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Results\ The proliferation of chondrocytes were very capable in the third to fourth generation and polyhedral angle\...

软骨细胞在体外培养 3周左右是作为软骨组织工程的最佳种子细胞

METHODS: In the experimental group, ADSCs were cultured with the scaffold and induced by CDMP1(50 μg/L) in basic medium for another 2 weeks. In the control group, SD rat cartilage cells were cultured with elementary nutrient liquid with scaffold for another 2 weeks. Twenty nude mice were imbedded the composite of cells-scaffold of the experimental group in their left armpits and the composites of cells-scaffold of the control group in right armpits. Every ten nude mice were killed at 8 and 12 weeks and were examined by safranine O-fast green and toluidine blue staining.

体外培养的脂肪干细胞复合于支架上,加入诱导液(基础培养液+ 50 μg/L软骨形态发生蛋白1)继续培养2周作为实验组;将SD仔鼠软骨细胞复合于支架上,常规培养2周作为对照组。20只裸小鼠,左侧腋窝皮下均植入实验组细胞-支架复合物,右侧腋窝皮下均植入对照组细胞-支架复合物。8, 12周各处死10只,行甲苯胺蓝和番红O-固绿染色。

At the 110th day,the semitransparent cartilage discs were 4.4 mm in diameter and 14.8mg in weightness.

结果 培养21天时形成"膜状软骨",至110天时形成直径约为4.4 mm的"圆盘状软骨"。

The defect was repaired with the osteochondral plug with the same diameter and depth taken from the proximal femoral trochlea using the OATS. The contralateral femoral trochlea was treated as normal control.

18 只新西兰兔右膝股骨滑车造成骨软骨缺损,取滑车临近部位相同直径和厚度的骨软骨柱修复缺损,左膝作为正常对照。

Sturgeons, are primitive chondrosteans fish. Although morphologically similar with shark, they were classified to Class Actinopterygii.

鲟 为古老的软骨硬鳞鱼类,其外形与鲨鱼相近,却是属於条鳍鱼纲、软骨硬鳞亚纲、鲟形目。

Second, the biphasic porous medium model is extended to describe dynamic behavior of articular cartilage.

其次,将关节软骨两相多孔介质模型推广到动力问题,得到描述关节软骨动力行为的力学模型。

Caudally the junction of the upper lateral cartilages with the cephalic edge of the lateral crus defines the scroll area.

上侧软骨尾侧端与外侧脚的头侧端相交处为软骨间交界区。

So that, we tried to find more effective method to diagnosis patellofemoral malalignment and chondral abnormalities.

关节镜下软骨成形术是治疗关节软骨退变的一种常用方法。

This experiment approach rat-MSCs which are fractional cultivation、generation in vitro, and in Chinese crude drug circumstance to derivationrat-MSCs chondroblast differentiated, to explore the front view ofcartilage coloboma architecture engineering recovery by Chinese crudedrug.

本实验探讨大鼠骨髓间充质干细胞在体外分离培养、增殖,以及在中药环境下诱导向成软骨细胞定向分化,探索软骨缺损组织工程修复中的中药应用前景。

OBJECTIVE: To investigate the chondrogenesis of bone marrow mesenchymal stem cells, chondrocytes and adipose-derived adult stromal cells in three-dimensional scaffolds

目的:考察3种种子骨髓间充质干细胞,软骨细胞和脂肪源性成熟基质细胞在支架中的成软骨特性。

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