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Relatively good results were also obtained similarly by using fresh autografts of periosteum and cartilage.

软骨细胞可以在筋膜上正常生长,筋膜可以作为软骨细胞移植的较为理想的载体。

RESULTS:①Retroviral vector EGFP-pLNCX2 transfected primary chondrocytes of rabbits,and high expression of transfected chondrocytes could be obtained through preliminary screening of G418.After being screened and expressing EGFP,chondrocytes kept normal morphology,with pseudopod adhering to the wall and matrix secreting vigorously.

结果:①重组逆转录病毒载体EGFP-pLNCX2转染原代兔软骨细胞,经G418初步筛选而获得的高表达格局,可见软骨细胞经过筛选和表达绿色荧光蛋白后,保持正常的形态学,细胞伸出伪足贴壁,基质分泌旺盛。

The rabbit model of osteoarthritis was established according to Hulth's method. The rabbits were sacrificed at the 8th week after administration of Yanghe Decoction for 14 days, and then rabbit tibia articular cartilage was removed. Sections of the cartilage were stained with Safranin O for histological examination. The cartilage histological characteristics were observed according to the method of Mankin. Immunohistochemical staining was performed to investigate the expression of VEGF.

按照Hulth法建立膝骨关节炎模型,术后第5周起至第7周末,阳和汤组予混有阳和汤的颗粒饲料,正常组及模型组均给予普通饲料;术后第8周分别取各组胫骨平台关节软骨,采用番红O染色,进行Mankin评分;免疫组织化学染色测定关节软骨中VEGF表达的阳性指数。

The central part was composed of hypertrophic chondrocytes, for which the number increased with time. The cartilaginous tissue at the time of 2 weeks' cultivation appeared strongly positive staining of collagen type Ⅱ immunohistochemistry and safranine "O", as well as strongly metachromatic to toluidine blue.

其中心为肥大软骨细胞,且随培养时间的延长而增多。2周时,软骨组织甲苯胺蓝染色呈强的红色异染反应,Ⅱ型胶原免疫组织化学染色呈强阳性,番红"O"染色呈强阳性。

Objective To explore an experimental method of transfecting the marrow stromal stem cells with the reconstructed PGL3-transforming growth factor-β1 (TGF-β1) gene and to evaluate the feasibility of self-induction of MSCs to the chondrocytes in vitro so as to provide a scientific and experimental basis for a further "gene enhanced tissue engineering" research.

目的 探讨将编码细胞转化生长因子β1(transforming growth factorβ1,TGF—β1)的重组PGL3-TGF-β1质粒转染兔骨髓基质干细胞(marrow stromal stemceils,MSCs),体外通过自分泌、诱导作用向软骨细胞方向分化的可行性,为基因加强组织工程学修复软骨损伤提供体外实验依据。

Damage in chondrocytes that ultimately causes them to senesce.

我们发现关节软骨中老化的软骨细胞随年龄的增加而累积。

This type of cancer begins in the cells that make cartilage, the stretchy tough material in joints.

软骨肉瘤,这类型的癌症开始在细胞,使软骨, stretchy强硬的材料在关节。

Osteoarthritis of the facet joints is similar to that of all diarthrodial joints, which displayed many changes such as cartilaginous surface defects, subchondral bone sclerosis, osteophyte formation, narrow capsule, apophyseal misalignment, and synovial hyperplasia.

腰小关节骨性关节炎同其他关节的骨性关节炎一样,表现为软骨面的病损,软骨下骨的硬化及骨赘形成,关节腔狭窄,关节囊增厚及滑膜增生等改变。

It can expedites proliferation and synthesizing active of cartilage cell and osteoblast too. Quicken ossification in the cartilagines and improving bone repair. But it has no influence to engendering blood vessel.

在骨愈合早期、中期,中药可加强巨核细胞的聚集及增强其活性,并可促进软骨细胞、成骨细胞的增殖及合成活性,加快软骨内骨化,促进骨愈合;而对血管形成无明显作用。

In order to repair chondral defects, autogenous chondrocyte transplantation is becoming encouraging treatment concerned by most investigators.

在软骨组织修复的治疗方法中,自体软骨细胞移植正成为目前研究和应用的重要方法。

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It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发,分析对称连杆曲线上鲍尔点的产生条件,提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品,年生产总值3000万美元,产品价廉物美、选料上乘、质量保证,深受国内外客户的青睐

The eˉtiology of hemospermia is complicate,but almost of hemospermia are benign.

血精的原因很,以良性病变为主。