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Results The tissue engineered tissue from 1 week group is more like fibrocartilage. From 2 week group,the tissue gradually transfer into hyaline cartilage and form more mature hyaline like cartilage in 4 weeks group.

结果:经组织学染色发现:在诱导的初期组织更偏向于纤维软骨,而从第二周开始组织逐渐向透明软骨转化,至第4周逐渐形成成熟的透明软骨样组织。

A new parallel-epiphyseal cartilaginous tissue was found in the subcutaneous tissue 4 weeks after operation. The tissue was stained with HE, toluidine blue, safranine O and collagenⅡ. A large number of chondrocytes and a cartilage lacuna-like structure were observed under a microscope with no obvious inflammatory reaction around the calcium alginate and polylysine microspheres.

结果 体外培养时微球中骺板细胞生长、增殖情况良好;移植皮下后第4周取材可见外形呈类骺软骨样组织块,行HE、甲苯胺蓝、番红"O"及Ⅱ型胶原等染色,镜下观察可见大量软骨细胞及类软骨陷窝样结构,植入的海藻酸钙微球周围组织无明显炎症反应。

After 12 weeks,the result showed the operation area of group A articular surface was smooth,with the surrounding normal cartilage naturally straight flush, transparent form new cartilage tissue;Group B restoration surgery the basic integrity of the cartilage tissue,but the center of many not fully integrated,there is slight depression,surface wear,good subchondral bone formation;control group, depression joint operation areas,non-articular cartilage formation,such as lack of bone joints.

术后12周取材,A组修复区可见部分软骨样组织,关节软骨无磨损,修复的软骨呈白色半透明外观,与周围正常关节软骨有连续性,可见一明显的凹陷,无明显软骨下骨外露;B组修复区也可见部分软骨样组织,关节软骨在修复区可见磨损,软骨呈白色不透明外观,软骨下骨形成较好;空白对照组术区关节凹陷,无关节软骨组织形成,关节下骨缺如。

The defects of articular cartilage were repaired with cartilage without chondral degenerations in group A, which was similar to the normal chondral structure.

结果:B、C组软骨损伤区形成骨纤维组织修复,术后12周,C组出现明显的软骨退行性变,B组无明显退变:A组软骨缺损区形成软骨样组织修复,且与邻近正常软骨层相连续,未见明显的软骨退变。

RESULTS: In the SH-rhBMP-2 group, sparse chondroid tissue could be found at the 2 weeks of operation, which arranged orderly along the tibial tunnel from 4 to 8 weeks, tendon adhered to adjacent tissue stably, and bony tissue was appeared at the 12 weeks.

结果:透明质酸钠-重组人骨形态发生蛋白2亚组在术后2周隧道内见稀疏软骨样组织,4~8周时隧道内见沿骨隧道排列的软骨样组织,双股肌腱之间形成稳定组织连接。l2周时出现部分骨样组织包裹肌腱。

The cartilage of germinative layer and basal layer grew better than that in bfgf group, magnetic group.

结果表明:磁场结合bfgf组在4周时基底层的软骨样组织增生,而磁场组、bfgf组少量软骨样组织增生。8、12周结合组透明样软骨层明显增厚,免疫组化ⅱ型胶原多。

For both genes, highest expression was found for costal chondrocytes, followed by nasoseptal, articular, and auricular cells. Retrieal of 20-week discs from mice reealed changes in construct dimensions with different chondrocytes.

就同一种基因而言,肋软骨来源的软骨细胞表达最高,其次是鼻中隔,关节以及耳。20周后从裸鼠皮下回收的不同软骨细胞来源的圆盘样组织在体积上也有变化。

PART ONE CONSTRUCTION OF VECTOR CARRYING HUMAN INSULIN-LIKE GROWTH FACTOR-1 GENE AND THE GENE EXPRESSION IN EUCARYOTE Insulin-like growth factor-1(IGF-1) is one of the most important regulate factors which can stimulate cartilage cell to differentiate and synthesis collagen typeⅡand proteoglycan to maintain cartilage phaenotype.

第一部分:携带hIGF-1基因重组载体的构建及在真核细胞的表达胰岛素样生长因子-1(IGF-1)是在软骨发育和软骨自稳态调节中最重要的生长因子之一,能刺激软骨细胞分裂增殖,并促进软骨细胞合成Ⅱ型胶原和蛋白聚糖,维持软骨细胞的表型等。

The cartilaginous cap contained hyaline cartilage that retained its lacunar pattern.

软骨帽中有平顶样的透明软骨。

The core technology of tissue engineering is utilize the suitable seed-cells compound with organic materials to build a cartilage-like tissue in vitro or in vivo, this tissue must be similar with hyaline cartilage in forms、structure and behavior.

软骨组织工程的核心技术就是利用合适的种子细胞复合生物材料,在体内或体外构建与人关节透明软骨形态、结构、性能相似的软骨样组织,用于软骨移植。

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