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The optimal timing for transformation is obtained using binary vector pGreen0229 drop on pollinated pistils at different time after pollination.

以中双9号为转化对象,研究优化了影响该转基因方法的主要因素。1探索了最适合的转化时间。

Consequently,in this study we introduced genes that encode the xylose isomerase and xylulokinase precisely under the control of a strong,constitutive glyceraldehydes-3-phosphate promoter by PCR-mediated overlap extension,which is in charge of xylose assimilation;and also we introduced genes that encode the transaldolase and transketolase precisely under the control of enolase promoter,which is in charge of xylose utilization,then transformed plasmid with the two operons into Z.

因此,本文通过代谢工程手段,将负责木糖代谢转化的木糖异构酶基因和木酮糖激酶基因置于强组成型启动子3-P-甘油醛下;同时将负责木糖转化利用的转醛醇酶和转酮醇酶基因置于强组成型启动子烯醇酶下,通过电转的方式将上述四个基因转入到Zymomonasmobilis CP4中,从而在运动发酵单胞菌中构建起一套完整的木糖代谢途径。

Public products and private products are not defined strictly and can not be transferred so meteorological information products as quasi-public products can ...

由于公共产品与私人产品之间并不是严格界定而不能转化的,因此作为准公共产品的气象信息产品在技术水平、市场范围、需求弹性发生变化的条件下可以向私人产品转化

The effects of H2/CO2 molar ratio, discharge voltage, and total flow rate on the conversion of CO2 and the energy efficiency were investigated.

分别考察了H2/CO2摩尔比、激发电压、原料气总流量对CO2转化的影响,分析了CO2转化的能量效率。

Agrobacterium tumefacines-mediated transformation system is routinely used for genetic transformation in a wide range of plant species.

利用根癌农杆菌介导的遗传转化方法,将CryⅠA基因转入生物防治真菌毛壳菌中,转化率约为40~180个转化子·10-7个孢子。

The transformation efficiency of potato via Agrobaterium tumefaciens system was studied,and the result showed that stem cutting is better than leaf for its high callus induction efficiency ,strong ability to differentiation and regeneration and easy to obtain.

并对遗传转化体系进行了优化:采用加入AgNO3有效的防止了外植体在培养过程中的褐化问题;对于转化过程中预培养的时间、共培养时间、农杆菌浸染时间和浸染浓度进行了试验,茎段的预培养2天、叶盘预培养3天,共培养时间茎段为3天、叶盘以2天为宜。

The role of steam-driven turbine is a rotary motion by the prime mover , will be sent to the steam boiler heat into mechanical energy of rotation, drive generator, generator is to convert mechanical energy to electrical energy equipment.

汽轮机是由水蒸气驱动作用旋转运动的原动机,将锅炉送来的蒸汽热能转化成转动的机械能,驱动发电机发电,发电机是将机械能转化成电能的装置设备。

The results shows that when temperature is lower than 300 ℃,NOx conversion efficiency increases with the increase of temperature and decreases with the increase of space velocity.

结果表明:在真实柴油机运行条件下的SCR反应中,反应温度低于300℃时,NOx标称转化效率随温度的增加而增加,随空速的增加而减小;在300~400℃,温度和空速对标称转化效率几乎没有影响。

But in sodium carbonate solution, any form of hydrogarnet can be decomposed into sodium hydrate alumino-silicate.

通过对钠硅渣和水化石榴石反应行为的热力学分析,发现高饱和系数( x >1)的水化石榴石不易被苛性碱分解,低饱和系数的水化石榴石易被分解而转化为钠硅渣,且钠硅渣也可以转化为水化石榴石,而在Na2CO3溶液中,任何形式的水化石榴石均能被分解生成钠硅渣。

Factors affecting transformation of shoot apex mediated by Agrobacterium tumefacien were also optimized.

对农杆菌介导高粱茎尖转化的因子进行了优化,获得较高转化频率的条件为:茎尖预培养时间3D,在OD600值为0.5农杆菌菌液中感染10MIN,共培养3D。

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推荐网络例句

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气,收缩臀部肌肉;拱起身体,尽量抬起头来,右腿伸直朝向天花板(膝微屈,以避免肌肉紧张)。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化,但却比去年同期高8%。

However, to get a true quote, you will need to provide detailed personal and financial information.

然而,要让一个真正的引用,你需要提供详细的个人和财务信息。