软骨内的

Objective:To investigate in vitro chondrogenic ability of chondrocytes in fibrin gel.

目的 ：研究软骨细胞在纤维胶内体外生成组织工程化软骨的可行性。

Results: Arthroscopic examination showed that there were synovitis, debris and fibrillation in the joints of ADD with reduction; synovitis, synovial hyperplasia, debris, fibrillation, chondromalacia,fibrocartilage rupture and adhision in the joints of ADD without reduction.

结果：可复性关节盘前移位的病例出现滑膜炎，关节腔内有絮状物，关节结节表面有纤维形成。不可复性关节结节表面纤维形成，关节软骨软化，软骨剥脱，同时伴有纤维粘连。

These include inflammatory arthritides such as rheumatoid arthritis, ankylosing spondylitis and reactive arthritis,99 101 synovial impingement,meniscoid entrapment, chondromalacia facetae, pseudogout,synovial inflammation, villonodular synovitis, and acute and chronic infection.102 106 Intrafacetal synovial cysts can be a source of pain because of distension and pressure on adjacent pain-generating structures, calcification,and asymmetrical facet hypertrophy.107 110 In a retrospective review of MRI scans in 303 consecutive patients with LBP, Doyle and Merrilees111 found that9.5% had facet joint synovial cysts, the large majority of which were located posteriorly.

包括炎症性关节炎如类风湿性关节炎，强直性脊柱炎及反应性关节炎等，还有滑液撞击，关节软骨版卡压，关节面软骨软化，假性痛风，滑液炎症，绒毛结节性滑膜炎和急慢性感染。小关节内滑囊囊肿可由于拉伸和压缩临近的疼痛发生结构，骨化及不对称关节增生等原因引起疼痛。在一项303例连续患者的MRI研究中，Doyle 和 Merrilees发现其中9.5%存在小关节囊肿，大部分发生于关节囊后部。

The study of bipolar radiofrequency energy chondropalsty to degenerative articular cartilage of goat We performed bipolar frequency energy chondroplasty to degenerative articular cartilage three months after the first operation.

使用双极射频（Arthrocarc system 2000，射频头是CoVac 50），能级设定为1，对右膝退变的髌骨和股骨内髁软骨进行软骨成形术，左膝进行假手术作为对照。

P=0.002 .(2) Histological results demonstrated: the large area of fibrosis of cartilage could be seen in the medial condyle at 26 weeks; the fibrosis was deep into the deep layer of cartilage and the number of cells decreased; the average Mankin's score was 7. 7, which significantly increased with that in controlled group.

2后交叉韧带切断后26周内髁软骨可见范围较大的软骨纤维化现象，深达深层，细胞数量减少，Mankin评分平均7.7，和对照组同期相比有明显的统计学意义（Hc=8.486，P=0.004）。

All rats were sacrificed at 12 weeks after operation. the macro-pathological changes of samples were graded by mankin's scale.bone mineral density of right distal femoras and femora condyles were measured by dual-energy x-ray absorptiometry scanner.

术后12周处死动物取材，采用mankin评分系统评分；用双能骨密度仪测量右侧股骨远端1/4骨密度和股骨内外侧髁软骨下骨的骨密度；右侧股骨髁脱钙后切片，行番红&o&染色和mmp-13免疫组化染色；右侧胫骨近端做硬组织切片，测量软骨下松质骨的骨组织形态计量学参数。

TyPe II collagen induced arthritisln the rat ank1e joint andoVathumin as antigen induced arthritis WA in the rabbit knee joint wereestab1ish2 Qualitative evaluation of me in skin, muscle, synovium, cedilagearound joint and blood was performed by OMA3 The CIA rats were treated on day 7 after hind paw swelling and erythemaAnimals were injected intravenously with ase at a dose of 10mg/kg,tWenty minuots 1ater, one ankle of the rats random1y assigned was exPosedlaser irradiation at l00J/cm fOr l000 seconds, and another ankle wasM grouP wihout laser The other two groups is unmanipulatedcontrol group and untreated CIA group Bimaleolar ankle widthmeasuremellts were taken in all animals every tWo days using amicrometer The histopathology of the ank1e Joint was assessed at day 21after disease onset4 The pro1iferating cell nuclear antigen WCNA of CIA treated by PDT andthe HMME group without laser was doterdrined by immunohistochemiStry5 The AfA rabbits were treated on day 7 after knee swelling and erythemaThe theraPy invo1ved lntravenous injection of l0mg/kg HMME, fOl1owedby 20 minues period in dim light, and transdermal light treatment with\l00 J/cm2 fOr l000 seconds The inner sides of the treated Anees wereirradiated at first, and then the outer side did 24 hours later, the synovialtissue of the Anees joint were removed and in situ cel1 aPoptosis wasdetCCted With tednal deoxync1eotidyl transferase-mediated dUTP nickend labelingR6suIt8:l The pathologic changes of CIA and AIA include subsynovial inflammation,opovial hyPerplasia, pannus formation, cartilage and bone destructionresemble RA.2 The studies demonstrated that there are different uptake of HMME withinskin, muscle, synovium, cartilage and b1ood, and the synovium cou1draPidly uPtake more ase than skin and cartilage at the firSt 30 minuesaller intravenous injection of HMME3 The bimaleolar anke width had no different among PDT treated group,H group withollt 1aser and untreated CIA group But hlstologicalevaluation showed statiStical1y significallt reductions in synovialhyperplasia, pannus formation and cart1lage reosion, bone destruction andtotal score in PDT treated group4 Image analysis showed that the ratlo bforeen the areas of the coufltedobect to that of the entire area in PDTtreated grOup is lower than that in conirol group, but the integrated oPticaldensity had no different between the two groups5 Imape analysis showed that the ratio between the area of the countedobject to that of the e

治疗组在大鼠出现踝关节红肿后1周，炎症达到高峰时进行PDT治疗。随机治疗大鼠一侧的踝关节，另。2。一一侧作单纯HMME 对照。治疗方法是大鼠麻醉后尾静脉注入 HMME10ngkg，20分钟后踝关节照光，激光波长627.sum，功率密度 100mwcm'，照射时间1000秒，能量密度100）／。治疗后避光喂养72 小时。隔日一次测量大鼠的踝关节左右横径，治疗后两周取关节进行病理d 观察。 4。大鼠CIA模型用上述方法进行PDT治疗后，治疗组和单纯HMME 组用兔疫组化SP法检测石蜡切片的核增殖抗原。 5。兔AIA模型在关节炎出现第七天进行PDT治疗，随机治疗一侧膝关节，另一侧作自身对照。兔耳静脉注入I'arrainrelomg／Kg，20分钟后，膝关节用金蒸气激光照射，激光能量密度100）儿旷。24 ／J'时后取膝关节滑膜作病理检查，并用脱氧核昔酸末端转移酶介导的缺口末端标记法原位检测凋亡细胞。结果： 1。模型观察：CIA大鼠炎症高峰期滑膜下炎细胞浸润明显，滑膜细胞明显增殖，炎症达到高峰后二周，血管缀形成，并侵蚀和破坏软骨和骨， CIA模型病理改变与人类RA相似。兔AIA模型膝关节滑膜病理可见滑膜细胞增生，滑膜下炎细胞浸润，也与人类RA滑膜改变相似。 2。关节周围组织中光敏剂含量的测定结果表明，各组织对HMME 的吸收速度和吸收量不同，荧光值一时间曲线不同，滑膜组织比皮肤和软骨对 HMME的吸收多，在 2 0分钟时即有明显差异。 3.PDT对CIA模型的治疗结果表明：PDT治疗后关节炎组、单纯 HMME组和治疗组踝关节左右横径统计学检验差异没有显著性，但病理评分PDT治疗组滑膜增生、血管资形成及软骨破坏、骨破坏和总分比关节炎对照组和HMME对照组好，统计学检验差异有显著性。。3＿军医进修学院硕士学位论文中文摘要 4.PDT治疗组PCNA阳性细胞较对照组少，图像分析结果表明面密度（阳性染色的面积总和与统计视野面积的比值）治疗组小于对照组，统计学检验差异有显著性。。 5.PDT治疗组凋亡阳性细胞较对照组明显增多，图像分析结果单位视野内阳性细胞数和面密度PDT治疗组高于对照组，统计学检验差异有显著性。凋亡细胞核直径PDT治疗组较小，与对照组相比，统计学检验差异有显著性。结论：二。CIA、AIA的病理改变类似人类RA，可作为研究RA病因、发病机制、检查及治疗方法的模型。 2。各组织对HMME的吸收速度和吸收量不同，滑膜组织比皮。

Evaluate MRI representation:(1) segregation condition: transfixation band, segregation between cartilage and bone, segregation between bone and bone;(2) four stages of pathologic classification based on MRI signal character;(3) texture in weight bearing zone;(4) add Bone Marrow Edema score and joint effusion score as stasis index.

术前MRI评估：(1)股骨头内分离情况：贯穿带、软骨与骨分离，软骨下骨与骨分离；(2)股骨头内信号的病理分期；(3)冠状正中位的髋臼负重区下结构；(4)将股骨头骨髓水肿评分和关节积液评分相加记为关节的瘀积指数。

Methods 65 cases（74 knee） of senile osteoarthritis of the knee were debrided under arthroscopy with local anesthesia.The procedures included removal of hyperplastic membrance and raw surface of degenerated cartilage,repairing the meniscus,and reshaping of the intercondylar fossa.

对65例（74膝）膝关节骨性关节炎患者采用局麻下行关节清理术，手术清理增生炎性滑膜，清理退变剥脱的软骨及打磨软骨创面，修整损伤半月板，取出关节内游离体、碎骨屑及炎性介质，髁间窝成形术解除撞击及磨损前交叉韧带。

Abstractive】 objective to investigate the diagnostic superiorty and therapeutic efficiancy of arthroscopic debridement for the osteoarthritis of the knee.methods 65 cases（74 knee） of senile osteoarthritis of the knee were debrided under arthroscopy with local anesthesia.the procedures included removal of hyperplastic membrance and raw surface of degenerated cartilage,repairing the meniscus,and reshaping of the intercondylar fossa.results 55 cases（67 knee） were followed up for 20 months in average.the pain was relieved and the function of the knee joint was restored in most patients.

目的 探讨关节镜下清理术治疗骨性关节炎的诊断价值及治疗效果。方法对65例（74膝）膝关节骨性关节炎患者采用局麻下行关节清理术，手术清理增生炎性滑膜，清理退变剥脱的软骨及打磨软骨创面，修整损伤半月板，取出关节内游离体、碎骨屑及炎性介质，髁间窝成形术解除撞击及磨损前交叉韧带。结果随诊55例（67膝），随访时间6～42个月，平均20个月，多数患者临床症状消失或者疼痛症状减轻，关节功能明显改善。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新