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They had been walking about the place with some of their new friends, were my.ssbbww.com returned to the inn to dress themselves for dining with the same family, when the sound of a carriage drew them to a window, they saw a gentleman lady in a curricle, driving up the street.

他们到了蓝白屯的，便跟着几个新朋友到各处去溜达了一转，刚刚回到旅馆去换衣服，以便到一家朋友那里去吃饭，忽然听到一阵马车声，他们便走到窗口，只见一男一女，坐着一辆双轮马车，从大街上往这边来。

Day 2 Early morning private jeep tour to the White Desert, in your way to their you will see the old White Desert, the new White Desert, Flower Stones, Salt Lack and Springs, in the evening you will have your tent in the White Desert, enjoying the most magic sunset you can imagine and discover the secrets of the desert at night, at night our Bedouin friends they will do for you food by fire and over night there in tent in the White Desert,Day 3 Next day in the morning, you will be driven to see the quartz Crystal Mountain, Black Desert, back to the hotel in Baharia Oasis, having lunch and relax for bit, 2,30 pm transfer to the bus station taking 3 pm bus to Cairo, 8pm transfer from the bus station to your hotel in Cairo.

第2天 清晨私人吉普车参观白沙漠，在你的方式来面对你会看到旧的白沙漠，沙漠的新白，花石，盐缺乏和温泉，晚上你有你自己的白帐篷沙漠，享受最神奇的夕阳你能想像和发现的秘密沙漠的夜晚，我们的贝都因人在晚上的朋友，他们会为你做的食品及以上火晚上在帐篷中有白沙漠，第3天第二天早晨，您将会看到驱动石英水晶山，黑沙漠，回到酒店Baharia绿洲，吃午饭，放宽一点，2，30下午转移到公共汽车站公共汽车到下午3时到开罗晚上八时转由汽车站到您的酒店在开罗举行。

Pass through Ziqiang Tunnel, and turn right onto Bei-an Road. Then, turn

经自强隧道出隧道右转安，第一个红灯右转，即可到本校。

Goods receipt for stock transport order: at goods receipt the transported quantity is posted in the receiving plant from stock in transit into unrestricted-use stock (stock in quality inspection or blocked stock).

有关库存转储订单的收货：收货时，在接收工厂中，转储数量从在运库存过帐到非限制库存中。

A process for preparing the recombinant chicken gamma-interferon (rCHIFN-gamma) with high activirus activity includes such steps as cloning ChIFN-gamma gene from eukaryotic plasmid to transfer carrier by dual enzyme servenings to obtain recombinant transfer plasmid pFASTBACI-ChIFN-gamma, taking DH10Bac competent cell, adding IngpFASTBAC1-ChIFN-gamma, transposition, diluting to culture object by SOD culture medium, coating on Luria plate, culturing, choosing white clony, purifying, naming positive plasmid as Bacmid-ChIFN-gamma, extracting its DNA, and transfecting Sf9 cells.

高抗病毒活性重组鸡γ-干扰素的制取方法及用途，涉及一种广谱高效抗病毒基因工程产品的制取方法。将ChIFN-γ基因从真核质粒中经双酶切后克隆到转移载体中，获得重组转移质粒pFASTBAC1-ChIFN-γ；取DH10Bac感受态细胞，加入1ng pFASTBAC1-ChIFN-γ，转座后，用SOC培养基稀释培养物，涂布Luria平板，培养后，挑取白色菌落，Luria平板进行纯化，阳性质粒命名为Bacmid-ChIFN-γ，提取阳性质粒DNA；取上述重组DNA质粒转染Sf9细胞，制得高抗病毒活性重组鸡γ-干扰素。

Plant often subject to many biotic and abiotic factors in plant growth and development phases, and it must adapt to the environment through regulation of genes. Transcription factors play important roles in plant development and its response to the environment, so we need to research the function of transcription factors deeply.

植物在生长发育过程中经常遭受到许多生物和非生物因素的胁迫，需要通过对基因的调控来适应，而转录因子在植物的生长发育和防卫反应的表达调控中有着非常重要的作用，因此需要对转录因子的功能进行深入的研究。

The dose-dependent experiment revealed that Id1 antagonised E47 and Ets1 mediated transcriptional activation in a dose-dependent manner. With pGL3-870 as a template, site-directed mutagenesis of E-box and / or ETS-binding site was introduced into p16 promoter. E47 could not activate the transcription of p16 promoter with E-boxes mutated, and Ets1 could not activate the transcription of p16 promoter with EBS mutated. The synergy effect between E47 and Ets1 would occur only if the E-boxes and EBS were present. These facts demonstrated that E47 and Ets1 proteins were not recruited to the promoter by binding to other cis-elements or through interaction with other protein factors, however, they bound to DNA directly through respective consensus sequences in p16 promoter.

以pGL3-870为模板，构建了E-box或/和EBS点突变的p16启动子报告基因载体，将野生型和突变型的报告载体分别与pI-E47、pI-Etsl以及pI-E47+pI-Ets1共转染，发现E47和Ets1所诱导的转录激活作用依赖于启动子DNA上存在的各自的特异结合序列，二者的协同作用也只有当两个结合位点都存在时才会发生，说明E47和Ets1不是与其它作用元件或其它蛋白质因子相作用而被招募到该启动子的，它们是通过启动子上各自的特异结合位点与DNA直接结合的。

Chinese character transcription of all speakers' spontaneous monologues, including paralinguistic and non-linguistic information. Please refer to Specification of Transcription

对所有发音人的口语独白进行了语音到文字的转写，包括口语中出现的副语言学和非语言学信息的转写。

The results of transient transfection showed that the recombinant plasmid could be lowly expressed in the murine macrophage cell line RAW264.7 and murine melanoma cell line B16, both of which can endogenously express Tim-3, but not in the African green monkey kidney cell line COS-7 in which no endogenous Tim-3 could be detected.

结果 pGL3-Tim-3P2经PCR、双酶切及DNA测序分析鉴定正确无误；pGL3-Tim-3P2转染RAW264.7和B16细胞（两种细胞均可表达内源性Tim-3）24 h和48h，双荧光素酶活性检测显示其启动子相对活性约为pGL3-Basic空载体的2倍；而pGL3-Tim-3P2转染不表达内源性Tim-3的COS-7细胞后检测不到荧光素酶活性。

Go 2.8 miles. Turn right at W EI Camino Real. Go 1.3 miles. Turn right at Mariposa Ave. Go 0.2 mile.

前行2.8英里，在西伊尔喀梅诺里尔右转后，继续行驶1.3英里，到马里波萨大街右转。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。