转化的

According to the three deferent site selection methods, this thesis takes three transformations. The positive transformation (the numerical value larger, the result better) and the negative transformation (the numerical value smaller, the result better) are all included. A Matlab program is developed to predigest the osculating value method.

根据三种不同的铁路垃圾卫生填埋场选址方法，分别运用了三种不同的转化方法，对选址指标的正向和逆向进行不同的转化，开发出一个MatLab程序使得密切值法的运用更为简捷。

Attempt to use the material naturally conscious through simplification, transformation, to explain the various power of inner nature with seesawing, growing and declining; capture the "water" of nature in various forms, to be deconstructed and reorganized into a creative interpretation of the elements.

试图将自然的物质状态透过有意识的简化、转化，诠释大自然内部各种力量的拉锯、消长；撷取自然界中「水」的各种形态，加以解构重组，诠释转化成为创作的元素。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

In the separation of saponification resultants, the sodium soap of lanolin acid was converted to the calcium soap by Ca-C, which avoided emulsification in extraction and made separation easy, the complexing of cholesterol was limited through controlling the Ca-C dosage, which made subsequence separation simple.

对皂化产物分离过程，将皂化产物中的钠皂转化为钙皂，避免了萃取分离过程中的乳化现象，使分离更易操作；在钙皂转化过程中，通过控制钙盐C的加入量控制胆甾醇的配合，简化了后续的分离工艺。

The basic method will be to realize co-development of crop cultivation, animal husbandry and aquaculture while actively developing transportation and marketing and the processing industry with raw materials derived from crop cultivation, animal husbandry and aquaculture so as to tightly link grain production with transfer, processing and circulation, add value by manifold ways, increase the comparative benets of grain production and the income of the farmers, and ensure the sustained, stable development of grain production.

中国政府将采取一系列政策措施，促进粮食贸工农一体化经营，逐步建立以粮食为基础的高效农业体系，基本途径是，实行种植业和养殖业的复合发展，积极发展以种养业为原料的加工业和运销业，使粮食的生产、转化、加工和流通等环节紧密联结，多层次转化增值，提高粮食生产比较效益和农民收入，保证粮食生产的持续稳定发展。

People are particularly stressed out right now, and often emotional stress turns to physical stress, which turns to worse posture, which turns to pain, which turns to more stress, says Wendy Young, a certified ergonomist in Houston and author of the e-book, getinsideyourcomfortzone.com.

Wendy Young是一位工作学学者，他说，&现在的人们压力太大了，心理压力经常转化为生理上的压力，这会导致人们采用更糟糕的工作姿势，进而引起疼痛，又转化为更大的压力。&上一篇：有多少人上班的时候做私事？

People are particularly stressed out right now, and often emotional stress turns to physical stress, which turns to worse posture, which turns to pain, which turns to more stress, says Wendy Young, a certified ergonomist in Houston and author of the e-book, getinsideyourcomfortzone.com.

Wendy Young是一位工作学学者，他说，&现在的人们压力太大了，心理压力经常转化为生理上的压力，这会导致人们采用更糟糕的工作姿势，进而引起疼痛，又转化为更大的压力。&上一篇：双语阅读：有多少人上班的时候做私事？

People are particularly stressed out right now, and often emotional stress turns to physical stress, which turns to worse posture, which turns to pain, which turns to more stress, says Wendy Young, a certified ergonomist in Houston and author of the e-book, getinsideyourcomfortzone.com.

Wendy Young是一位工作学学者，他说，&现在的人们压力太大了，心理压力经常转化为生理上的压力，这会导致人们采用更糟糕的工作姿势，进而引起疼痛，又转化为更大的压力。&80块钱就能买到的口译考试冲刺！

The maximum MTG activity in the culture broth was 5.04u/mL and increased by 21.8% compared with the control. With the addition of CTAB, pro-MTG was activated to become MTG. CTAB could enhance the production of pro-MTG by relieving the pr...

初步研究表明，CTAB的主要作用是促使谷氨酰胺转胺酶的酶原转化为成熟酶，因此，在发酵过程中添加适当浓度的CTAB，可使酶原快速、完全地转化为成熟的MTG，解除酶原的产物抑制作用，促进了细胞产酶。

Kundalini takes ***ual energy in its raw form and converts it into fine spiritual energy of an extremely high frequency, which then allows the fruition and activation of paranormal activities such as good quality OBE's, telepathy, matter/energy conversion, and communication with entities that inhabit the vast areas of our multi-dimensional universe.

昆达里尼获得自然形态的性能量而将它转化为纯粹的非常高频率的精神能量，于是精神能量允许超正常行为例如好品质OBE、心灵感应、物质/能量转化与居住在我们多维宇宙广阔地方的实体沟通的实现和激活。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。