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At the same time, based on the extracted total RNA from the leaves of Zostera marina L., the cDNA clone could be obtained and the Na+/H+ antiporter gene could be studied by applying the techniques of RT-PCR and RACE. Its expression profile could be studied when applying the Northern technique.

同时在所成功提取出的大叶藻叶片组织的总RNA基础上,做出cDNA克隆,再通过RT-PCR和RACE技术等研究方法,来试图探究大叶藻的Na~+/H~+逆向转运蛋白基因,然后可结合Northern技术来研究其表达情况。

Secondly, the genomic DNA is extracted from Zostera marina L. and then amplified by PCR with two oligonucleotide primers synthesized to obtain the partial nucleotide sequences of the Na~+/H~+ antiporter gene. The Na~+/H~+ antiporter gene clone was obtained after a series of manipulation: purificatioin of PCR product, ligation and transformation of recombinant plasmids, blue-white selection and so on. DNA sequence analysis shows that its DNA sequence is highly homologous with the genomic DNA sequence of E.

其次,通过提取大叶藻基因组DNA,根据设计的简并性引物进行PCR扩增,纯化PCR产物,连接,重组质粒转化,蓝白斑筛选等操作,并测序,提交测定序列到NCBI进行BLASTN,结果表明:该序列与大肠杆菌的基因组序列相似性较高,而与其它物种的Na~+/H~+逆向转运蛋白基因没有相似性。

In the bacteriophage phi29, a virus-encoded RNA is required for the packaging of its genomic DNA. Six pRNAs form a hexamer through pRNA/pRNA interaction to drive the DNA translocation motor, and ATP as the energy resource.

在噬菌体phi29中,基因组DNA的包装需要由病毒基因组编码的pRNA参与,6个pRNA分子通过由pRNA分子间相互作用形成的六聚体来启动DNA转运马达,这个过程由ATP提供能量。

Antiport A membrane transport protein in which the energy liberated by the passive movement of H+ ions across the membrane is coupled to the active transport of another solute, e.g.

逆向转运:物质跨膜运动所需的能量来自膜两侧离子浓度梯度。

This willlead to acid productions increment,and intracellular acidifying.

糖酵解过程中必然产生乳酸,如果要维持细胞内的糖酵解状态,乳酸必须被转运出细胞外。

Objective To explore the possible molecular mechanism that rhein antagonizes the effect of TGFβ1 on glomerular mesangial cells. Methods Cultured C57/SJL mouse mesangial cells were used in the in vitro study.

目的 研究大黄酸对系膜细胞葡萄糖转运蛋白1(GLUT1)及葡萄糖摄入的影响,探讨大黄酸拮抗TGFβ1作用的可能机制。

Itamin D from the skin and diet is metabolized in the lier to 25-hydroxyitamin D (Figure 1), which is used to determine a patient's itamin D status1,2,3,4; 25-hydroxyitamin D is metabolized in the kidneys by the enzyme 25-hydroxyitamin D-1-hydroxylase (CYP27B1) to its actie form, 1,25-dihydroxyitamin D.1,2,3,4 The renal production of 1,25-dihydroxyitamin D is tightly regulated by plasma parathyroid hormone leels and serum calcium and phosphorus leels.1,2,3,4 Fibroblast growth factor 23, secreted from the bone, causes the sodium–phosphate cotransporter to be internalized by the cells of the kidney and small intestine and also suppresses 1,25-dihydroxyitamin D synthesis.5 The efficiency of the absorption of renal calcium and of intestinal calcium and phosphorus is increased in the presence of 1,25-dihydroxyitamin D (Figure 1).2,3,6 It also induces the expression of the enzyme 25-hydroxyitamin D-24-hydroxylase (CYP24), which catabolizes both 25-hydroxyitamin D and 1,25-dihydroxyitamin D into biologically inactie, water-soluble calcitroic acid.2,3,4

从皮肤和食物来的维生素D在肝中代谢为25-羟基维生素D(图1),被用来决定病人体内维生素D情况的1,2,3,4;25-羟基维生素D在肾中被25-羟基维生素D1羟化酶(CYP27B1)转变为有活性的1,25-二羟基维生素D 。1,2,3,4由肾产生1,25-二羟基维生素D是被血浆甲状旁腺激素和血清钙,磷水平紧密调节。1,2,3,4由骨分泌的成纤维细胞生长因子23使钠磷协同转运蛋白被肾和小肠细胞内化及抑制1,25-二羟维生素D合成。5 在1,25-二羟基维生素D作用下肾和小肠吸收钙及磷的效率增高(图1)。2,3,6 它也包括25-羟四- 24 -羟化酶的表达(CYP24),且将1,25二羟基维生素D和25羟基维生素D异化成无生物活性,水溶性的维生素D3-23羧酸。2,3,4

The action of dipyridamole conjugating on Panc-1 cell membrane on the intracellular concentration of 6-thiopurine was studied.

考察了作为核苷载体阻断剂的潘生丁对细胞膜上核苷载体转运6-巯基嘌呤的影响。

Cargo receipt issued by applicant and must be countersigned by beneficiary and/ or forwarder cargo receipt acceptable.

可以接受货物收据由申请人签发,而且必须要受益人和/或转运货物收据。

Based the alterations in both morphological characters and the corresponding to plant exogenous hormones for transformant, we deduce that BcpLH may be a upstream controlling gene. It is rather to think that its function is related to the production or transportation of plant hormones than to say it corresponds to forming of leaf curding.

鉴于转化体在整个生育期都有表型变化,并且对激素有异常的反应,我们认为,BcpLH可能是上游调控基因,其功能与其说与结球相关不如说可能与激素产生或转运有关。

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Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

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