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In bud stage of mustards ecotype Xuelihong and Yuanyejie were transformed with in planta vacuum infiltration by an Agrobacterium tumefaciens strain GV3101 carrying hy-gromycin resistance gene. Effects of different factors on transformation frequency with in planta vacuum infiltration were studied.

以蕾期的芥菜生态型雪里蕻和圆叶芥为试材,在携带潮霉素磷酸转移酶标记基因的根癌农杆菌菌株GV3101的介导下,对影响芥菜植株原位真空渗入遗传转化效果的真空渗入时间、根癌农杆菌悬浮孢子的浓度、渗入培养基、感染方式以及根癌农杆菌菌株等进行研究。

The polarographic complex adsorptive wave was firstly used to detect DNA specific sequences of the phosphinothricin acetyltransferase gene.

应用极谱络合吸附波方法检测了草丁膦乙酰转移酶基因的DNA特定序列。

Objective To observe the changes of praxiology and choline acetyltransferase in cerebral cortex, basal forebrain and hippocampi CA1 of rats after ovariectomized and transected hippocampal fimbriafornix and protective effect of premarin on brain.

目的 观察去卵巢痴呆大鼠行为学及额叶皮质、基底前脑、海马CA1区胆碱乙酰转移酶的变化及倍美力对其影响,探讨倍美力的脑保护机制。

Methods: A brain concussion was produced in rats using a simple pendulum device model. One control (n=12) and six experimental groups were used(n=12 in each group). The cerebral concussion groups were assigned to sacrifice times ranging from 1 day to 24days after injury.A Morris Water Maze memory paradigm was used to assess learning and memory function. Pycnosis degeneration or necrosis neurons in the locus cerebral cortex, dorsal hippocampus, dentate fornix and brainstem reticular formation were observed and counted after brain concussion. Cholinergic neurons in the basal forebrain and brainstem reticular formation were identified and quantitated using choline acetyltransferase.

应用自制单摆式闭合性机械打击装置建立脑震荡大鼠模型;成年SD大鼠随机分为1个对照组(n=12)和6个脑震荡组(即伤后1、2、4、8、16、24d,n=12);应用Morris水迷宫(Morris Water Maze,MWM)实验评价大鼠的学习记忆功能;用光镜对大鼠的上述脑区的固缩变性坏死细胞进行观察和计数;用免疫组化方法检测基底前脑和脑干网状结构的胆碱乙酰转移酶(cholin acetyltransferase,ChAT)阳性细胞的表达。

The changed physiological parameters in the body of ladybird in every season caused these differences. The body weight including fresh and dry weight, content of fat and glycogen, activities of glutathione S-transferase in the body of H. axyridis all decreased gradually in overwintering. All these indices began to recircle after hibernation and would be up to the optimum status when they entered dormancy.

造成这种差异的原因是不同时期异色瓢虫体内的一些生理指标不一样,在越冬期异色瓢虫体重、脂肪和总糖含量逐渐降低,谷胱甘肽S-转移酶的活性也逐渐下降,出蛰后各项指标又开始回升,直至进入越冬时达到最佳状态。

Then a high-efficiency genetic transformation system was established, by which two transgenic Roselle cell lines that stably express the activity of neomycin phosphotransferase Ⅱ were obtained.

利用这一转化体系获得了2个稳定表达新霉素磷酸转移酶活性的玫瑰茄转化细胞系。

Moreover, the researchers believe that in soybean, cotton, Brassica and Solanaceae plants and the development of forest tree species, AtWBC19 mycophenolate may be an alternative new phosphotransferase.

而且,研究人员认为在大豆、棉花、芸苔和茄科植物及森林树种的开发中,AtWBC19也许可替代新霉磷酸转移酶

Methods The model of subacutely aging rats were made by injecting Dgal into the rats, abdominal cavity continually. After lavaging HSWY, the apoptosis rate and the levels of Fas and FasL mRNA were measured by TUNEL and RTPCR; the expressions of Fas/Fasl protein were detected by SP immunocytochemistry.

采用D半乳糖连续腹腔注射致亚急性衰老大鼠模型,同时以何首乌饮灌胃作为延缓衰老组;造模后应用脱氧核糖核苷酸末端转移酶介导的缺口末端标记法检测各组大鼠卵巢细胞凋亡情况;测定各组Fas和FasL的信使核糖核酸水平;SP免疫组化法检测卵巢细胞Fas和FasL蛋白的表达情况。

It can inhibit the transmethylation activity through conjugating with DNMT1 and forming a covalent compound when conjugated with replicating DNA molecule. Then the formation of hypomethylated daughter chain makes the demethylation function ture.

体外实验已经证实DNA甲基转移酶抑制剂5Aza-dc通过去甲基化作用可使多种含有CpG岛的高甲基化抑癌基因重新表达,从而恢复抑癌功能,这一研究结果对于肿瘤的基因治疗提供了一个新的思路。

In contrast to lung sequestration of neutrophil count,there were no transpulmonary gradient of MBE between left and right atrium after aortic declamping.

MBE或MBE总活性与术后炎性反应标记物如C反应蛋白及组织功能标记物如乳酸、肌酸磷酸激酶、丙氨酸氨基转移酶之间均无相关关系。

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