转化的

PartⅡTransform the yeast, screen the excellent expression yeast strain, induce the yeast to express the fusion protein and depurate the target protein. The recombination plasmid was transformed into the yeast GS115 successfully by means of electrotransformation. The excellent yeast strain was got by G418 screening. We achived the yeast supernatant including target protein induced by methanol.

第二部分：酵母的转化、蛋白高表达菌株的筛选、重组蛋白的诱导表达及纯化实验利用电转化的方法，成功地将重组质粒pPIC9k-PTD-hA20导入毕赤酵母GS115，并通过G418浓度梯度筛选得到了蛋白高表达菌株，并对蛋白高表达菌株进行甲醇诱导表达，得到了含有目的蛋白的酵母上清液。

This study explored a preliminary image with the mapping relationship between music and mapping laws, a transformation of images to the music algorithm, the image transformation to the music for a bold attempt to make people feel the use of auditory visual enjoyment effect.

详细说明：本研究初步探索出了图像与音乐之间的映射关系和映射规律，提出了一种图像向音乐转化的算法，对图像向音乐的转化进行了大胆的尝试，使人们用听觉感受到了视觉享受的效果。

Most of scholars only emphasize on Pierre Boundier's statement about capital belligerence but ignore his equal concern on capital reciprocal transformation.

多数学者往往强调布迪厄关于资本斗争性的阐述，而忽略了布氏对资本相互转化特性的同等强调，本文则主要从资本转化的角度去描述景区经营权转让背景下的政商关系是如何发生"从'商业化'到'去商业化'"的改变。

Using logistic and linear regression, we modeled the probability of transition from EM in 2005 to TM in 2006 in relation to medication use status at baseline.

应用logistic回归和线性回归分析，我们模拟了从2005年的EM到2006年的TM转化的概率，这种转化与基线的药物使用状况有关。

At present, regeneration system of cotyledons, hypocotyls, and stems, protoplasts and cotyledons codes have been established among Cucurbitaceae. There are several methods of transformation, such as agrobacteria -medidated genetic, particle gun, pollen tube passage, DNA soaking, ovary embryonary sac injection. Transgenic is tending to antivirotic and delayed maturity. Selection marker used often is NPT II.

目前，在葫芦科植物中已建立了子叶、茎尖、胚轴、茎段、原生质体和子叶节等的再生体系；用于遗传转化的方法有农杆菌介导法、基因枪法、花粉管通道法、DNA浸泡法等；用于转化的目的基因多为抗病毒病基因和延迟成熟的基因；NPTII基因为最常用的筛选标记基因。

DNA insertional mutagenesis is one of the most effective ways to identify pathogenicity genes of phytopathogenic fungi.

DNA插入突变是标记、分离功能基因的有效途径之一，ATMT是丝状真菌遗传转化的一个的新的技术，具有转化效率高、成本低，操作方便和重复性好多重优点。

Agrobacterium tumefaciens-mediated transformation is a new technique for insertional mutagenesis of filamentous fungi, and it has many advantages such as high efficiency、 low cost、 easy operation and good repetition.

DNA插入突变是标记、分离功能基因的有效途径之一，ATMT是丝状真菌遗传转化的一个的新的技术，具有转化效率高、成本低，操作方便和重复性好多重优点。

The hygromycin resistance gene is a marker gene to help sort out the transgenic mushroom cells from the non-transgenic cells, Dr. Romaine explained. What we are doing is taking a gene, as for example a drug gene, that is not part of the mushroom, and camouflaging it with regulatory elements from a mushroom gene.

潮霉素基因是一个标记物，它能筛选转化了和未转化的蘑菇细胞，Romaine教授解释说，我们做的就是得到这样一个基因，例如它是一个药物基因，它不是蘑菇基因的本身的组成部分，它伪装成蘑菇基因要做一个调整。

Methods genomic dna was extracted from different stages of malignant transformation and tumorigenicity in inoculated athymic mouse 16hbe cells induced by cadmium chloride and methylating status of genome p16 gene promoter was observed with methylation-specific pcr and then compared with the controls.

从各组cdcl2恶性转化不同阶段及接种裸鼠成瘤的16hbe细胞中提取全基因组dna，采取甲基化特异性pcr法(methylation-specific pcr，msp)检测该基因组p16基因启动子区的甲基化状况，与非转化的16hbe对照细胞进行比较，并用去甲基化因子5-azac (5-aza-2'deoxycytidine)处理有异常甲基化的细胞。

Consequently,in this study we introduced genes that encode the xylose isomerase and xylulokinase precisely under the control of a strong,constitutive glyceraldehydes-3-phosphate promoter by PCR-mediated overlap extension,which is in charge of xylose assimilation;and also we introduced genes that encode the transaldolase and transketolase precisely under the control of enolase promoter,which is in charge of xylose utilization,then transformed plasmid with the two operons into Z.

因此，本文通过代谢工程手段，将负责木糖代谢转化的木糖异构酶基因和木酮糖激酶基因置于强组成型启动子3-P-甘油醛下；同时将负责木糖转化利用的转醛醇酶和转酮醇酶基因置于强组成型启动子烯醇酶下，通过电转的方式将上述四个基因转入到Zymomonasmobilis CP4中，从而在运动发酵单胞菌中构建起一套完整的木糖代谢途径。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐