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Were done and no positive reaction was found.5. The DNA of the young leaves of 132 Bt transgenic plants was extracted to do PCR Five positive inversion plants were obtained and the inversion rate was 3.8%.6. The DNA of fire positive inversion plants obtained in Dl generation was extracted and tested. Two stable positive inversion plants were obtained in D2 generations.7 The way of flourescent mirror flat was used to observe the pollen tube extension circumstance in the flower post after self-pollination.

检测了132株,没有发现阳性反应。5、对转入Bt基因的132株植株的幼叶提DNA,作PCR检测。D1代获得5株阳性转化植株,分别是90035的4株,85—593的1株,转化率为3.8%。6、将D1代获得5株阳性转化植株的种子在温箱中发芽,提取DNA检测,得到D2代稳定遗传的阳性转化植株2株。7、应用荧光镜制片法观察大豆自花授粉后花粉管在花柱中延伸情况。

Longer reaction time (48 h) and increase of hydrogen fluoride and catalyst are good for fluorination.

反应后的滤渣可再生为氧化汞循环使用,回收3次,反应的转化率和选择率均大于85%。

Reaction conditions are studied, and the result shows that it is better to adopt mercuric oxide or mercuric fluoride as fluorination catalyst with reaction temperature -20~-10℃.

结果表明,采用氧化汞或氟化汞作氟化催化剂、氟化氢作溶剂较好,适宜的反应温度为-20~-10℃,较长的反应时间(48 h)和增加氟化氢和催化剂的量有利于氟化。2-氯-5-三氯甲基吡啶的转化率和选择率均可达到97%以上。

The multiple alignment and phylogenetic analysis demonstrated that each genewas a member of a multigene family. 3. The function of 3GT gene was confirmed by Agrobacterium-mediatedtransformation of arabidopsis thaliana. Arabidopsis thaliana was transformed by floral dip method with AgrobacteriumGV3101 carrying expression vector pG3GT. Four transformants were selected for theirgrowth ability on 1/2MS medium containing 50mg/L kanamycin.

马铃薯野生种3GT基因的功能验证为了验证马铃薯野生种3GT基因的功能,构建带CaMV 35S启动子的表达载体pG3GT,转化农杆菌GV3101,用茵液浸泡花序法对拟南芥进行遗传转化,在含50mg/LKan的1/2MS培养基上筛选,得到4个抗性幼苗,转化率为0.13%,对移栽成活的3株进行PCR检测为阳性,Southern blot分析2株表现为阳性并为单拷贝整合。

The industrial application showed that the key process parameters, such as the regenerator dense phase temperature and the temperature under the first tray of the fractionator bottom, etc.

工业实践表明,应用此控制明显地改善了对再生催化剂温度、分馏塔底第一层板下温度等关键工艺参数的控制,提高了装置的转化率与轻油收率。

Result; The height and day-night variation of temperature make differences to the growth of mycelia and fruit body.

结果:温度高度及昼夜温差均对菌丝生长及子实体生物转化率有显著影响。

Result ; The height and day-night variation of temprature make differences to the growth of mycelia and fruit body.

结果:温度高度及昼夜温差均对菌丝生长及子实体生物转化率有显著影响。

Is the theory as a guide, the selection of a heterogeneous catalytic function of inorganic salt, the efficient preparation of compound catalyst, can make the original conversion rate of increase heterogeneous 4 to 5 percentage points, reducing the pollution of the environment and can significantly reduce Fumarate production costs and have a good economic and environmental benefits.

是以相关理论为指导,遴选有异构催化功能的无机盐,制备复合型的高效催化剂,可以使原有的异构转化率提高4~5个百分点,减少对环境的污染,且能显著降低富马酸的生产成本,有很好的经济效益和环保效益。

The experiment results show that the functional transformation reaction of polymers can also be conducted via phase-transfer catalysis successfully.

当以十六烷基三甲基溴化铵为催化剂且以甲苯为有机相时,反应效果最佳,反应温度为50 ℃时,反应8 h氯甲基的转化率可达到87%。

Space time yield of methanol and conversion of gas increase with pressure.

随着压力的升高,反应的时空产率和气体的转化率都在增加。

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