软骨的

Abstract］ Objective To explore the diagnosis and clinical application values of MRI on chondromalacia patellae.Methods 12 cases of chondromalacia patellae were checked by orthovoltage X-ray,the sick knee joints were scanned with 0.2 T permanent magnetic MRI,and the displays of X-ray and MRI were analyzed.

目的 探讨MRI用于髌骨软骨软化的诊断及临床应用价值方法对12例髌骨软骨软化患者行常规X线检查，并使用0.2 T永磁型MRI对患病膝关节进行扫描检查，观察分析髌骨软骨软化的X线及MRI表现。

Hypertrophic chondrocytes, which are the terminally differentiated form of chondrocytes, play a key role in endochondral ossification.

肥大软骨细胞是软骨细胞的终末分化形式，在软骨内成骨过程中发挥十分关键的作用。

Results (1) Primary callus came into being 3 days after operation. Mesenchymal cells from peripheral tissues around the primary callus were differentiated into chondrocytes 1 week later. Endochondral ossification was going on then. Bridging callus was formed in the fracture sites 4 weeks after operation.

结果 (1)伤后3 d开始形成原始骨痂。1周时肉芽组织中的间质细胞开始分化为软骨细胞，软骨形成后再进行软骨内化骨。4周时形成连接骨折端的桥接骨痂。

Purpose To observe the difference of the different sequences and optimize scanning for articular cartilage.Materials and MethodsThree normal cadaver\' s wrists were examined with GE SIGNA EXITEⅡ3.0T MR scanner.SE T1WI、SE T2WI、PDWI、FS PDWIand 3D-FSPGR sequence were obtained with different flip and TE.

第一部分探讨3.0T磁共振腕关节软骨多序列成像的特点目的：观察不同扫描序列及参数对腕关节软骨成像质量的影响，优化获取3D-FSPGR(three dimension fat saturation spoiled gradient echo)序列最佳关节软骨扫描参数。

Greatest disc diameter was found for scaffolds seeded with auricular chondrocytes, followed by those with costal, nasoseptal, and articular cells.

最大的圆盘直径是来自于用耳软骨作为种子细胞构建的支架，其次是肋软骨，鼻中隔以及关节软骨细胞。

Results Positive staining for VEGF was distributed mostly in osteoblasts, osteoclasts and newly-born osteocytes. Vascular endothelial cells and bone marrow cells also showed VEGF positive reaction. Hypertrophic chondrocytes in calcifying region of chondroephysis were VEGF positive, but VEGF was absent from chondrocytes in other zones of chondroephysis.

结果 VEGF阳性反应主要见于成骨细胞、破骨细胞及新生骨细胞中，血管内皮细胞及骨髓细胞也呈阳性反应；骺软骨钙化区肥大的软骨细胞为VEGF阳性反应，但其它区域的软骨细胞中无VEGF表达。

Methods:(1) bFGF was loaded with PLGA, PELA and gelatin microspheres. The pharmac characteristics of particle size distribution, loading efficiency, entrapment efficiency, bFGF release kinetics were evaluated with scanning electronic microscope, MTT and ELISA respectively.

目的：制备bFGF缓释微球并作处方筛选，在体外培养的软骨细胞中观察其促有丝分裂作用和促基质合成作用，以及在兔膝关节全层软骨缺损模型中验证其促进软骨修复的作用，为其临床应用提供实验基础。

During the course of culture in vitro, the biological function of human nasal septal cartilage gradually degrade in accordance with increasing times of passage, chondrocytes after the third generation are not suitable for human cartilage tissue engineering seeds or studying cytobiology.

人鼻中隔软骨细胞在体外培养过程中，随传代次数的增加而功能活性逐渐降低，第3代以后的细胞不适于作为人软骨组织工程种子细胞和用于细胞生物学等方面研究关键词：葡萄糖氨基浆糖类；软骨细胞；细胞外基质；生物材料

Method］Isolated bMSCs were cultured in vitro. rh Fibroblast Growth Factor 1(rhFGF-1)、rh Transforming Growth Factor-β1(rhTGF-β1)、rh Insulinlike Growth Factor-Ⅰ were utilizated. The proliferation of cells was detected by MTT assay, and the macroscopic histology , HE staining and immunohistochemical examinations were performed to seek the best cell factor. In vivo , to investigate the repair of the articular cartilage, bMSCs combined with fibrin glue and rhTGF-β1、rhIGF-I was compared with control group.

方法］rhFGF1、rhTGF-β1、rhIGF-I单独或联合应用对骨髓基质干细胞进行体外诱导培养，应用常规染色、MTT、免疫组织化学染色的方法筛选诱导骨髓基质干细胞成软骨细胞的最佳细胞因子，并将其与骨髓基质干细胞复合于纤维蛋白凝胶制成凝胶复合物，直接种植到兔膝关节实验性关节软骨缺损处，并与对照组相比较，观察软骨修复效果。

Methods The chondrocytes isolated from mini swines' ears were mixed with injectable biocompatible matrix,and the density of cell suspensions were 10,20,30,40,50,60,70×10 6/ml.

结果在动物自体内形成的软骨组织，与正常软骨组织有相似的组织学特性，形成软骨组织最适细胞浓度为50×106／ml，最佳形成时间是第6周。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。