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Six weeks after implantation, small amount of oval lacunae appeared. PGA fibers began to be absorbed. The amount of collagen increased and Safranin O stained positively. Eight weeks after implantation, cartilage with single or occasionally double chondrocytes in round lacunae was found.

体内埋植4周后可见PGA纤维间被不成熟的软骨细胞充填。6周时出现椭圆形软骨陷窝,PGA纤维开始吸收,胶原增多,番红花—"O"有阳性染色。8周时软骨陷窝呈圆形,单个,双个对称或多个排列。

A new parallel-epiphyseal cartilaginous tissue was found in the subcutaneous tissue 4 weeks after operation. The tissue was stained with HE, toluidine blue, safranine O and collagenⅡ. A large number of chondrocytes and a cartilage lacuna-like structure were observed under a microscope with no obvious inflammatory reaction around the calcium alginate and polylysine microspheres.

结果 体外培养时微球中骺板细胞生长、增殖情况良好;移植皮下后第4周取材可见外形呈类骺软骨样组织块,行HE、甲苯胺蓝、番红"O"及Ⅱ型胶原等染色,镜下观察可见大量软骨细胞及类软骨陷窝样结构,植入的海藻酸钙微球周围组织无明显炎症反应。

Objective To investigate the feasibility and the effectiveness of allograft chondrocytes mixed in the injectable thermosetting chitosan and glycerophosphate gel forming hyaline cartilage in vivo and repairing the whole layer defects of femur trochlea of rabbit keen joint.

目的探讨可注射性温固化凝胶壳聚糖-甘油磷酸钠复合同种异体软骨细胞在体内形成透明软骨和注入关节腔后修复关节软骨缺损的可行性和有效性。

Of, relating to, or being a wedge-shaped bone or cartilage .

软骨,肉中软骨软骨,尤指在肉中出现的

But the chondrocytes lost primary characteristics after several passages.Conclusion A great of chondrocytes can be got from xiphoid, and the chondrocytes can grow in suspension and keep primary characteristics for a long time when they are cultured with polyHEMA in vitro. In this way, good seed cells are provided for experiment in vitro, and for the study of tissue engineering, and for clinical study.

从剑突可获得大量软骨细胞,使用PolyHEMA试剂体外培养的软骨细胞,其软骨细胞能长期以悬浮状态生长,并能使软骨细胞长期保持原有的生物学特性,为体外实验及组织工程学的研究提供更优质种子细胞,为临床的进一步研究提供理论基础。

In the myopic eyes the cartilaginous sclera was thicker relatively, and the ratio of binucleate cells/chondrocytes was higher than that in control eyes.

形觉剥夺使巩膜软骨层的相对厚度增加,双核软骨细胞百分数增加,实验眼软骨层和纤维层边界欠清,去遮盖后恢复正常。

Articular chondrocytes from the knee joints of the A group were separated respectively to fragments, and then digested by enzymes, both the chondrocytes and the uncomplite digested tiny chondral tissues have been cultured in vitro for about 2 weeks.

分别取A组9只兔子的双侧膝关节股骨滑车及部分髁部的软骨,在体外进行分离和消化,未完全消化的软骨组织加软骨细胞在培养瓶内一同培养2周后配制成浓度为1.6×10~8/ml的软骨细胞悬液,等分装于两个EP管中。

Methods: Based on part one, implanted the chondroblast of second generation on porous materials, the cells were distributed randomly into three groups, A was PDLLA group, B was PDLLA/chitosan group, C was chondroblast groups.

利用第一部分经成软骨诱导、并经鉴定具有成软骨细胞表型的第一代细胞,分别种植于PDLLA多孔材料表面、PDLLA/壳聚糖多孔材料表面,分PDLLA材料组、PDLLA/壳聚糖材料组、诱导成软骨细胞单层培养组。

OBJECTIVE: To explore the feasibility of in vivo chondrogenesis by co-culture of bone marrow-derived MSCs and chondrocytes.

背景:诱导因子及软骨微环境是影响骨髓基质干细胞成软骨分化及软骨形成的主要因素。

The model had good duplicability and stability through the method of establishing the animal model in which the simple operative method,small wound and convenient nurse was obtained so easilyis that the animal model was spread easily.The study of the change of short and long period was made to provide the experimental base and reason through the animal model.

结论通过自制冲击器冲击兔右侧髋臼软骨,模拟冲击伤机制,初步建立髋臼软骨冲击伤实验动物模型并量化冲击能,通过统计学分析,大体和组织病理学检查,并与国外动物模型相比,本模型重复性和稳定性好,术式操作简单,创伤小,护理方便,易于推广,为研究活体动物髋臼软骨冲击后的短期和长期变化提供实验基础和依据。

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