软骨内的

In the normal group, electron microscopy showed normal chondrocytes were oval, and cells and cell membrane were intact. In cytoplasm, there were abundant rough endoplasmic reticulum, dictyosome, and mitochondria with intact nucleus, and even chromatin. In the model group, chondrocytes exhibited obvious pyknosis and their shape was irregular. The areolae around cells disappeared; cell organs in cytoplasm coagulated to flaps which showed electron dense and was uneasy to observe; the shape of nucleus showed irregularity, and caryotin gathered densely.

电镜下正常对照组软骨细胞呈椭圆形，细胞及胞膜完整，包质内可见丰富的粗面内质网、高尔基体、线粒体，细胞核完整，染色质分布均匀；模型对照组软骨细胞明显固缩且外形不规则，细胞周晕消失，胞浆内细胞器凝成高电子密度的片状物不易分辨，细胞核不规则，染色质浓聚，散裂于核中。

Our findings demonstrate that in conjunction with clinical and radiographic findings, immunohistochemical evaluation with a panel of D2-40, EMA, brachyury, and GFAP is most useful in distinguishing chordoid meningioma from chordoid glioma, skeletal myxoid chondrosarcoma, extraskeletal myxoid chondrosarcoma, chordoma, low-grade chondrosarcoma, and enchondroma. A lack of strong, diffuse S100 reactivity may also be useful in excluding chordoid meningioma.

我们的结果表明，结合临床和影像学资料，D2-40、EMA、brachyury和GFAP这样一个免疫标记组合就能很好地鉴别脊索样脑膜瘤和脊索样胶质瘤、骨的黏液样软骨肉瘤、骨外的黏液样软骨肉瘤、脊索瘤、低级别软骨肉瘤、内生性软骨瘤。S100染色不强烈、不弥漫也有助于排除脊索样脑膜瘤的诊断。

EMA was the most effective antibody for differentiating chordoid meningioma from skeletal myxoid chondrosarcoma, low-grade chondrosarcoma, and enchondroma, whereas D2-40 was the most effective antibody for differentiating chordoid meningioma from extraskeletal myxoid chondrosarcoma and chordoma.

EMA是鉴别脊索样脑膜瘤和骨的黏液样软骨肉瘤、低级别软骨肉瘤、内生性软骨瘤最有效的抗体，而D2-40则是鉴别脊索样脑膜瘤和骨外黏液样软骨肉瘤、脊索瘤最有效的抗体。

Material and MethodsRA Rabbit Model group: 15 early RA rabbits of the same weight and variety Control group:10 normal rabbits of the same weight and varietyMethods of making RA rabbit model:elect 15 normal rabbits of the same weight and variety, dissolve Ovalbumin in 0.9% sodium chloride solution, to make the solution of 20mg/ml concengtration, blend the same quantity of complete Freund′s Adjuvant equably ,inject the mixed solution into endermic tissue of the rabbit′s scapular section, making the rabbits allergic, inject 1 ml of the mixed solution to one rabbit every time, inject 1 ml of the mixed solution in 5 different places of the rabbit′s scapular section, inject the rabbit one time every week,inject 3 weeks continuously,it turned out to be that the rabbits will be allergic, inject Ovalbumin blent with the 0.9% sodium chloride solution into the knee joint cavity of the rabbits in the fourth week, 5 mg Ovalbumin every knee joint cavity,two knees of every rabbit of the 15 rabbits are both injected,the arthrosis diameter and the exterior temperature of the knees will be added obviously in 24 hours,and they will drop gradually,at the time of the 14th or 21th day, the arthrosis diameter and the exterior temperature of the knees will get to the balance time,the incidence rate of RA is 100%.after the RA model succeeds,it is the early time of RA from the first week to the fourth week,after the fourth week,it is the late time of RA, the cartilage of the femoral condyle and the subchondral bone cortices will be changed unrecoverily.

资料与方法RA模型组：早期RA家兔模型15只，品种及体重接近。对照组：正常家兔10只，品种及体重接近。RA家兔模型制作方法：选取15只成年家兔，体重、品种接近，将卵蛋白溶解于生理盐水，配成浓度为20mg/ml的溶液，与等量完全弗氏佐剂混匀，注入家兔肩胛区皮下致敏，每只家兔每次注射1ml，于肩胛区5个不同区域注射，每周一次，连续注射3周而致敏，第4周向膝关节腔注射卵蛋白生理盐水溶液，每只关节腔注射5mg卵蛋白，15只家兔膝关节全部注射，24h内此关节直径和表面温度大幅度上升，以后缓慢下降，至14～21d达到平台期，发病率达100%。造模成功后第1～4周为早期改变，第4周以后出现不可逆的关节软骨及骨破坏。采用高频超声对RA模型组与对照组的膝关节髌上囊液体厚度、滑膜及股骨髁软骨厚度及软骨下骨皮质的回声情况进行对比观察。结果RA组模型组膝关节髌上囊积液及滑膜的厚度明显厚于对照组，其股骨髁软骨的厚度与对照组相比无明显差异，其软骨下骨皮质与对照组相比无明显改变。

At early stage after brain injury, there were a quantity of fibrous callus and cartilaginous callus formation in brain injury and fracture group and many neuropeptides immunoreactive nerve fibers in callus were found. Strong immunoreactivites of CGRP, SP, VIP, NPY, TOH occurred to osteogenitor cells and chondroblast, which proliferated in thickened endothecium.

脑损伤合并骨折组早期形成大量纤维骨痂和软骨骨痂，骨痂中神经肽免疫阳性神经纤维较多，明显增厚的骨膜内层骨祖细胞、幼稚的软骨细胞胞质内降钙素基因相关肽、P物质、血管活性肠肽、酪氨酸羟化酶、神经肽Y强阳性表达。

Articular chondrocytes from the knee joints of the A group were separated respectively to fragments, and then digested by enzymes, both the chondrocytes and the uncomplite digested tiny chondral tissues have been cultured in vitro for about 2 weeks.

分别取A组9只兔子的双侧膝关节股骨滑车及部分髁部的软骨，在体外进行分离和消化，未完全消化的软骨组织加软骨细胞在培养瓶内一同培养2周后配制成浓度为1.6×10~8/ml的软骨细胞悬液，等分装于两个EP管中。

RESULTS: In the SH-rhBMP-2 group, sparse chondroid tissue could be found at the 2 weeks of operation, which arranged orderly along the tibial tunnel from 4 to 8 weeks, tendon adhered to adjacent tissue stably, and bony tissue was appeared at the 12 weeks.

结果：透明质酸钠-重组人骨形态发生蛋白2亚组在术后2周隧道内见稀疏软骨样组织，4~8周时隧道内见沿骨隧道排列的软骨样组织，双股肌腱之间形成稳定组织连接。l2周时出现部分骨样组织包裹肌腱。

In this study, immunohistochemical staining was performed with antibodies against D2-40, S100, pankeratin, epithelial membrane antigen, brachyury, and glial fibrillary acidic protein in 4 cases of chordoid glioma, 6 skeletal myxoid chondrosarcomas, 10 chordoid meningiomas, 16 extraskeletal myxoid chondrosarcoma, 18 chordomas, 22 low-grade chondrosarcomas, and 27 enchondromas.

本研究中，我们给4例脊索样胶质瘤、6例骨的黏液样软骨肉瘤、10脊索样脑膜瘤、16例骨外黏液样软骨肉瘤、18例脊索瘤、22例低级别软骨肉瘤和27例内生性软骨瘤做了D2-40、S100、pankeratin、上皮膜抗原、brachyury和胶质纤维酸性蛋白的免疫组化染色。

Immunohistochemistrical section: observe the VEGF protein expression in different cells, the positive ratio detected by a morphometrical analysis system was used as the amount of the VEGF protein expression.

免疫组化染色切片：观察不同给药时间股骨头内VEGF的表达情况；高倍视野下（400倍）沿骨小梁表面计数50个成骨细胞中的阳性细胞数，VEGF成骨细胞阳性率=阳性成骨细胞数/50×100％，3次取平均值；计数每个高倍视野软骨细胞中的阳性细胞数，VEGF软骨细胞阳性率=阳性软骨细胞数/软骨细胞总数×100％，5次取平均值。

RESULTS: During 10 - 12 weeks, in cuntrol group: The defect area was repaired by white and soft tissue that had no resistance to press. The repaired tissue was still lower than the surrounding articular surface with clear boundary. By histological observation, it was found that the defect was repaired by the mechanism similar to inflammatory reaction and the defect is ultimately filled by the hyperplasia of hyaline degenerative fibrous tissues. In filling group: the defect was repaired by semi-transparent, smooth, textured tissues with polish that had resistance to press as well as elasticity. The repaired tissue was almost similar to the shape of the surrounding cartilage,difficult to be distinguished. After histological observation, it was found that there was no inflammatory reaction, but active hyperplasia of inner bonetissue and cartilage tissues; a lot of osteoid tissues and trabeculation were found. Newlborn cartilage was fused with surrounding cartilage tissue and connected with surrounding tissues.

结果：10～12周，对照组：缺损区由白色、质软、按压无阻抗的组织修复，修复组织仍低于周围关节面，边界仍清晰可辨，组织学以类似炎症反应的机制修复缺损，最终以透明变性的纤维组织的增生来填补缺损部位；填充组：缺损区由半透明状、质韧光滑有光泽，按压有阻抗并有弹性的组织修复，修复组织与周围软骨外形上已基本相似，不易区分，组织学未见有炎症反应的过程，内骨组织和软骨组织增生活跃，并可见大量类骨组织和骨小梁形成，新生软骨和周围软骨组织融合，并与周围组织连接。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新