软骨形成

Its ability of chondrogenesis is increased evidently in cultured by diplex growth factors.

结论MSCs经分离扩增后种植于三维立体结构的纤维蛋白海绵载体上，在含有TGF β1培养液中形成软骨；在TGF β1和FGF的双重因子培养中，其软骨的形成能力明显增加。

Gross observation and mass changes of the grafts: Two weeks after inoculation, there were obvious nod formations at the inoculated sites, but no nod formed in the blank control group.

移植物的大体观察与质量变化：接种2周后，同种异体软骨细胞-FasL组及同种异体软骨细胞组兔接种细胞-材料复合物处，均有明显的结节形成，对照组未形成接节。

Through dynamic observation in the morphology with a microscope, we first proved that osteophyte arises from the proliferation of peripheral articular cartilage and further endochondral ossification.

我们在显微镜下通过对骨赘形成的动态观察，首次证明骨赘是由周边关节软骨细胞增殖，再经软骨内化骨而形成的。

At the 110th day,the semitransparent cartilage discs were 4.4 mm in diameter and 14.8mg in weightness.

结果 培养21天时形成"膜状软骨"，至110天时形成直径约为4.4 mm的"圆盘状软骨"。

At 12 week, the cartilage surface of the MSCs/ HA/CPP/PLLA group was smooth, cartilage layer with normal thickness and complete subcartilaginous bone were observed with light microscope, which had significant differences compared with the control group.

细胞材料复合物组12周关节软骨表面光滑，光镜下可见已形成正常软骨厚度的软骨层及完整的软骨下骨，与对照组有明显差异。

Then, under the control of systemic factors, mesenchymal cells aggregate and differentiate to form catilage blastemal elements that prefigure skeletal limb components.

脊椎动物胚胎期骨与关节系统的发生是一种复杂生命现象，起始于中胚层间充质细胞的定向聚集，形成肢芽，然后在一系列作用因子的调控下，肢芽内细胞进一步分化，形成具有骨骼雏形的软骨原基，后者经软骨内骨化发育成骨。

Therefore to study the molecule regulation mechanism of development and maturation of condylar cartilage in embryo, can not only discover the mechanism of its physiological rebuilding and restoration to set up the academic base for related treatment in clinic, but possiblely supply new treatment methods for trauma and destruction to articular cartilage. Histological and immunohistochemistry methods have been used to state the feature of development of the mandibular condylar cartilage, and molecule mechanism of its endochondral ossification.

目前对四肢关节软骨、生长板软骨的发生及再生修复的研究较为深入，而髁突软骨的发生、生理改建及再生修复机制的研究尚处于起步阶段，因此研究胚胎期髁突软骨发育及形成的分子调控机制，可望揭示其生理性改建及损伤修复的机制，为临床有关治疗奠定理论基础，并可能为关节软骨的损伤及破坏提供新的治疗手段。

In electricity plus thermal group, the pathologic alterations were slighter than model group; there was little crack on the surface of cartilage, with intact collagen fiber structure and less pyknotic chondrocytes. Although some chondrocytes shrank, there were intact cell organs in many chondrocytes even chondrocyte cluster in some regions.

疏密波+热软膜组在病理程度上的改变明显轻于模型对照组，软骨表面裂纹少，胶原纤维结构基本完整，固缩的软骨细胞少，虽可见部分退变的软骨细胞，但部分软骨细胞具有较多的细胞器，某些区域形成软骨细胞簇。

RESULTS: During 10 - 12 weeks, in cuntrol group: The defect area was repaired by white and soft tissue that had no resistance to press. The repaired tissue was still lower than the surrounding articular surface with clear boundary. By histological observation, it was found that the defect was repaired by the mechanism similar to inflammatory reaction and the defect is ultimately filled by the hyperplasia of hyaline degenerative fibrous tissues. In filling group: the defect was repaired by semi-transparent, smooth, textured tissues with polish that had resistance to press as well as elasticity. The repaired tissue was almost similar to the shape of the surrounding cartilage,difficult to be distinguished. After histological observation, it was found that there was no inflammatory reaction, but active hyperplasia of inner bonetissue and cartilage tissues; a lot of osteoid tissues and trabeculation were found. Newlborn cartilage was fused with surrounding cartilage tissue and connected with surrounding tissues.

结果：10～12周，对照组：缺损区由白色、质软、按压无阻抗的组织修复，修复组织仍低于周围关节面，边界仍清晰可辨，组织学以类似炎症反应的机制修复缺损，最终以透明变性的纤维组织的增生来填补缺损部位；填充组：缺损区由半透明状、质韧光滑有光泽，按压有阻抗并有弹性的组织修复，修复组织与周围软骨外形上已基本相似，不易区分，组织学未见有炎症反应的过程，内骨组织和软骨组织增生活跃，并可见大量类骨组织和骨小梁形成，新生软骨和周围软骨组织融合，并与周围组织连接。

During operation, an intranasal and marginal combining incision was made to expl ore the alar cartilages and to from a mucosal-cartilage flap in the nasal vest ibule. After the deformity of septal cartilage and the abnormalities of the alar base on the cleft side were repaired, the total alar cartilage was repositioned and rotated with suspension and V-Y advancement to correct the nasal deformiti es. Results Since 1993, a total of 92 cases were treated by the above p rocedure and satisfactory results were obtained.

手术经鼻端和患侧前庭联合切口，解剖、显露双侧鼻翼软骨，同时形成患侧前庭粘膜软骨瓣；在纠正鼻中隔软骨偏曲，松解患侧鼻肌复合体起点，使鼻翼脚无张力抬高后，再通过悬吊技术和粘膜软骨瓣的V-Y推进，将患侧鼻翼软骨进行整体旋转复位；最后，应用口轮匝肌上部纤维内收抬高鼻翼脚，用鼻肌复合体起点复位调整鼻孔形态，完成鼻畸形的矫正。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。