转移酶

The advantage of supercritical carbon dioxide used as an environmental medium in polymerization and reactions in supercritical carbon dioxide are described. It includes homogeneous free radical polymerizations, precipitation free radical polymerizations, dispersion free radical polymerizations, emulsion and inverse emulsion free radical polymerizations, cationic polymerizations, ring-opening polymerization, melt-phase condensation polymerizations, sol-gel polymerizations, polymer blend synthesis, catalytic chain transfer polymerizations, oxidative coupling polymerization, nitroxide-mediated radical dispersion, atom-transfer radical polymerization, reversible addition-fragmentation chain transfer polymerization, electrochemical polymerization, simultaneous one-pot combination of enzymatic and chemical polymerization, copolymerization of carbon dioxide used as monomer.

评述了ScCO2作为聚合反应介质的优点，以及在ScCO2中可进行的聚合反应类型，包括均相聚合、沉淀聚合、分散聚合、乳液及反相乳液聚合、阳离子聚合、开环聚合、熔融态缩聚、溶液-凝胶聚合、聚合物混合合成、链催化转移聚合、氧化-偶合反应聚合、氮氧自由基可控活性聚合、原子转移自由基聚合、可逆加成-断裂链转移、电化学聚合、原子转移聚合与酶催化开环聚合两种活性聚合在ScCO2中同时聚合、二氧化碳作为原料共聚合等。

D-galactoside galactohydrolase (EC3.2.1.23) is usually named lactase which can hydrolyze lactose into galactose and glucose, and also can transfer galactoside.

半乳糖苷酶(B-D-半乳糖苷半乳糖水解酶，β-D-galactoside galactohydrolase，EC3.2.1.23)通常被称为乳糖酶，能将乳糖水解为半乳糖和葡萄糖，并具有半乳糖苷的转移作用。

The clathrin triskelions assenble into a basket-like convex framework that causes the membrane to invaginate clathrin-coated vesicles migrate into the cell where the clathrin coats are lost before delivering their contents to the lysosomes.

网格蛋白三脚复合体装配成篮状凸起懂得框架，使得膜在该处内陷，最终出芽而形成小泡。而在胞吞作用中，这些网格蛋白包被的小泡转移到细胞中，此处网格蛋白外被在小泡的内含物转移到溶酶体之前即行消失。

We localized MMP9 expression in ceil type of the human prostate by using the method of primary cell cultures combined with RT-PCR.

为了深入了解前列腺肿瘤细胞在浸润和转移过程中，与肿瘤侵袭、转移能力相关的异常基因的表达，我们采用单管半定量RT-PCR、酶谱电泳及免疫印迹技术对前列腺癌组织中的MMP-2、MMP-9，TIMP-1、TIMP-2的异常表达情况，在基因及蛋白表达水平上进行了定量分析。

Objective The expression of iNOS bFGF CyclinD1 erbB-2 were studied by immunohistochemistry to investigate the relationships between the expression of iNOS and proliferative index of breast carcinoma such as bFGF CyclinD1 and erbB-2.The relationship between expression of iNOS and ages menstruous status tumor size histological stage TNM stage venous invasion ER PR lymph-node metastasis distant metastasis and survival rate in 5 years.

目的 研究诱导型一氧化氮合酶(Inducible Nitric Oxide，iNOS)在乳腺癌中的表达及与乳腺癌增殖指标碱性成纤维细胞生长因子、细胞周期素D_1(CyclinD_1)、癌蛋白(erbB-2)的关系，同时研究iNOS与年龄、月经状况、肿瘤大小、肿瘤学分级、TNM分期、脉管侵袭、ER、PR、淋巴结转移、远处转移、预后的关系。

METHODS: Rat bilateral olfactory bulbs and olfactory mucosa at 1/3 nasal septum were obtained, sliced, digested in trypsin, and made into monoplast suspension. At 1×109/L, cells were incubated in uncoated 25 cm2 culture flask. At 18-20 hours, cell suspension was moved into another uncoated 25 cm2 culture flask (the first differential adhesion). At 24 hours, cell suspension was moved into a poly-D-lysine-coated 25 cm2 culture flask or poly-D-lysine-coated 6-well culture plate (the second differential adhesion). At 48 hours, parenchyma cells were removed after a half of medium was changed.

完整取大鼠双侧嗅球及剪取鼻中隔后1/3嗅黏膜，剪碎后胰酶消化，制成单细胞悬液，按1×109 L-1密度接种于未包被的25 cm2玻璃培养瓶中，18~20 h后将细胞悬液转移至另一未包被的25 cm 2玻璃培养瓶中（第1次差速贴壁），24 h后再将细胞悬液转移至经多聚右旋赖氨酸包被的25 cm2塑料培养瓶或经多聚右旋赖氨酸包被的6孔培养板中（第2次差速贴壁），接种培养48 h后半量换液以去除杂细胞。

We study the express of heparinase,matrix metalloproteinase MMP-2,MMP-14 and cadherin in the gastric cancer tissue and perigastric normal mucosal tissue,and make a statistics analysis between heparinase, matrix metalloproteinase MMP-2,MMP-14,E-cadherin and gastric cancer biological behavior.We hope to knom the possibility that heparinase,matrix metalloproteinase MMP-2,MMP-14,E-cadherin are regared as clinical screen index of the gastric cancer recrudescence or metastasis.

本文就肝素酶、基质金属蛋白酶MMP-2、MMP-14、钙粘蛋白在胃癌组织及胃周正常粘膜的表达进行了研究，并就肝素酶、基质金属蛋白酶MMP-2、MMP-14、钙粘蛋白与胃癌的生物学行为进行了统计学分析，以期了解肝素酶、基质金属蛋白酶MMP-2、MMP-14、钙粘蛋白作为胃癌复发或转移的临床筛选指标的可能性。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

The one carbon unit transfer reaction involving in the tetrahydrofolate coenzymes has caused great interest which is very important in the living system and has played an important role in the designing and syntheses compounds (especially the anti-cancer and LCD materials). In our study it was found that benzimidazolium salt has the ability to transfer one or two one-carbon unit and is a kind of new tetrahydrofolate coenzymes model. This research provides new method for biomimetic synthesis.

在非酶催化条件下，不仅实现了与生物体内相类似的一碳单元转移反应，而且可以转移具有不同官能团的取代&一碳单元&，使这种一碳单元转移反应传递的功能化一碳单元更加多样化，这方面强劲的研究工作进展，将为异常活跃的合成化学提供更多常规合成方法难以实现或实现不理想的仿生合成新方法。

Huang-hua' pear fruit was a typical climacteric fruit after harvest, and the fruit senescence-related factors, such as LOX activity, 02" content, AOS activity, ACC synthase activity, ACC content, ACC oxidase activity and ethylene production, changed with peaks at 20 "C. The activities of LOX, AOS, ACC synthase, ACC oxidase and the accumulation of O2 ", ACC content and ethylene synthesis were inhibited when stored at 1癈. And the severity of flesh floss and fruit decay was lightened. Low temperature combined with polyethylene film bag packing (PEF1 and PEF2) could further decrease the rate of weight loss, flesh floss, and fruit decay and keep preferable freshness. When PEF1 and PEF2 packing fruits were transferred to shelf life at 20 "C after storage at 1癈. The activities of LOX, AOS, ACC synthase, ACC oxidase could be suppressed and the accumulation of 02 , ACC content and ethylene production were slowed down to a certain extent, especially during the shelf life after 60 d storage. PEF1 and PEF2 packing fruit could maintain higher firmness as well, and the effects became more distinct along with the storage period, there existed no significant difference between PEF1 and PEF2 treatments.

试验结果表明，在20℃下，LOX活性、O2-水平、AOS活性、ACC合成酶活性、ACC含量、ACC氧化酶活性及乙烯的生成，均呈峰形变化；1℃处理可抑制LOX、AOS、ACC合成酶、ACC氧化酶的活性和O2-、ACC的积累，抑制贮藏果实的乙烯合成，减轻果肉发绵和果实腐烂。1℃下贮藏的果实，若结合不同聚乙烯薄膜袋(PEF1和PEF2)小包装，可以进一步降低果肉发绵率和果实腐烂率，保持果实新鲜度；当果实经过一段时间贮藏后，转移到20℃下，在货架期间，PEF1和PEF2对果实成熟衰老各个相关因子均有不同程度的抑制效应，主要表现在贮藏60d后的货架期间，同时维持较高的果实硬度，对硬度的影响随着贮藏时间的延长愈加明显，PEF1和PEF2处理效果之间无显著差异。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。