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The large defects at articular cartilage are often repaired by fibrocartilage rather than normal hyaline cartilage .

大的关节软骨缺损常由纤维软骨而不是正常的透明软骨修复。

Results The tissue engineered tissue from 1 week group is more like fibrocartilage. From 2 week group,the tissue gradually transfer into hyaline cartilage and form more mature hyaline like cartilage in 4 weeks group.

结果:经组织学染色发现:在诱导的初期组织更偏向于纤维软骨,而从第二周开始组织逐渐向透明软骨转化,至第4周逐渐形成成熟的透明软骨样组织。

Nowadays, it was known that auricular cartilage can roughly be separated into three layers: fibrous, cambium and cartilage layer. The fibrous layer is at the outer part of perichondrium, and the cambium layer is the inner part.

目前已知在软骨组织大致可分三层细胞,包括最外侧的纤维层、软骨组织及在软骨组织表层的形成层。

To investigate the stantant changes of Ⅰ、Ⅱ、Ⅲ and PG expression of mandibular condylar chondrocytes stimulated by the different static compressive stresses, so that to make a primary understanding of the relationship between the differentiation and dedifferentiation of condylar chondrocytes and different compressive stresses; to investigate the stantant changes of the content of DNA of mandibular condylar chondrocytes stimulated by the different static compressive stresses, so that to make a primary understanding of the relationship between proliferentiation and apothesis of MCCs and different static compressive stresses.

观察单层培养的髁状突软骨细胞加载不同静压力后,Ⅰ、Ⅱ、Ⅲ型胶原以及蛋白多糖表达的变化,探讨不同静压力对髁状突软骨细胞的分化及去分化的即时效应;观察单层培养的髁状突软骨细胞加载不同静压力后,其增殖、凋亡及细胞周期的变化,探讨不同静压力对髁状突软骨细胞增殖及凋亡的即时效应。

MRI of 36 cases with acute cartilage injury of knee confirmed by arthroscopy and arthrostomy were retrospectively analyzed, with emphasis on articular cartilage and subchondral lesion.

分析其中36例经关节镜或手术证实关节软骨损伤患者的膝关节MRI所见,着重观察关节软骨和软骨下骨的改变,并与关节镜和手术结果对照分析。

Mri of 36 cases with acute cartilage injury of knee confirmed by arthroscopy and arthrostomy were retrospectively analyzed, with emphasis on articular cartilage and subchondral lesion.

分析其中36例经关节镜或手术证实为关节软骨损伤患者的膝关节mri所见,着重观察关节软骨和软骨下骨的改变,并与关节镜和手术结果对照分析。

The method is availabe to solve the problem which chondrocytes were easy to lost when they were used to repair the defects of articular cartilage in clinic. There are no reports about this kind of method of autogenic transplantation.

这种应用以自体关节软骨细胞制备的组织工程软骨移植物修复自体关节软骨组织缺损的方法尚未见有文献报道。

In summary, hyaluronic acid and autologous synovial fluid induces chondrogenesis of equine mesenchymal stem cells, which encourage tissue engineering applications of MSC in chondral defects, as the natural environment in the joint is favorable for chondrogenic differentiation.

总之,透明质酸和自体关节液诱导马的间充质干细胞的软骨形成,这鼓励组织工程在软骨缺损修复中应用间充质干细胞,因为关节自然环境是有利于软骨形成分化。

There are different viewpoints about the live of costal cartilage autograt, the repair of offer region and the recovery style of the cartilage, especially about the selection of surgical time in congenital microtia, the use of chondrium in the growth of cartilage autograft and the growth ability of the reconstructed ear in preschool children.

对于自体肋软骨移植后能否成活,肋软骨膜在移植软骨中的作用,供区的修复以及软骨间的愈合方式等均存在不同的看法;特别是对先天性小耳畸形患儿的手术时机的选择,肋软骨膜在移植软骨生长中的作用以及学龄前儿童再造耳能否随年龄增长而与正常耳同步按比例生长等均存在不同的观点。

RESULTS: During 10 - 12 weeks, in cuntrol group: The defect area was repaired by white and soft tissue that had no resistance to press. The repaired tissue was still lower than the surrounding articular surface with clear boundary. By histological observation, it was found that the defect was repaired by the mechanism similar to inflammatory reaction and the defect is ultimately filled by the hyperplasia of hyaline degenerative fibrous tissues. In filling group: the defect was repaired by semi-transparent, smooth, textured tissues with polish that had resistance to press as well as elasticity. The repaired tissue was almost similar to the shape of the surrounding cartilage,difficult to be distinguished. After histological observation, it was found that there was no inflammatory reaction, but active hyperplasia of inner bonetissue and cartilage tissues; a lot of osteoid tissues and trabeculation were found. Newlborn cartilage was fused with surrounding cartilage tissue and connected with surrounding tissues.

结果:10~12周,对照组:缺损区由白色、质软、按压无阻抗的组织修复,修复组织仍低于周围关节面,边界仍清晰可辨,组织学以类似炎症反应的机制修复缺损,最终以透明变性的纤维组织的增生来填补缺损部位;填充组:缺损区由半透明状、质韧光滑有光泽,按压有阻抗并有弹性的组织修复,修复组织与周围软骨外形上已基本相似,不易区分,组织学未见有炎症反应的过程,内骨组织和软骨组织增生活跃,并可见大量类骨组织和骨小梁形成,新生软骨和周围软骨组织融合,并与周围组织连接。

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