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转移酶

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In order to express he Opening Reading Frame fragment of Banana bunchy top virus Hainan isolateDNA2 correctly ,a pair of primers were designed in accordance with the reported BBTV-HN DNA2 gene sequence.

本文根据已报道的香蕉束顶病毒海南分离物(Banana bunchy top virus BBTV-HN)BBTV DNA2的基因序列,设计一对引物,以海南地区表现束顶病症状的香蕉组织总DNA为模板,利用PCR方法克隆了DNA2编码区,将其插入到原核表达载体pGEX-6p-1谷胱苷肽-S-转移酶基因下游。

Here we show that signal peptides have a new role beyond targeting: they are essential allosteric activators of the translocase.

在这里我们展示了信号肽除了靶向作用外的新的作用:他们是转移酶的重要的变构激活剂。

A role for signal peptides and targeting sequences as allosteric activators may be universal in protein translocases.

这种信号肽在对转移酶的变构激活中的作用可能是普遍的。

The reaction is catalysed by a transaminase or an amino transferase.

反应是由转氨酶或氨基转移酶?催化的。

The recombinant proteins were purified with affinity columns by the 6 histidines tag or Glutathione-S-Transferase they were fused to.

并根据所表达融合蛋白的6个组氨酸或谷胱甘肽-S-转移酶标记进行亲和纯化。

Keywords: NMT; Randomly phage display library; Affinity binding; Screening of inhibitor peptides; Molecule-screening model

中文关键词:豆蔻酰转移酶;噬菌体展示随机肽库;亲和结合;抑制肽筛选;分子筛选模型

Thus, Dnmt 3a and Dnmt 3b were proposed to be the long-sought de novo methyltransferases.

由此推测它们很可能就是长期以来努力寻找的重新甲基转移酶

Infant ; newborn ; jaundice ; uridine 5'-diphosphate glucuronosyl transferase ; gene mutation

婴儿;新生;黄疸;尿苷二磷酸葡萄糖醛酸转移酶;基因突变

Objective To explore the association of Gly71Arg mutation in gene of bilirubin uridine 5'-diphosphate-glucuronosyltransferase(UGT1A1)and neonatal jaundice in Beijing city Han population.

目的探讨胆红素-尿苷二磷酸葡萄糖醛酸转移酶(UGT1A1)基因Gly71Arg变异与北京地区汉族新生儿黄疸发病的关系。

In contrast, deletion of the propeptide yields mature but inactive NK, the inclusion bodies of NK or denatured rNK couldn't be renatured in any condition, but with the aid of exogenously purified propeptide or Ser221Ala proNK, the inactive mature protein could be renatured by an intermolecular pathway in vitro.

此外,我们利用融合基因表达系统在大肠杆菌中高效表达了谷胱甘肽S-转移酶/NK和硫氧还蛋白/NK两种融合蛋白,经体外复性、纯化,均获得活性蛋白。以上结果显示,pro肽在体内体外,通过分子内或分子间的方式均能帮助NK分子的折叠与再折叠,作为一种分子内的分子伴侣,pro肽为NK分子正确折叠所必需。

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