转移酶

RESULTS: Image analysis of two-dimensional map revealed 72 differentially expressed proteins between MHCC97-H and MHCC97-L cells, and 9 protein peaks (including M2 pyruvate kinase,α-subunit of ATP synthase, heat shock protein 60, Toll-like receptor 9, flavoprotein oxygenase, pro-calreticulin, manganese superoxide dismutase, nm23-H1, and G-protein-coupled receptor kinase 5) were further identified by tryptic digestion, peptide mass fingerprinting and mass spectrometry.

结果： 2D图谱显示共有72个差异蛋白条带，共鉴定出了9个差异蛋白，分别为M2型丙酮酸激酶、ATP合成酶α亚单位、热休克蛋白60、Toll样受体9、含黄素单加氧酶、钙网硬蛋白前体、锰超氧化物岐化酶、nm23-H1、G-蛋白偶连受体激酶5；其中4个蛋白在高转移细胞株MHCC97-H中表达升高， 5个蛋白在低转移细胞株MHCC97-L表达升高。

2 A kind of enzyme and mediator modified electrode is devised by immobilyzing both N-methyl phenazine sulfate and glucose oxidase on the surface of carbon glassy electrode (GOD-NMP-GC electrode), which successfully realizes the electron transfer process between glucose oxidase and carbon glassy electrode.

将NMP-GC电极与葡萄糖氧化酶结合，制作了GOD-NMP-GC电极，该电极可快速实现电极=NMP介体=GOD活性中心=葡萄糖之间的电子长程转移，并且用循环伏安法对酶催化反应进行了研究；用含丰富乳酸脱氢酶的乳酸杆菌与NMP-GC电极相结合，制作了介体微生物电极，并检测到GC电极=NMP=LDH活性中心=乳酸之间的非均相电子转移。

METHODS: MyoD cDNA fragments were extracted from plasmids pEMSV-MyoD with polymerase chain reaction, and PCR was used to clone the whole-length gene of MyoD. After adding CACC sequence at 5' end, MyoD gene was cloned by orient topology into transfer ventor, pENTR/D-TOPO. Objective gene was transferred into adenoviral expression vector DNA via pENTR/D-TOPO vector. The recombinant adenoviral vectors transfected into HEK293A cells by using lipofectamine were packaged and amplified.

从pEMSV-MyoD质粒上用聚合酶链反应法扩增出MyoD cDNA片段，再通过聚合酶链反应使MyoD基因加上CACC序列接头，经过定向拓扑克隆使目的基因连接到转移载体上，再通过LR酶促反应，将目的基因转移到腺病毒表达载体DNA上，获得MyoD基因重组的腺病毒DNA，用脂质体转染法转染HEK293A细胞，包装扩增出MyoD基因重组的腺病毒。

The applications of SECM in interfacial electrochemistry were discussed in detail, especially. These applications include:(1) SECM were used to provide a means of characterising thin films, modified electrodes and conductive polymers in terms of structure and mechanism.(2) The catalytic activity of immobilised redox enzymes were studied by SECM.(3) Employed SECM to study of ions transfer, electron transfer and neutral molecules transfer at the interface.

主要包括：(1)研究化学修饰电极界面或导电聚合物薄膜界面，获得界面的结构和多相反应的机理等相关信息；(2)研究固定化酶，获得酶活性等信息；(3)研究界面上的电子转移和离子转移以及中性分子的转移；(4)采用纳米级的探头，研究单分子的电化学反应；(5)结合其它相关电化学技术，实现对单细胞活性的检测。

MethodsHepatocyte injury model was induced by CCl4 and the injured hepatocytes were incubated with rat serum obtained after oral administration of CWC. The hepatocyte proliferation rate and alanine aminotransferase concentration in cell culture medium were determined. 446 patients with chronic hepatitis were divided into experimental group and control group treated by Hu Gan Tablets, HGT） randomly. With physical sign, symptom and hepatic function as main indices, the therapeutic effect of CWC on patients with chronic hepatitis was evaluated.

方法以SD大鼠为供体制备复方五仁醇胶囊含药血清，CCl4诱导大鼠原代培养肝细胞损伤模型，采用血清药理学方法考察含药血清对损伤肝细胞增值和培养上清液中丙氨酸氨基转移酶的影响；将446例慢性乙型肝炎患者随机分成治疗组和对照组，以临床症状、体征、肝功能为主要指标，考察复方五仁醇胶囊的治疗效果。

The hepatocyte proliferation rate and alanine aminotransferase concentration in cell culture medium were determined. 446 patients with chronic hepatitis were divided into experimental group and control group treated by Hu Gan Tablets, HGT） randomly. With physical sign, symptom and hepatic function as main indices, the therapeutic effect of CWC on patients with chronic hepatitis was evaluated.

方法以SD大鼠为供体制备复方五仁醇胶囊含药血清，CCl4诱导大鼠原代培养肝细胞损伤模型，采用血清药理学方法考察含药血清对损伤肝细胞增值和培养上清液中丙氨酸氨基转移酶的影响；将446例慢性乙型肝炎患者随机分成治疗组和对照组，以临床症状、体征、肝功能为主要指标，考察复方五仁醇胶囊的治疗效果。

Abstract—A yeast plasmid was constructed to contain a hybrid GAL–CYC promoter, the NPTII neomycin phosphotransferase gene, and the FRT sequence between them.

摘要，酵母质粒构建了含有混合放射公益少年团启动，新霉素的NPTII 磷酸转移酶基因，并首次登记税它们之间的序列。

The items of investigation on physical and mental health included general information, rational symptom, function of autonomic nerve and sensory nerve, blood routine, conjugated bilirubin , serum protein , glutathione-s-transferase, malondialdehyde, reproductive functions, state of mental health.

工人身心健康调查项目包括一般情况、自觉症状、自主神经和感觉神经功能、血常规、血中结合胆红素、血清蛋白、谷胱苷肽-S-转移酶和丙二醛、女工生殖功能和心理健康状况。

Objective:To study the relations between β-1,4-GalT-I and the aging of Schwann cells.

目的：探讨β-1，4-半乳糖基转移酶I与雪旺细胞衰老的相关性。

Objective: The aim of this study was to investigate the effects of ginsenoside Rg3 and ginsenoside Rg3 combined with cisplatinon the growth of nude mice cervical cancer, and to explore the possible mechanism of ginsenoside Rg3 and ginsenoside Rg3 combined with cisplatinin cells and molecules levels. The growth of human cervical cancer Hela cell, apoptosis of tumor cells , expression of CD31and PeNt were observed with optic microscope, radio-immunity immunological histo chemistry, terminal deoxynucleotidy transferase-mediated dUTP nick end labeling, and immunohistochemical staining. The purpose of this study is to provide a new way of medication to eure cervical cancer in clinic.

目的：本实验通过建立子宫颈癌裸鼠病理模型后，以此为研究对象，运用光镜、免疫组化及末端脱氧核苷酸转移酶介导的dUTP缺口末端标记技术，观察人参皂甙Rg3[20—Ginsenoside Rg3]和顺铂单独及二者联合应用对人宫颈癌Hela细胞裸鼠移植瘤生长的影响，重点研究肿瘤的生长、肿瘤细胞的凋亡及PCNA、CD31的表达情况，以期从细胞及分子水平揭示人参皂甙Rg3和顺铂单独及二者联合应用对人宫颈癌Hela细胞的作用机制，为临床上药物治疗与预防宫颈癌寻找更加完善的途径。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。