软体

About the Company for the sale of high-quality professional Velcro, the main products: Standard (up to a maximum width of 150 MM-160MM), not carding type, and sulfur-resistant, soft hook-type, soft hook Ruanjiao Type, high frequency type, Beijiao; Beijiao-high temperature, water-resistant, fire zone, with the hook of hair, hair with hook-and elastic Velcro series; special color series; cloth Moshu Tie Series; PVC plastic hook; special processing zone series.

公司简介本公司为专业销售高品质粘扣带，主要产品有：标准型（最宽可达150MM-160MM）、不梳毛型、耐加硫型、软勾型、软勾软胶型、高周波型、背胶型；高温背胶型、防水型、防火带型、勾毛同体、勾毛同面、松紧粘扣带系列；特殊色系列；魔术贴布系列；PVC塑料勾；特殊加工带系列。

The lower lithospheric mantle in western Shandong could be composed of Group Ⅰ inclusions which were subjected to orthopyroxene vein or orthopyroxene+ phlogopite vein and disseminated phlogopite metasomatism.

鲁西区软流圈的顶部，即岩石圈地幔的底部应为一套组Ⅰ型包体，它们存在斜方辉石交代脉体和斜方辉石+金云母的交代脉体以及弥漫型金云母；向上(约43～57km±)为一套堆积岩。

In juveniles, the structure of the tissues in the mesoyl layer were looser than the surface layer and there were more black chromatocytes in the surface layer than the mesohyl layer. The compositions of spicules were different in various stages of the sponge. There were no spicules in embryos and larvae. In juveniles, microscleres were the main components. However, megascleres of oxea and strongyle were the major types in adult.

在骨针组成方面，黑色软海绵随不同发育时期而有差异，在胚胎以及幼生阶段体内皆无骨针存在，白色及黑色幼体体内之骨针的组成为小型骨针，有oxyaster euaster及 oxyaster oxyspheraster两型，以及大型骨针尖杆体及二辐钝端骨针两型；而黑色软海绵成体的骨针组成为大型骨针，并未发现存在於幼体体内之小型骨针。

The results show that:(1) after MA for 20h, single phase bcc nanocrystalline supersaturate solid solution formed in Fe84Nb7B9 and Fe80Ti8B12 alloys; the thermal stability of nanocrystalline microstructure in Fe80Ti8B12 alloy was improved significantly than that in Fe84Nb7B9 alloy, and the decomposition temperature of supersaturate solid solution decreased in Fe80Ti8B12 alloy.(2) under 30MPa/900℃/0.5h hot-press sintering conditions, the relative density of Fe84Nb7B9 and Fe80Ti8B12 bulk alloys were 96.7% and 95.5%, respectively. Compared with Fe84Nb7B9 alloy (muti-phase microstructure), Fe80Ti8B12 alloy was composed of superfine crystalline (50~200nm) of single phase α-Fe;(3) the magnetic properties of Fe80Ti8B12 bulk alloy σ(subscript s=198.6emu/g, H=54.6Oe were more excellent than that of Fe84Nb7B9 bulk alloy σ(subscript s=162.9emu/g, H=105.1G, it was related to the different phases formed during hot-press sintering and the grain size of nanocrystalline powder.

结果表明：(1)20hMA后，Fe84Nb7B9和Fe80Ti8B12均形成了单相bcc纳米晶过饱和固溶体，相对前者，后者纳米晶组织热稳定性明显提高，过饱和固溶体相分解温度略有降低；(2)在30MPa/900℃／0.5h热压条件下，Fe84Nb7B9和Fe80Ti8B12块体合金相对密度分别为96.7%和95.5%，相对前者，后者由超细晶(50~200nm)单相α-Fe组成；(3)Fe80Ti8B12块体合金软磁性能σ(下标 s=198.6emu/g，H＝54.6Oe明显高於Fe84Nb7B9块体合金σ(下标 s=162.9emu/g，H＝105.1Oe，此与纳米晶粉末在热压烧结过程中所形成相的种类和晶粒尺寸大小有关。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

The harzburgite inclusions in western Liaoning could be relicts of partial melting of the mantle-derived peridotites; the amphibole-bearing spinel lherzolites could be representative of Phanerozoic accreted lithospheric mantle; the pyroxenite xenoliths with cumulate textures would be the cumulates of magma derived from asthenosphere in the upper mantle; the granulite and gneisses with crystalloblastic textures could be derived from the lower crust in the region.

辽西尖晶石方辉橄榄岩为地幔橄榄岩部分熔融的残留体；含角闪石的尖晶石二辉橄榄岩可能代表了显生宙新增生的岩石圈地幔；具有堆晶结构的辉石岩类包体为源于软流圈的母岩浆在上地幔顶部的堆积层；而具有变晶结构的麻粒岩和片麻岩类岩石包体则代表了本区下部地壳的物质组成，是岩浆上升过程中捕虏的深部地壳岩石包体。

By using such explorations testing ways as drilling, ultrasonic logging, pumping test, water analysis, microseismic survey and indoor earth mechanical experiments, the foundation bearing layer's features in soft rock iterbedded hard rock constituted by argillaceous dolomite rock, argillaceous limestone, pelitic mudstone and shave rock was determined. The foundation bearing layer's features and the slope design parameters of C and Φ in foundation pit was evaluated and excavated through local architectural experience.

采用钻探、超声波测井、抽水试验、水质分析、地微震测量、室内岩土力学室验等综合勘探测试方法，确定薄至中厚层状泥质白云岩、泥质灰岩夹泥岩、页岩组成的软质岩与硬质岩互层状岩体地基承载力特征值，结合地区的建筑经验合理的评价和充分挖掘软质岩与硬质岩互层状岩体地基承载力特征值和基坑边坡设计参数C、Φ。

These evidences lead to the conclusion that the Late Mesozoic volcanic activities in the study area are related to pulsative up-welling diapirs of asthenosphere, for which a natural passage is provided by the specific tectonic background confined by large-scale lithospheric faults in the study area.

根据上述特点，提出辽西晚中生代的火山岩活动可能与软流圈底辟体脉动式上涌有关，研究区被岩石圈断裂围限的特定构造背景为软流圈底辟体的上涌提供了天然通道。

The presence of austenite at any 10% would causeloss of strength of both steels about 10 kg/mm~2, however, their toughness mightbe actually improved by these softer austenites formed along the boundaries oflath martensite.

回火奥氏体的存在使强度下降，估计10％的奥氏体使强度损失约10kg/mm~2。但较软的回火奥氏体沿板条状马氏体边界形成改善钢的韧性，使冲击韧性a_K提高。

The government gives this to an agency like the NSF to spend on software development.

然後政府把这笔钱交给一个类似 NSF 的组织来负责软体的发展。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。