转移酶

Three contig cDNA sequences of 2273,2282 and 2288 bp were determined,which encoded a predicted protein of 596 amino acid residues with calculated mass of 67.304,67.325 and 67.304 kDa.The gene products were designated as Fragaria×ananassa DRMa,Fragaria×ananassa DRMb and Fragaria×ananassa DRMc,respectively.

通过5′和3′RACE试验从草莓中还分离获得了3条DRM类DNA甲基转移酶基因cDNA全长序列，长度分别为2273，2282和2288 bp，分子量依次为67.304，67.325和67.304 kDa.3条基因序列均编码596个氨基酸，分别命名为FaDRMa、FaDRMb、FaDRMc。

In this study, an attempt is made to account for the observed difference in the susceptibility of Gossypium hirsutum L.(cv. BD-18 and Simian 3, resistant and susceptible to V. dahliae, respectively) to toxin of Verticillium dahliae, by looking for differences in the timing and level of production of NO and H_2O_2, and their effects on the expression of GST gene.

本实验以棉花抗病品种BD-18和感病品种泗棉三号的悬浮细胞为材料，研究了棉花细胞在黄萎病菌毒素处理后H_2O_2和NO的变化，并用半定量RT-PCR的方法检测毒素、H_2O_2和NO对棉花谷胱苷肽-S-转移酶基因转录水平的影响。

Results Digestive disorder, elevated alanine transaminase and rash were the most common side effects of antiretrovirals, fatigue, loss of hair, sleep disorder, fat maldistribution, peripheral neuropathy and hypocytosis were also commonly seen, yet pancreatitis, color change of skin and lactic acidosis were seldom seen.

结果 消化道症状、血清丙氨酸氨基转移酶升高、皮疹为最常见的艾滋病抗病毒药物不良反应，疲倦、脱发、失眠、脂肪分布异常、四肢肢端麻木、血细胞减少为较常见的不良反应，而胰腺炎、皮肤色素沉着和乳酸性酸中毒则为较少见的艾滋病抗病毒药物不良反应。

Tolcapone could greatly improve PD patients'motor function,especially in hypokinesia,muscle rigidity,and which could also improve patient's cognition ability.

儿茶酚 O位甲基转移酶抑制剂托卡朋能够改善PD的运动功能，尤其是运动减少和肌僵直，同时能改善患者的认知功能。

Although the deelopment of relatiely non-toxic immunosuppressie or tolerance-inducing regimens will be required to justify clinical trials using pig organs, recent adances in our understanding of the biology of xenograft rejection and zoonotic infections, and the generation of alpha1,3-galactosyltransferase-deficient pigs hae moed this approach closer to clinical application.

尽管用猪的器官去验证临床试验需要相对无毒的免疫抑制剂或致耐的方法，但随着近来我们对异种移植排斥生物学及动物传染病感染的深入理解，以及α1，3 半乳糖转移酶缺陷猪的产生，异种移植更接近临床应用。

Pretreated with the extract from Morchella esculenta for 16 days,mice modles of acute hepatic injury were established by the treatment of carbon tetrachloride (CCl4).After 24 hours,the protective effects of the extract from Morchella esculenta in acute hepatic injury were observed through determing the activities of alanine aminotransferase,, Maleic Dialdehyde ,and the contents of proteins in serum.

预防给药16d后，采用CCl4复制小鼠急性肝损伤模型，24h后，眼眶取血，测定血清中丙氨酸氨基转移酶、超氧化物歧化酶、丙二醛以及血清蛋白的变化，观察羊肚菌水提物对急性肝损伤的保护作用。

A acute partial obstructive hepatocholangitis model by selective ligation and injection of E coli into left hepatic bile duct was successfully founded in rat. Using parameters including mortality, mitochondrial glutamic oxalacetic transaminase and ornithine carbamoytransferase activity, pathological observation and blood culture of bacteria, we evaluated the model.

作者采用选择性左肝管穿刺注菌法成功地建立了Wistar大鼠急性部分肝胆管梗阻感染的脓毒症模型，并从胆管系统形态学、累计病死率、血清线粒体谷草转氨酶和鸟氨酸氨基转移酶活性、多器官大体病理观察以及血液细菌培养几方面对该模型的科学性、重复性进行了评估。

TyPe II collagen induced arthritisln the rat ank1e joint andoVathumin as antigen induced arthritis WA in the rabbit knee joint wereestab1ish2 Qualitative evaluation of me in skin, muscle, synovium, cedilagearound joint and blood was performed by OMA3 The CIA rats were treated on day 7 after hind paw swelling and erythemaAnimals were injected intravenously with ase at a dose of 10mg/kg,tWenty minuots 1ater, one ankle of the rats random1y assigned was exPosedlaser irradiation at l00J/cm fOr l000 seconds, and another ankle wasM grouP wihout laser The other two groups is unmanipulatedcontrol group and untreated CIA group Bimaleolar ankle widthmeasuremellts were taken in all animals every tWo days using amicrometer The histopathology of the ank1e Joint was assessed at day 21after disease onset4 The pro1iferating cell nuclear antigen WCNA of CIA treated by PDT andthe HMME group without laser was doterdrined by immunohistochemiStry5 The AfA rabbits were treated on day 7 after knee swelling and erythemaThe theraPy invo1ved lntravenous injection of l0mg/kg HMME, fOl1owedby 20 minues period in dim light, and transdermal light treatment with\l00 J/cm2 fOr l000 seconds The inner sides of the treated Anees wereirradiated at first, and then the outer side did 24 hours later, the synovialtissue of the Anees joint were removed and in situ cel1 aPoptosis wasdetCCted With tednal deoxync1eotidyl transferase-mediated dUTP nickend labelingR6suIt8:l The pathologic changes of CIA and AIA include subsynovial inflammation,opovial hyPerplasia, pannus formation, cartilage and bone destructionresemble RA.2 The studies demonstrated that there are different uptake of HMME withinskin, muscle, synovium, cartilage and b1ood, and the synovium cou1draPidly uPtake more ase than skin and cartilage at the firSt 30 minuesaller intravenous injection of HMME3 The bimaleolar anke width had no different among PDT treated group,H group withollt 1aser and untreated CIA group But hlstologicalevaluation showed statiStical1y significallt reductions in synovialhyperplasia, pannus formation and cart1lage reosion, bone destruction andtotal score in PDT treated group4 Image analysis showed that the ratlo bforeen the areas of the coufltedobect to that of the entire area in PDTtreated grOup is lower than that in conirol group, but the integrated oPticaldensity had no different between the two groups5 Imape analysis showed that the ratio between the area of the countedobject to that of the e

治疗组在大鼠出现踝关节红肿后1周，炎症达到高峰时进行PDT治疗。随机治疗大鼠一侧的踝关节，另。2。一一侧作单纯HMME 对照。治疗方法是大鼠麻醉后尾静脉注入 HMME10ngkg，20分钟后踝关节照光，激光波长627.sum，功率密度 100mwcm'，照射时间1000秒，能量密度100）／。治疗后避光喂养72 小时。隔日一次测量大鼠的踝关节左右横径，治疗后两周取关节进行病理d 观察。 4。大鼠CIA模型用上述方法进行PDT治疗后，治疗组和单纯HMME 组用兔疫组化SP法检测石蜡切片的核增殖抗原。 5。兔AIA模型在关节炎出现第七天进行PDT治疗，随机治疗一侧膝关节，另一侧作自身对照。兔耳静脉注入I'arrainrelomg／Kg，20分钟后，膝关节用金蒸气激光照射，激光能量密度100）儿旷。24 ／J'时后取膝关节滑膜作病理检查，并用脱氧核昔酸末端转移酶介导的缺口末端标记法原位检测凋亡细胞。结果： 1。模型观察：CIA大鼠炎症高峰期滑膜下炎细胞浸润明显，滑膜细胞明显增殖，炎症达到高峰后二周，血管缀形成，并侵蚀和破坏软骨和骨， CIA模型病理改变与人类RA相似。兔AIA模型膝关节滑膜病理可见滑膜细胞增生，滑膜下炎细胞浸润，也与人类RA滑膜改变相似。 2。关节周围组织中光敏剂含量的测定结果表明，各组织对HMME 的吸收速度和吸收量不同，荧光值一时间曲线不同，滑膜组织比皮肤和软骨对 HMME的吸收多，在 2 0分钟时即有明显差异。 3.PDT对CIA模型的治疗结果表明：PDT治疗后关节炎组、单纯 HMME组和治疗组踝关节左右横径统计学检验差异没有显著性，但病理评分PDT治疗组滑膜增生、血管资形成及软骨破坏、骨破坏和总分比关节炎对照组和HMME对照组好，统计学检验差异有显著性。。3＿军医进修学院硕士学位论文中文摘要 4.PDT治疗组PCNA阳性细胞较对照组少，图像分析结果表明面密度（阳性染色的面积总和与统计视野面积的比值）治疗组小于对照组，统计学检验差异有显著性。。 5.PDT治疗组凋亡阳性细胞较对照组明显增多，图像分析结果单位视野内阳性细胞数和面密度PDT治疗组高于对照组，统计学检验差异有显著性。凋亡细胞核直径PDT治疗组较小，与对照组相比，统计学检验差异有显著性。结论：二。CIA、AIA的病理改变类似人类RA，可作为研究RA病因、发病机制、检查及治疗方法的模型。 2。各组织对HMME的吸收速度和吸收量不同，滑膜组织比皮。

The proportion of GGT complexed with low density lipoproteins plus very-low density lipoproteins in total GGT in serum is determinated with ratio colorimetery,sodium phosphotungstate and MgCl2 as precipitation.26 patients with liver cancer,18 with liver cirrhosis,22 with chronic hepatitis,14 with the other malignant tumors,16 with non-hepatobiliary disease of high GGT and 50 healthy subjects have been evaluated.

本文以磷钨酸－氯化镁作沉淀剂，速率法测定血清低密度脂蛋白，极低密度脂蛋白结合的r－谷氨酰转移酶（LDL－VLDL－GGT）占总GGT的百分比。对临床26例肝癌、18例肝硬化、22例慢性肝炎、14例其他恶性肿瘤、16例GGT升高的非肝脏疾病及50例健康人进行了检测，结果表明：肝癌组明显高于其他各组，差异显著（P＜0.01）。肝脏疾病升高幅度：肝癌＞肝硬化＞慢性肝炎，且每两者间有显著差异（P＜0.01）。

The phytopathogenic fungus Fusarium graminearum has been transformedusing a plasmid (pAN7-1) containing the Escherichia coli hygromycinphosphotransferase gene. Stable hygromycin-resistant transformant coloniesappeared at frequencies between 2 and 10 per μg plasmid DNA.

将含有大肠杆菌中潮霉素B磷酸转移酶控制基因的质粒pAN7-1转化入小麦赤霉病菌野生菌株中，结果表明，pAN7-1的转化效率为2-10个/μg质粒DNA。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新