和关节软骨细胞退化。

BACKGROUND: As an ideal scaffold of cartilage tissue engineering, demineralized bone matrix has been widespread used.

背景:脱钙松质骨被认为是一种&理想&的软骨组织工程支架材料已被广泛应用,但对其某些生物学特征目前仍不是十分清楚。

The bones were harvested, then bones length and length of their diaphyses were measured under stereo microscope. The histomorphometric analyses on midlongitudinal sections of bones were performed.

16d胎龄雌性小鼠尺骨在含有葛根素的BGJb培养基中旋转培养4 8h后,测量骨总长和骨干长度;HE染色,光镜下计数破骨细胞和肥大软骨细胞。

Results Histological investigation showed that the bone trabeculae were disarrayed containing dispersed cartilage cells in the denervated side, whereas the bone trabeculae disarryed orderly with rich blood vessels and no cartilage cell in the control side.

结果 实验侧牵张间隙骨小梁分布方向较杂乱,骨小梁内部有分散的软骨岛分布;对照侧牵张间隙骨小梁主要沿牵张方向分布,血管丰富,成骨细胞活跃。

Vertebral column were excided through dorsal meso-incision. the removed vertebral column were put on ice as soon as possible and L1/2, L2/3, L3/4, L4/5, L5/6 intervertebral disc tissue were procured.

取下整个腰椎段脊柱迅速放在冰面上,用眼科剪刀、镊剥离椎间小关节和椎体前方的软组织,分别从L1椎体下缘和L2椎体上缘分离上、下软骨终板,取出L1/2椎间盘组织。

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