软骨的

To study the effect of hTGF-β3 transfection into precartilaginous stem cells onproliferation and differentiation into cartilage chondrocytes.

探讨稳定表达重组TGF-β3对前软骨干细胞增殖及向成软骨方向定向分化的诱导作用。

This results in uneven distribution of stress transmission to deeper layers of cartilage and to the underlying subchondral bone.

这导致应力分布不均匀地传导至深层软骨及其下面的软骨下骨。

This results in uneven distribution of stre tra mi ion to deeper layers of cartilage and to the underlying subchondral bone.

这导致应力分布不均匀地传导至深层软骨及其下面的软骨下骨。

Chondrocytes from femur trochlear cartilage of eight porcins were isolated and cultured to 4th passage. These cells were counted and divided into 4 groups: monolayer culture group for 1w, polylayer culture group for 0w , 1w and 2w.

3.8头猪膝关节软骨标本经上述方法分离得到的软骨细胞传代培养第四代后，分别进入4个组别：单层培养1周组、复层培养0周组、1周组和2周组。

Autogenous or xenogenous osteochondral graft, cartilaginous membrane or periosteum graft and chondrocyte graft are commonly used grafts.

目前常用的移植物包括自体或异体骨软骨、软骨膜或骨膜及软骨细胞。

This deformity may be caused by collapse of the lateral crura, which is generally caused by overresection of cartilage and injury to its supporting structures, facial nerve palsy, unstable lower lateral cartilages, and vestibular stenosis.

这种畸形一般由因软骨过度切除，支撑结构损伤，面部神经麻痹，下外侧软骨不稳定和前庭狭窄造成的外侧脚塌陷引起。

Methods The total RNA was acquired from young rabbit particular cartilage, the BMP7 gene was inserted into pcDNA-3.1 to construct eukaryotic expression vector plasmid of pcDNA3.1-BMP7; the target gene-BMP7 was identified respectively by PCR analysis, restriction enounces analysis and nucleotide sequencing; the plasmid was transfected into adult rabbit knee articular chondrocytes, then the expression of BMP7 was detected by in situ hybridization, PCR and Western blotting.

从体外培养的幼兔膝关节软骨细胞中提取总RNA；按GenBank BMP7基因序列化学合成2条引物，采用RT-PCR方法得到BMP7基因；将BMP7基因片段插入到真核表达载体pcDNA 3.1中，构建pcDNA3.1-BMP7真核表达载体质粒；利用双酶切、PCR及核苷酸序列分析鉴定BMP7目的基因；将重组pcDNA3.1-BMP7真核表达载体质粒转染家兔关节软骨细胞，分别采用原位杂交、PCR、Western blotting法测定BMP-7表达。

Methods The operation was divided into two stages. In the first stage, the expander was implanted behind the ear, and was expanded. In the second stage, with autogenous costal cartilage or medpor materials, curve suitable framework, implant and suture it with remnant costal cartilage,overlap it by fascial flap behind the ear and expanded skin flap. Graft skin behind the ear.

手术分2期进行，Ⅰ期在耳后埋置扩张器并进行扩张；Ⅱ期手术以自体肋软骨或Medpor材料为支架，雕刻合适大小的支架，植入后与患侧耳残余软骨缝合，用耳后筋膜瓣和扩张皮肤覆盖支架，耳后区植皮。

Methods 65 cases（74 knee） of senile osteoarthritis of the knee were debrided under arthroscopy with local anesthesia.The procedures included removal of hyperplastic membrance and raw surface of degenerated cartilage,repairing the meniscus,and reshaping of the intercondylar fossa.

对65例（74膝）膝关节骨性关节炎患者采用局麻下行关节清理术，手术清理增生炎性滑膜，清理退变剥脱的软骨及打磨软骨创面，修整损伤半月板，取出关节内游离体、碎骨屑及炎性介质，髁间窝成形术解除撞击及磨损前交叉韧带。

The rabbit osteoarthritis model were duplicated by Hulth's technique. The pathologyical changes of each group was observed with naked eyes before taking the thighbone department carilage of pulley block and the platform place of shine bone carilage, then under the light microcope and the transmission electron microcope respectively, after treating with Semer Strychni (high、middle and low dose) for two months.

按Hulth法造兔膝骨关节炎模型，用马钱子高、中、低剂量进行灌胃治疗，连续2月后取材，进行肉眼大体观察后，取股骨滑车部软骨及胫骨平台处软骨，分别于HE染色后光镜下观察和铀-铅双重染色透射电镜下观察各组的病理变化。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。