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Cartilage damage can also occur as part of other rare conditions such as osteochondritis dissecans (where loss of blood supply causes a piece of bone and cartilage to separate from the bone) and relapsing polychondritis (which causes swelling of the cartilage in the ear).

软骨损伤,也可能会出现的一部分,其他罕见的条件,如剥脱性骨软骨炎(其中损失的血液供应的原因一块骨与软骨脱离骨)和复发性多软骨炎(造成肿胀,关节软骨在耳)。

Vascular endothelial growth factor is an important angiogenine. There is accumulating evidence shows that expression of VEGF in mature chondrocyte can promote cell redifferentiation and caryocinesia activity. VEGF plays a key role for chondrocyte to survival and proliferation during the chondrogenesis.

对成熟软骨细胞分泌表达蛋白的研究发现,软骨细胞表达一定水平的VEGF以提高软骨细胞有丝分裂活性并促进其再分化,在软骨发生发育过程中,对软骨细胞的存活与增殖起着重要的作用。

Methods Use the OS specimen of 57 cases of amputation,saw these specimen into spicula of 1cm thick in vertical direction,and make X-ray photograph of multi-spicula,Draw materials in various plane of epiphyseal plate and cartilage ,observe the invasive condition of OS to epiphyseal plate and cartilage under light microscope.

将38例截肢的OS标本,纵向锯成1cm左右的骨片,作多骨片X-线照片,在不同层面骨骺软骨板和关节软骨处多处取材,光学显微镜观察OS对骨骺软骨板和关节软骨侵犯情况。

At 12 week, the cartilage surface of the MSCs/ HA/CPP/PLLA group was smooth, cartilage layer with normal thickness and complete subcartilaginous bone were observed with light microscope, which had significant differences compared with the control group.

细胞材料复合物组12周关节软骨表面光滑,光镜下可见已形成正常软骨厚度的软骨层及完整的软骨下骨,与对照组有明显差异。

Results Same as hyaline cartilage, the calcified cartilage was stained light flavo-green and weak double refraction by the Picrosirius-polarization method. In the immunohistochemistry analysis of collagen Ⅱ protein, the calcified cartilage was stained strong green fluorescence and the collagen protein parallelled in joint surface near the tidemark. The content of collagen Ⅱ protein was (20.16%±0.96%) of tissue dry weight by amino acid analysis, and that of the hydroxyapatite was (65.09%±2.31%) of the inorganic composition in calcified cartilage.

结果 天狼猩红染色见钙化软骨层与透明软骨层一样黄绿色淡染、弱双折光性;Ⅱ型胶原蛋白免疫组织化学分析,钙化软骨层呈强绿色荧光,潮线附近的胶原蛋白呈板层平行分布;氨基酸定量分析钙化软骨层Ⅱ型胶原蛋白含量占组织干质量的(20.16%〖KG-*4〗±0.96%);X-射线衍射定量分析钙化软骨层中的羟基磷灰石含量占无机成分的(65.09%±2.31%)。

The chondroblast cultured with compound porous materials PDLLA and PDLLA/chitosan. The aim are: 1 To examine the proliferation ability and potential chondroblast differentiation; 2 To find an ideal condition stimulated BMSC differentiate into chondroblast; 3 To examine the chondroblast proliferation in porous scaffolds and to explore the interaction between cells and materials; 4 To examine the release of cytokine in vitro.

目的在于:(1)通过骨髓基质干细胞的原代分离、培养及成软骨诱导,观察原代细胞扩增能力及其向成软骨方向分化的潜能;(2)寻求骨髓基质干细胞向成软骨方向分化的理想诱导条件;(3)观察经成软骨诱导的MSC在PDLLA、PDLLA/壳聚糖多孔载体上三维的增殖状况,探求细胞与材料间的相互作用;(4)观察经成软骨诱导的MSC在体外三维培养系统中细胞因子的释放状况,了解维持诱导细胞软骨表型的合适条件及作用机制。

Recently, bipolar radiofrequency energy has become popular as a method of performing chondroplasty because of accurately treatment to degenerative cartilage and minimal injury to normal cartilage. But the questions of the effects of bipolar RFE to degenerative cartilage, the reaction of chondrocyte and cartilage matrix, the change of the cartilage after the treatment, and the influence to the cartilage degenerative course, were still unknown.

但是双极射频在关节软骨退变治疗中究竟发挥何种作用,治疗区软骨细胞及软骨基质成分反应是怎样的,随着时间的推移软骨表面的变化如何,双极射频对软骨退变的病程有无影响,这些问题都不清楚。

The process of osteoarthritis mesochondrium degradation is possibly attributed to the synergistical consequence of MMPs and other factors. Pilose antler peptide can promote the proliferation of OA chondrocytes of rabbits ,inhibit the overexpression of MMPs of OA chondrocytes.It can possibly play a effective role in the treatment of OA.

结论骨性关节炎软骨细胞在骨性关节炎发病过程中,软骨细胞正常分化及增殖发生改变;骨性关节炎软骨基质降解过程可能是金属蛋白酶与其它因子协同作用的结果;鹿茸多肽可促进骨性关节炎软骨细胞增殖,并可抑制骨性关节炎软骨细胞中金属蛋白酶的过度表达,可能对治疗骨性关节炎有较好的作用。

In electricity plus thermal group, the pathologic alterations were slighter than model group; there was little crack on the surface of cartilage, with intact collagen fiber structure and less pyknotic chondrocytes. Although some chondrocytes shrank, there were intact cell organs in many chondrocytes even chondrocyte cluster in some regions.

疏密波+热软膜组在病理程度上的改变明显轻于模型对照组,软骨表面裂纹少,胶原纤维结构基本完整,固缩的软骨细胞少,虽可见部分退变的软骨细胞,但部分软骨细胞具有较多的细胞器,某些区域形成软骨细胞簇。

PART ONE CONSTRUCTION OF VECTOR CARRYING HUMAN INSULIN-LIKE GROWTH FACTOR-1 GENE AND THE GENE EXPRESSION IN EUCARYOTE Insulin-like growth factor-1(IGF-1) is one of the most important regulate factors which can stimulate cartilage cell to differentiate and synthesis collagen typeⅡand proteoglycan to maintain cartilage phaenotype.

第一部分:携带hIGF-1基因重组载体的构建及在真核细胞的表达胰岛素样生长因子-1(IGF-1)是在软骨发育和软骨自稳态调节中最重要的生长因子之一,能刺激软骨细胞分裂增殖,并促进软骨细胞合成Ⅱ型胶原和蛋白聚糖,维持软骨细胞的表型等。

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