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MethodWiththe competent germinating epicotyl of cotton as receptor in exogenous gene transformation,NPTIIgene carrying selection marker and GFPreporter gene were mediated into cotton by agrobacterium-mediated transformation.The pre-culture time,bacterial-i mmersing ti me,co-culture ti me and antibiotics concn.,etc of using agrobacterium-mediated transformationinthe transformation of competent germinating epicotyl of cotton were studied.

方法以棉花感受态萌动的上胚轴作为外源基因转化的受体,用农杆菌介导法将携带有筛选标记NPTII基因及GFP报告基因导入棉花,研究了农杆菌介导法用于棉花感受态萌动上胚轴转化的预培养时间,浸菌时间,共培养时间及抗生素处理浓度等。

The establishment of tall fescue regeneration and genetic transformation systems was summarized.

本文对高羊茅植株再生体系的建立及遗传转化方面的研究进展进行了综述,介绍了国内外高羊茅遗传转化的历史和现状。

The results of the electrolysis showed that phosphite was transferred,to a certain degree,to hypophosphite with lower transferring rate and couldn't reach the requirements of industrial production,thus the cause of l.

本文通过电化学合成方法将化学镀镍过程中产生的亚磷酸盐转化为次磷酸盐的研究,首先根据理论分析和循环伏安曲线扫描分析,预测上述转化的可能性。

Inducible and constitutive expression vectors for monocotyledonous plants were constructed and used to transfer into maize calli. Plants were differentiated and regenerated from the positive calli screened with gradient concentration of hygromycin. 4 transformed plants with inducible expression vector and 2 with con

PCR检测获得4株用诱导型表达载体转化的植株和2株用组成型表达载体转化的植株,为进一步研究抗逆相关转录因子基因分eb在单子叶中的表达调控,培育抗逆玉米材料打下基础。

On the basis of experiment results,and theory of soil water dynamics and solutetransport,mathematical models of NTT are established for the field conditions.

在试验的基础上,根据溶质运移理论及土壤水动力学原理,建立了田间条件下氮素运移与转化的数学模型,对田间淹水稻田和旱作农田中氮的运移、转化过程进行了数值模拟研究。

we use the unity and orthogonal of Hubbard operators to give the transforming formula of Pauli matrices and Hubbard operators, by using these transforming formula we realize the change from Pauli matrices to Hubbard operators.

方法我们利用Hubbard算符的归一性和正交性给出了Hubbard算符和Pauli矩阵之间转化的运算公式,从而实现了哈密顿量表示方式的转化。

Ultra-micro structure of dolomite is investigated, we put forward that interface chemistry and geochemistry in nanometer hole should affect on the dolomization.

发现苏皖凹凸棒石粘土中白云石属于纳米颗粒,研究了白云石超微观结构特征,提出纳米孔界面化学和地球化学对白云石化的可能影响,初步模拟实验验证白云石化与纳米孔界面化学、纳米孔地球化学的关联,白云石交代过程中成分转化和结构转化的多步骤过程。

A reproducible and efficient transformation system for indica rice was developed based on microprojectile bombardment of embryogenic callus.

以籼稻胚性伤组织作为基因枪法转化的靶材料,建立了可重复的、高效的籼稻转化系统。

First, the in vitro metabolism of 6-chloro butyl phthalide was investigated and a HPLC method for the study of the in vitro metabolism of butyl phthalide analogues was developed.

首先研究了6-氯正丁苯酞的体外代谢,建立了研究氯丁苯酞及其类似物体外代谢转化的HPLC分析方法,并弄清了它们代谢转化的一般规律。

In order to investigate the electrotransformation condition of Sinorhizobium meliloti variable curve was devised by different cell cycles time,resuscitative buffer,electrotransfection buffer,pulsed electric field and resistance.

为了研究苜蓿中华根瘤菌电转化的条件,通过对不同的细胞生长周期、复苏缓冲液、电转缓冲液、电场强度和电阻对电转率影响程度来作出变化曲线,以确定电转化的最优条件,从而提高苜蓿中华根瘤菌电转化率。

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It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发,分析对称连杆曲线上鲍尔点的产生条件,提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品,年生产总值3000万美元,产品价廉物美、选料上乘、质量保证,深受国内外客户的青睐

The eˉtiology of hemospermia is complicate,but almost of hemospermia are benign.

血精的原因很,以良性病变为主。