转移酶

So it is assumed that the E-box might be associated with the enhancement of transcription activity.Here, the LTRs of WH17 ,DLA-25,DLA-118 and LTR with single mutation in the E-box motif were separately cloned into pCAT-Basic vector resulting in a series of recombinant plasmids containing CAT reporter under the control of different versions of LTRs.

通过对EIAV分离株WH17的LTR与L株、DA和DLA株的LTR序列进行比较，发现其在在U3-R结合处多一个E-box基序，推测该基序的变化可能会起到促进转录作用，为此将EIAV强毒株(DLA-25)、驴白细胞弱毒株(DLA-118)、EIAV分离株WH17以及U3-R结合处的E-box基序点突变的LTR分别克隆到pCAT3-basic质粒中的报告基因氯霉素乙酰转移酶的上游，获得一系列受不同LTR控制的CAT表达质粒。

In addition, Dnmt2 also displays weak DNA methyltransferase catalytic activity, but newly founded function is to methylate cytosine 38 in the anticodon loop of tRNA.

此外，Dnmt2也具有弱的DNA甲基转移酶活性，近年来发现它可以甲基化tRNAAsp反密码子环处38C。

This promoter was used to construct an induction expression vector for monocotyledon (pC1300WDREB) with transcription factor gene of stress resistance and hygromycin phosphostransferas gene.

用该启动子与抗逆相关转录因子基因和潮霉素磷酸转移酶标记基因一起，构建成单子叶植物诱导表达载体pC1300WDREB。

Acetylator phenotype ; 2－naphthylamine ; bladder cancer ; genetic risk factors

乙酰基转移酶表型； 2－萘胺；膀胱癌；遗传易感因素

Alzheimer disease: A primary degenerative cerebral disease of unknown etiology with characteristic neuropathologcal and neurochemical features, including cortical atrophy with neurofibrillary tangles and neuritic argentophilic plaques, and a marked reduction on the enzyme choline acetyltransferase, in acetylcholine itself, and in other neurotransmitters and neuromodulators.

阿尔茨海默病：一种病因未明的原发退行性大脑疾病，具有特征性的神经病理和神经生化改变，包括伴有神经原纤维缠结和神经炎性嗜银斑的皮层萎缩，胆碱乙酰转移酶、乙酰胆碱本身、以及其它神经递质和神经调质的明显减少。

Nicotinamide phosphoribosyltransferase is a cytokine with highly conserved structure. It received little notice during the first ten years.

烟酰胺磷酸核糖转移酶是一种结构高度保守的细胞因子，在最初克隆后近十年中Nampt并未引起关注。

And on the other hand, utilize the genetic technology to overproduce levansucrase and further, use the method of immobile enzyme, it was retrieved a great quantity of fructan converted from sucrose.

而另一方面，利用重组基因技术来大量生产果聚糖转移酶，再进一步的利用酵素固定化这样的方法来回收酵素并得到大量的果聚糖产物。

To construct a retroviral vector carrying immortalizing gene hTERT, screen gene puro, reporter gene EGFP and recombination site sequence LoxP for reversible cell immortalization. Linearized pBABE-puro was cut by restriction enzyme Nhe Ⅰ and ligated with loxP double strands to construct a vector pBABE-puro-lox.

目的 探讨以hTERT作为永生化基因、嘌呤霉素乙酰转移酶基因puro作为筛选基因、加强型绿色荧光蛋白EGFP作为报道基因、loxp作为重组位点构建可回复性永生化逆转录病毒载体，为建立可回复性永生化细胞株莫定基础。

Alzheimer′s disease animal models were made by damaging bilateral nucleus basalis of Meynert of elderly rats (20-22 month) with quinolinic acid(150 nmol in 2 μL for each NBM, given d 3 after administration of test drugs). Activity of choline acetyltransferase was measured by radiochemistry assay. One time training passive avoidance step-down and water-maze spatial localization task were used to observe effects of ginsenoside of stem and leaf in combination with choline on learning and memory of AD model rats.

应用喹啉酸（每侧150 nmol，2 μL，给受试药后d 3注射）损毁老年大鼠双侧Meynert基底核制备阿尔茨海默病动物模型，放射化学法测定胆碱乙酰基转移酶活性，一次性训练被动回避跳台实验和水迷宫空间分辨能力测试，观察人参茎叶皂甙和胆碱合用对AD模型大鼠学习记忆的影响。

And Homo Sapien fetal brain cDNA Library was used to screen PRL-3-interacting proteins. 3. Identification and bioinformatical analysis of CDH22, a candidate PRL-3-interacting protein Interaction between PRL-3 and CDH22, a candidate PRL-3- interacting protein was identified by GST-pull down assay, co-immunoprecipitation assay and co-localization analysis in vivo and in vitro.

PRL-3相互作用蛋白的鉴定及其功能的生物信息学分析运用基于蛋白质水平的谷胱甘肽-S-转移酶融合蛋白沉淀、免疫共沉淀和共定位分析等技术对酵母双杂交系统初步筛选的结果进行体内和体外的验证。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。