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The final concentration of target products(2,3-BD and acetoin) in a fed-batch fermentation carried out under the optimized conditions was 42.60 g/L with a productivity of 0.76 g/L·h in 56 h, corresponding to 23%of yield from glucose.

在该条件下进行批式流加发酵,可以获得目标产物浓度为42.60 g/L,生产强度为0.76g/,葡萄糖的质量转化率为23%。

Therefore,K. pneumoniae CICC 10011 is a potential strain for 2,3-BD production.Thirdly,a novel aqueous two-phase system for the recovery of 2,3-BD from fermentation broth was developed employing isopropanol/(NH_4)_2SO_4 system.The results showed that most of target products(2,3-BD and acetoin) can be partitioned efficiently into the top phase,while most of glucose can be partitioned into the bottom phase.

通过对批式流加发酵过程中两株克雷伯氏菌代谢流量的分析表明,K.pneumoniaeCICC 10011代谢产生的各种有机酸和乙醇浓度均明显低于K.pneumoniae DSM 2026,而目标产物浓度以及产物转化率却相对较高,是发酵2,3-丁二醇的优良菌株。

The optimum initial substrate concentration for cultivation of inoculum was 8%.The optimum conditions for batch fermentation were 0.04vvm of air flow,5.8 of pH value.The fed-batch fermentation showed that the productivity of Klebsiella pneumoniae CICC 10011 was better than K.pneumoniae CGMCC 1206.The maximum concentration of 2,3-butanediol and acetoin was 103.38 g/L (90.98 g/L of 2,3-butanediol),and the yield reached 82.2%of its theoretical value.The fermentation conditions for K.pneumoniae.

确定种子培养的最适初始底物浓度为8%;间歇发酵的条件为:微氧发酵,通气量为0.04vvm,pH值为5.8;通过批式流加发酵实验表明:Klebsiella pneumoniae CICC 10011的代谢产2,3-丁二醇能力要远高于K.pneumoniaeCGMCC 1206,2,3-丁二醇最高浓度达90.98g/L,2,3-丁二醇与乙偶姻浓度之和最高达103.38g/L,转化率为理论最大值的82.2%,其发酵工艺还有待于进一步优化。

Some methods of characterization and determination of transiormation ratio irorn acetylamine to amine in the deacetylation of chiun have been reviewed and evaluated.

本文对甲壳素脱乙酰基制取壳聚糖的反应中从乙酰胺基转变成胺基的转化率的各种表征方式和测定方法进行了详细综述和评价。

The result of the experiment shows , polysaccharide dosage groups have apparent promoter actions to the transition of normal rats' achroacyte.

实验结果显示:多糖各剂量组对正常小鼠的淋巴细胞转化有明显的促进作用其中VLP一1在 IOOmg/蛇·d剂量下作用最好,淋巴细胞转化率高达69.5013%。

The results showed that the conversion of adipic acid in the batch step and the Continuous step were more than 68% and 98%, respectively, at the catalyst level of 5%.

结果表明,当DNW-I型催化剂用量为5%(以己二酸计,m/m,下同)时,己二酸间歇及连续醋化后转化率分别大于68%和98%。

The transesterification reaction were studied under organic solvents systemThe dosage of diatomite immobilized lipase of Enterobacter aerogenes is 1000 U, ethanol as acyl receptors(alcohol:oil molar ratio is 4:1,2 times add),5 mL n-hexane, 180 r/min,35℃,48 h,and the conversion rate of 92.91%.

有机溶剂体下催化生产生物柴油硅藻土固定化产气肠杆菌脂肪酶用量为1000 U,乙醇为酰基受体(醇:油摩尔比为4:1,2次等量加入),5 mL正己烷,180 r/min,35℃,反应48 h,转化率达92.91%。

In aluminothermic reduction and nitridation, the reaction speed increases with the temperature, the optimum temperature is 1460℃.

严格控制反应条件,产物的氮含量可达到22.0wt%(对于z=3的β'-Sialon,氮的理论含量为24.7wt%,此转化率在有关天然原料合成β'-Sialon粉体的报道中目前是最高值。

chelating fiber with amidoxime group, synthesis, structure, property

第二种合成路线得到的偕胺肟基纤维氰基转化率可到100%,但合成路线要经过引发剂引发、丙烯腈单体接枝两步之后,才能进行偕胺肟化。

Increasing the degree of conversion from nitrile group to amidoxime group on the surface of PAN fiber caused not only higher Fe3+ content of Fe-AO-PAN, but also its stronger catalytic performanceon dye degradation in aqueous solution.

PAN纤维腈基转化率的提高不仅能够明显增加Fe-AOPAN表面结构中Fe3+含量,而且可显著促进其对染料氧化降解反应的催化作用。

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