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转化

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The antifreezing ability of the transgenic plantlets was much higher than that of nontransgenic plantlets.

3进行试管苗抗冻试验,-5℃时转化植株和未转化植株的生长状况表现出较大差异。

The antifreezing experiments on the transgenic plantlets proved that there was obvious difference of growth between the transgenic and nontransgenic plantlets at -5℃.

PCR检测表明,转化植株的条带和质粒中的目的条带一致,而未转化植株没有扩增出任何条带,说明外源基因已经整合到草莓基因组上。

Transgenic plants were obtained by basta screening and PCR amplification.

转化T1代植株经除草剂Basta筛选和PCR扩增获得转化植株。

A transformant line obtained by transfection of NTH3T3 cells with c-Ha-T24-ras gene was treated with 5mmol/L benzamide,an inhibitor of PARP enzyme for 4 weeks.Some changes in cellular properties of the transformant line were observed.

用 5mmol/L PARP抑制剂苯甲酰胺处理经c-Ha-T24-ras活化癌基因转染得到的转化细胞系,处理4周后发现转化细胞系的某些细胞生物学特性发生了改变,呈现出正常细胞的某些特性。

Among them, 3 strains isolated from yellow water could convert lactic acid into ethanol and most strains isolated from Daqu could convert lactic acid into flavoring compositions such as ethanol, isobutyric acid, caproic acid or isovaleric acid etc.

其中,从黄水和大曲中分离到多种降乳菌,黄水中的3株菌可将乳酸转化为乙醇,大曲中的多数降乳菌可转化乳酸为异丁酸、己酸、异戊酸、乙醇等风味成分。

MSCs capsulated in alginate microspheres were induced by TGF-β3 and IGF-I, serving as the experiment group. While those cultured without TGF-β3 and IGF-I were taken as controls.

实验组用转化生长因子β3和胰岛素样生长因子Ⅰ联合定向诱导藻酸钠微球包被的骨髓间充质干细胞3周,对照组培养条件中无转化生长因子β3和胰岛素样生长因子Ⅰ。

The GPA1 gene was obtained via PCR amplification and was cloned in the two-hybrid DNA binding domain vector pGBKT7 The combinant plasmid was designated as pGBKT7-Ga.β-galactosidase assay indicatedthat Got did not have the property of self-activation. After pGBKT7-Ga was transformed to yeast pJ69-4A, we transformed Arabidopsis vegetative tissue two-hybrid cDNA library plasmids to yeast pJ69-4A containing pGBKT7-Ga.

通过PCR扩增得到GPA1基因并将其克隆到双杂交DNA结合域载体pGBKT7中,得到的重组质粒命名为pGBKT7-Gα,β—半乳糖苷酶活性鉴定表明Gα不具有自激活特性,将其转化到酵母菌pJ69-4A中,再以此为受体菌转化拟南芥绿色营养组织cDNA文库质粒。

Based on the acquisition –processing-abstraction of data, the structures an quantities of land use/cover change, the change of degree, the land use/cover dynamic degree and the index of the land use/cover dynamic degree were researched and analyzed comprehensibly in two different periodes(1990-1999,1999-2002) and sum up the conclusion.

结构变化不明显。水域、居民地、耕地动态度比较大,变化较为剧烈;未利用地动态度最小,变化缓慢。1990-2002年间水域、居民地、耕地与其他土地利用/覆盖类型间相互转化面积差额较大,转入面积大于转出面积,即在相互频繁转换同时单边转入明显;林地、草地、未利用地与其他土地利用/覆盖类型间相互转化面积差额不大,处于一种动态均衡状态。

At present, regeneration system of cotyledons, hypocotyls, and stems, protoplasts and cotyledons codes have been established among Cucurbitaceae. There are several methods of transformation, such as agrobacteria -medidated genetic, particle gun, pollen tube passage, DNA soaking, ovary embryonary sac injection. Transgenic is tending to antivirotic and delayed maturity. Selection marker used often is NPT II.

目前,在葫芦科植物中已建立了子叶、茎尖、胚轴、茎段、原生质体和子叶节等的再生体系;用于遗传转化的方法有农杆菌介导法、基因枪法、花粉管通道法、DNA浸泡法等;用于转化的目的基因多为抗病毒病基因和延迟成熟的基因;NPTII基因为最常用的筛选标记基因。

After 72 h induced by Progesterone and Glucocorticoid as well as Insuline,biological activities of IL-2 were assayed with morphological docimasia method.

结果显示,转染细胞均可表达IL-2,且所表达的IL-2有促进T淋巴细胞转化和淋巴母细胞成熟的活性;转染pcDNA3 OVP—IL2的输卵管上皮细胞表达产物在1:256稀释水平下仍具有促进T淋巴细胞转化和淋巴母细胞成熟的活性。

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Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气,收缩臀部肌肉;拱起身体,尽量抬起头来,右腿伸直朝向天花板(膝微屈,以避免肌肉紧张)。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化,但却比去年同期高8%。

However, to get a true quote, you will need to provide detailed personal and financial information.

然而,要让一个真正的引用,你需要提供详细的个人和财务信息。