转移酶

To determine the relationship between the expression of glutathione S-transferase-π and the response to chemotherapy in ovarian cancer.

目的：了解卵巢癌中谷胱甘肽S-转移酶-π的表达与化疗的关系。

Objective:To explore the clinical significance of glutathione Stransferase pi and Pglycoprotein in ovarian epithelial carcinoma.

目的：探讨卵巢上皮性癌谷胱甘肽S转移酶π和P 糖蛋白表达的临床意义。

The different fishes which are employed in the present studies are wild-type salmon, cultured salmon of freshwater and seawater, sea perch and fat greenling. The complete CT gene sequences of salmons are obtained by PCR amplification. The partial CT sequences of sea perch and fat greenling are obtained by in vitro cloning PCR method. Alignment of obtained CT sequences with other fish CT shows that CT appears to be well conserved among the same family. And the relative in taxonomy is far away, the similarity of different fish CTs is low. On the contrary, the closer the relative in taxonomy is, the higher the similarity of different fish CTs is. The sCT is expressed in pGEX-4T-X by recombinant form . We also succeed in the research of sCT expression alone by expression PCR. In addition, sCT antiserum is obtained using GST-sCT as antigen, and the high titer is tested by double immunodiffusion. In the rat bioassay, administration of 50 μg recombinant protein evoked significant hypocalcemia at 1 h after the data are analyzed by t-test.

本文用PCR方法克隆了野生鲑鱼、养殖鲑鱼的降钙素基因，并应用体外克隆PCR的方法首次克隆出鲈鱼、六线鱼降钙素的部分基因序列，通过对克隆的降钙素序列的比较研究，结果显示同一科的鱼降钙素序列保守性较高，同时，根据降钙素的部分氨基酸序列进行了降钙素相似性的比较研究，结果显示在分类学上，分类地位较远的鱼，其降钙素相似性较低，分类地位越接近的鱼，其降钙素相似性越高；我们利用谷胱甘肽S-转移酶(Glutathions S-transferase，GST)融合表达载体pGEX-4T-X对克隆的鲑鱼降钙素基因进行了融合表达研究，应用表达PCR的方法对降钙素基因的独立表达进行了初步的研究探索，并将纯化的融合蛋白作为抗原，获得了高效价的兔抗鲑鱼降钙素免疫血清；生物活性研究表明，大肠杆菌表达的融合蛋白具有显著的降血钙作用。

Objective To explore the relationship between postmortem interval and degradation of Hypoxanthine guanine phosporibosyltransferase mRNA in the kidney of rats.

目的：探讨大鼠死后肾次黄嘌呤鸟嘌呤磷酸核糖转移酶mRNA降解情况与死亡时间的关系。

Results:Normal rats as the donator of serum, using methanolic extract of CWC, 10 times equivalent dose for adults every day, intragastric administration twice a day of 8-hour interval, keeping on 3 days, hemospasia after 2 hours of the last administration was the better preparative method of the serum containing drug.

建立CCl4诱导的原代培养大鼠肝细胞损伤模型，大鼠为含药血清供体，以含药血清对损伤肝细胞增殖及丙氨酸氨基转移酶泄漏的影响两个指标，结合含药血清中五味子乙素的含量测定，分别采用单因素考察、多因素的正交试验，优选含药血清的制备方法，并确定体系中血清添加量。

In this review, we focus on a subclass of epigenetic regulators, namely histone methyltransferases.

在这篇综述中，我们集中在表观调节器的一个子类，即组蛋白甲基转移酶。

Objective:To study the hypoxanthine phosphoribosyl transferase gene mutation of rat peripheral blood lymphocytes and spleen lymphocytes induced by intermal radiation and of human peripheral blood lymphocytes induced by Y -ray.

目的：本实验主要是用克隆法研究放射性核素内照射诱发Wistar大鼠外周血淋巴细胞和脾淋巴细胞次黄嘌呤鸟嘌呤磷酸核糖转移酶基因突变及γ射线外照射诱发健康成年人外周血淋巴细胞HPRT基因突变情况。

The keti acid is converted to isoleucine by the activity of amino transferases.

酮酸被氨基转移酶转变为异亮氨酸。

Methods In situ method of terminal deoxytransferade-mediated dUTP-biotin nick and labelling staining was used to identify programmed cell death in 20 KAs. These cases also were examined with immunohistochemical technique for the expression of two protooncogenes, bcl-2 and bax, recently recognized to be involved in apoptosis.

用末端脱氧核苷酰转移酶介导的d-UTP生物素缺口末端标记技术，原位检测20例角化棘皮瘤中凋亡细胞；应用免疫组织化学技术检测皮损部位bax和bcl-2基因产物的表达。

Kazakh cycling team Astana's Lance Armstrong of the United States is photographed during a training session on July 13, 2009 in Limoges on the first of the two rest days.

哈萨克斯坦自行车队阿斯塔纳转移酶的兰斯阿姆斯特朗的美国拍摄在训练会议2009年7月13号在利摩日的首两个休息日。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。