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Mature embryos of wheat from cultivar EM12 in Hubei province and mode variety Bobwhite were used as target materials.

以湖北省小麦品种'鄂麦12'和模式品种'Bobwhite'为材料,成熟胚为转化受体,优化基因枪转化法的轰击压力、轰击距离、选择剂等因素,建立以小麦成熟胚为转化受体的高效转化系统。

The phenotype analysis displayed that number of transformants variety is 22 in growth rate, 30 in colony color, 32 in conidium-produced ability and 18 in conidium morphology.

通过表型分析,筛选出22个生长速度变异的转化子,30个菌落颜色变化的转化子,32个产孢能力减弱的转化子和18个孢子形态变异的转化子。

The hydantoin convert enzyme used in this essay is D2 hydantoinase, which has very high convert activity for D2 hydantoin. Therefore, if the used process of conversion do not occur the enzyme, the racemizatoin of hydantoin will be a speed limited step for the process that hydantoinase produce optical active amino acid.

本文采用的海因转化酶系中所含为 D2海因酶,对D2海因的转化活性非常高,由此可见,如若采用的转化体系中没有消旋酶的存在,则海因的消旋成为海因酶转化生产光学活性氨基酸的一个限速步骤。

The changes of the ion exchange percentage E of the interaction of both in the presence of the glycine have been presented.The results are that the effects of the transform sand and untransform sand on the adsorption are different,which agree with the reality and the theory inferrer.

结果表明:当沉积物分别为转化沙和未转化沙时,其对重金属的吸附作用的影响是不同的,且实验结果与理论推测和实际是一致的;甘氨酸的存在对不同金属离子分别与转化沙和未转化沙相互作用的离子交换率E-pH曲线的影响各不相同。

In this review,the progress on present range for plastid transforma-tion was described and the difficulties among the transgenic process were also analyzed from several critical factors such as tissue culture,vector...

本文总结了近年来质体转化在各种植物上的研究进展,并针对影响质体转化成功的因素,从组织培养、转化载体和标记基因等几个角度进行了分析。

A process for partial conversion and low-temp transform of CO in the synthetic ammonia plant where coal is used to generate gas features that the Fe-Mo catalyst for partial conversion of CO, anti-poisoning agent and Co-Mo catalyst for low-temp conversion are used and the temp of semi-water gas is controlled to 200-260 deg.C at entering anti-poisoning layer, and 150-250 deg.C at the segments of said Co-Mo catalyst layer, and lower than 300 deg.C at bed layer to prevent high-temp reversal of sulfurizing reaction and make the catalyst activity optimal. Its advantages are long service life of catalyst, low system resistance, saving energy and convenient operation.

本发明是中小合成氨厂以一种CO部分转化抗毒保护层配低温变换的工艺,涉及煤造气合成氨厂0.6~2.0MPa半水煤气变换工艺流程,该工艺用铁-钼CO部分转化催化剂、抗毒剂,配钴-钼低变催化剂,半水煤气进入部分转化抗毒层温度为200~260℃,进钴-钼低变催化剂各段温度为150~250℃,床层温度控制在300℃以内,避免高温下反硫化反应发生,保护催化剂活性处最佳状态,寿命延长、系统阻力小、节汽、节电,具有比其他变换工艺更好地节能效果,操作方便,有效生产时间长,经济效益显著等特点。

The results show that the addition of accelerators to the treating bath will accelerate the coating formation, and the added ammonium salt and chloride will change the Ce content in the coating from 5.93% to 4.15% and 9.27%, respectively. The added accelerators of ammonium salt and chloride could also increase the coating anti-corrosion performance to 2.6 times and 6.6 times against that treated without accelerator, respectively, and increases the micro-hardness of the Al surface from HV68.8 to HV389 and HV450, respectively.

结果表明:处理液中添加成膜促进剂可提高转化膜的生长速度,并且添加铵盐、氯化物后转化膜的铈含量从5.93%改变为4.15%和9.27%;还发现添加成膜促进剂铵盐、氯化物后转化膜耐腐蚀性提高为原来的2.6和5.6倍,其中后者的耐点滴腐蚀能力优于传统铬酸盐转化膜,并且还可将铝合金表面的显微硬度从HV68.8增大至HV389和HV450。

The optimal conditions for electroporation transformation of Pichia Pastoris were obtained with the maximum transformation efficiency of 36 transformants/μg plasmid DNA when the intermediate logarithmic phase yeast cells were used for competent cells, and the electroporations were under the conditions of voltage of 1.5kV and 0.15mg / mL plasmid DNA and 0.2 cm cuvettes.

结果表明:当采用对数生长中期的菌体制备感受态细胞、电压为 1.5kV、质粒浓度为 0.15μg/μL和 0.2 cm 电转化杯时,转化效率达到最大值,为每微克质粒 DNA 36个转化子。

But with the increase of the three-way catalytic converter's work time, the conversion efficiency descents constant

但是随着三元催化转化器使用时间的不断延长,其转化效率不断地下降,因而,对三元催化转化器转化效率的实时监控已成为人们关注和研究的问题。

As PTDT does in threshold traits, PTDT is valid not only for different QTL effect level, but also for maker with multiple alleles and multiple tightly linked markers.(2) Under an appropriate selection ratio s (in this study, s is 0.2, 0.4, 0.6, 0.8, respectively), the power of PTDT can be improved and the genotying individuals can be decreased using selective genotyping design. However, the power of PTDT is related with population size and population structure, an appropriate selection ratio can be defined by simulation based on the existing data.(3) Among the three transforming methods, mixed-family selection is the best, full-sib selection has same power to mixed-family selection in many parameter combinations, and Estimated Breeding Value selection is inferior to them.

数量性状QTL定位的模拟研究结果表明:(1)数量性状经有效转化后,PTDT对数量性状QTL定位保持了阈性状QTL定位的稳健与高效,对不同效应大小的QTL(10%,30%,50%)PTDT都是一个有效的分析方法;(2)在多等位基因标记、多标记方面,PTDT的检验功效与阐性状分析时一样高效;(3)在合适的选择率下(本研究的分别为0.2,0.4,0.6,0.8,1.0),选择性基因型测定不仅可以减少基因型测定的数量,而且可以提高PTDT的检验功效,但选择性基因型测定的PTDT检验功效很大程度上与群体大小和结构有关,这个可根据具体情况通过模拟找到一个合适的选择率;(4)三种转化方法的转化效力结果表明,家系内混合家系选择的转化效力最高,家系内全同胞选择次之,估计育种值选择的转化效力较差。

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