软骨样的

Methods Cartilaginous tissues of knee joint were obtained from 6 patients with OA and 5 patients with traumatic amputation.

目的 研究骨性关节炎软骨组织中细胞因子胰岛素样生长因子Ⅱ的基因表达的变化规律。

In order to test this hypothesis, we isolated and developed stable self-renewable cell lines of mesenchymal stem cells from human fetal articular cartilage and identified the cell phenotype by FACS and we found that their phenotypic markers were identical to those of mesenchymal stem cells from bone marrow.

我们从胚胎的关节软骨中获得成纤维样细胞，在体外进行培养和扩增，并用流式分析的方法对其表面标志进行鉴定，发现其表面标志与文献报道的骨髓来源的间充质干细胞的表面标志相同。

Objective To study the genetic expression of insulinlike growth factor Ⅱ in cartilaginous tissue in osteoarthritis.

目的 研究骨性关节炎软骨组织中细胞因子胰岛素样生长因子Ⅱ的基因表达的变化规律。

Of note, this study is the first to characterize the D2-40 immunoprofile in extraskeletal myxoid chondrosarcoma, results that could be of utility in differential diagnostic assessment.

值得注意的是，本研究是文献中第一次描述D2-40如何在骨外黏液样软骨肉瘤中表达，这个结果可以应用于我们今后的鉴别诊断当中。

Cumulative data have been documented that mesenchymal stem cells can differentiate into osteoblasts, chondrocytes, adipocytes, tenocytes, myotubes, neural-like cells, hematopoietic-supporting stroma and other non-hematopoietic cells derived from mesoblast or ectoblast.

大量研究表明，体外培养的骨髓间质干细胞在合适的诱导条件下，可分化为成骨细胞、成软骨细胞、脂肪细胞、成肌细胞、成腱细胞和神经元样细胞等多种非造血组织特别是中胚层和神经外胚层来源的组织细胞。

More clones expressed phenotypes of osteoblasts (48%), chondrocytes (43%), and neuron-like cells (52%) than of adipocytes (12%), possibly due to the loss of adipogenic ability after repeated subcultures.

多数克隆的表型是成骨细胞（48%）、软骨细胞（43%）、神经元样细胞（52%），而脂肪细胞表型的克隆仅12%，这可能由于多次传代培养后成脂能力的丧失。

Combination TGF-β3 with IGF-I can induce the MSCs capsulated in alginate microspheres to differentiate into chondrocytes, and chitosan-based scaffold is biocompatible with the induced chondrocytes.

转化生长因子β3和胰岛素样生长因子Ⅰ能诱导藻酸钠微球中的骨髓间充质干细胞定向分化成软骨细胞，并与壳聚糖复合支架表现出良好的组织相容性。

Results: The cultured chondrocytes were polygonal cells. There were many rough endoplasmic reticula and mitochondria in cytoplasm, and a lot of secretory vesicles under cell membrane and in the cytoplasm. When cultured for 10 days, some small and white nodules were formed on the bottom of the culture dished, and volcanic-mouth-like structures were formed when cultured for 20 days. Both these nodules and structures contained GAG-positive substances were demonstrated by safranine-O staining.

结果：体外培养的软骨细胞为多角形，透射电镜下见胞浆内有丰富的粗面内质网系统及线粒体，胞膜下及胞浆中有较多分泌泡；连续培养10天时，培养瓶底部出现肉眼可见的白色结节，蕃红-O染色显示白色结节含大量氨基葡聚糖阳性物质，20天时形成火山口样结构，也被蕃红-O染成深红色。

The histopathologic changes included lymphocyte and monocyte infiltration,hyperplasia of synovial cells and small vessels,interstitial fibrosis,hyaline degeneration and cartilaginous metaplasia.The immunohistochemical observations showed that the high expressions of IL-1 and IL-6 were significantly different between the pathologic plicae and the control groups（P＜0.01）.The positive expressions of IL-1 and IL-6 were the synovial cells and monocyt-lymph cells in the pathologic synovial plicae.The positive expressions of MMP-1 and TIMP-1 have significant difference between the experiment and control group（P＜0.01,P＜0.05）.The expression of MMP-1 was positive in synovial lining cell,monocyte,fibroblast,endothelial cell in small vessel and chondrocyte.The TIMP-1 expression was detected in the synovial lining cells and a small quantity fibroblast.

结果 正常滑膜皱襞和病理性滑膜皱襞在滑膜细胞增生及小血管增生、间质纤维化及玻璃样变、软骨化生组织学改变方面，差异均有显著性（P＜0.01）；IL-1、IL-6在病理性滑膜皱襞内的增生滑膜细胞、单核及淋巴细胞和在正常滑膜皱襞内的表达差异均有显著性（P＜0.01）； MMP-1、TIMP-1在病理性滑膜皱襞和正常皱襞内的阳性表达，差异具有显著性（P＜0.01，P＜0.05），MMP-1在增生滑膜衬里层细胞、单核和纤维母细胞、血管内皮细胞和软骨化生的软骨细胞呈阳性表达；TIMP-1只在滑膜衬里层细胞和少量纤维母细胞有表达。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷