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Using Pseudomonas syringae and Pantoea ananas , the dominant ice nucleation active bacteria strains from Yunnan province, as receptors of genetic engineering recombination, and Escherichia coli S17/pZJ25∶∶Tn5 as donator. By transposon Tn5 induced mutants method, the Tn5 was inserted and integrated into the genome of wild type INA + bacteria. INA + mutant as INA - when the linear continuous of INA + gene destroyed.

以云南省冰核细菌的优势种类丁香假单胞菌Pseudomonassyringae和菠萝泛菌Pantoeaananas为重组基因工程菌的受体菌，采用Tn5转座子诱变技术，以大肠杆菌EscherichiacoliS17/pZJ2 5∶∶Tn5作为转座子诱变的供体菌，使Tn5插入并整合到冰核细菌基因组中，使冰核基因的线性连续性被破坏，失去冰核活性。

Results:In 35 mm cell culture plate,2 μg DNA with 4μL liposome resulted in the highest transfection efficiency and the morphology of CNE-2Z cell can not saw by using microscopy after 8 h transfection.

结果：在35 mm培养皿中，2 μ gDNA与4μ L脂质体转染CNE-2Z细胞8 h，转染效率最高，且在显微镜下观察不到细胞的形态变化。

It is postulated that IP induces the release of endogenous protective molecules that interact with cognate receptors to activate PLC through mediation of the chincough virus sensitive proteins. PLC hydrolyzes membrane phospholipid and produces diacylglyccerol,that,by working together with calcium hydronium,translocates PKC from cytoplasm to cell membrane and activates PKC.

推测缺血预处理细胞保护作用机制是：缺血预处理引起内源性保护物质释放，作用于细胞膜上的相应受体，通过百日咳病毒敏感性G蛋白的介导而激活磷脂酶，磷脂酶水解膜磷脂，产生二酰基甘油(diacylglyccerol，DAG)，DAG和Ca2+协同作用于PKC，使其从胞浆转位到细胞膜并且被活化，PKC在膜上使底物蛋白磷酸化，引发一系列相关信号转导通路的激活，调控保护性基因的表达，从而提高细胞对缺血再灌注损伤的耐受性。

PCR, RT-PCT and PCR-Southern blot analysis indicated that phyB gene was successfully integrated into citrange genome and was transcribed in citrange.

PCR、RT-PCR和PCR Southern杂交结果表明：phyB基因已整合到枳橙的基因组，并得到了转录；转phyB基因枳橙表现出植株矮化、分枝角度加大、叶片变小、比叶干重增加，其叶绿素含量比对照高29.1％。

METHODS: Tum5 gene was amplified from plasmid pSPORT1Sfi by PCR technique and subcloned into the expression plasmid of lentiviral vector, pGCFU, to generate the lentiviral expression vector, pGCFUTum5. The correct Tum5 gene was confirmed by endoenzyme digestion and sequencing. Recombinant lentiviruses were produced by 293T cells following the cotransfection of pGCFU Tum5 and packaging plasmids-pHelper1.0and pHelper2.0. The resulting recombinant lentiviruses (GCFUTum5) which carried Tum5 and EGFPgene were then used to infect human umbilical vein endothelial cells.

采用PCR技术从含有tumstatin基因的质粒克隆模板 pSPORT1Sfi钓取Tum5基因，并将基因克隆到慢病毒载体表达质粒pGCFU中，构建慢病毒载体表达质粒pGCFUTum5，通过酶切、测序验证Tum5基因后，将pGCFUTum5质粒和包装质粒pHelper1.0，pHelper2.0共同转染人胚胎肾上皮细胞系293T细胞，获得携带Tum5基因和EGFP基因的重组慢病毒GCFUTum5，并转染靶细胞人脐静脉血管内皮细胞。

It can transfer gold foil paper to based material to achieve different material and color.

3转移印花：将转印纸或金泊纸转印到母材上，能得到与母材不同材料和颜色的效果。

Koala in the project, when the order Servo do not read, And because they require specific purposes needs to be done Jump read, it is necessary used Search function, Quick Jump to the designated location to read data.

在koala这个项目中，当 Servo不在做顺序读取，而因为某些特定目的要求需要做跳转读取，这时就需要用到Search功能，快速跳转至指定位置读取数据。

Go back to the first intersection, make a left turn, then turn right on the first intersection, it's on the east side of the street.

往回走到第一个交叉路口，左转，然后在第一个交叉路口右转，你要去的地方在路东。

Mathematical analysis predicts that constant rate shedding of autocrine ligands into a collapsing lateral intercellular space leads to increased local ligand concentrations that are sufficient to account for the observed receptor signalling; direct experimental comparison of signalling stimulated by compressive stress versus exogenous soluble ligand supports this prediction.

数学分析预测自分泌配体以常数频率脱落入萎陷的侧面胞间隙可以引起足以解释观察到的信号转导的局部配体浓度增加；直接实验比较由压力和外源可溶性配体刺激产生的信号转导也支持这个预测。

Witness the variety of wild landscape and wild life in this exotic land Tour itinerary Day 1 7Am Pick up from your hotel in Cairo, transfer to the bus station, take the bus to Baharia Oasis, 360 kilometers south west of Cairo, 1after noon, transfer from the bus station in Baharia to the hotel there, having lunch and starting jeep tour to the white desert, in your way to their you will see the old white desert, the new white desert, flower stones, salt lack and springs, in the evening you will have your tent in the white desert, enjoying the most magic sunset you can imagine and discover the secrets of the desert at night, at night our Bedouin friends they will do for you food by fire and over night there in tent in the white desert, Day 2 Next day in the morning, you will be driven to see the quartz Crystal mountain, black desert, back to the hotel in Baharia Oasis, having lunch and relax for bit, 2,30 pm transfer to the bus station taking 3pm bus back to Cairo, 8pm transfer from the bus station to your hotel in Cairo.

证人在这异国土地上的野生景观和野生动物品种旅游行程第1天上午7时从酒店选择在开罗，转移到汽车站，乘坐公车到Baharia绿洲，西南360公里的开罗，1after中午，转移从Baharia车站到酒店那里吃午饭，开始吉普车游以白色沙漠您的方式，对他们您将看到老白沙漠，沙漠中的新白，花石，盐缺乏，泉水，晚上，你有你自己在白色沙漠帐篷，享受您最神奇的日落可以想像，并且在夜间沙漠的秘密晚上，我们的贝都因人朋友，他们会为你做火的食品和白色沙漠帐篷里过夜，在那里，第2天在第二天早上，你将被迫看到石英水晶山，黑沙漠，回到了Baharia绿洲酒店，吃午饭，放宽一点，2，30下午转移到汽车站到下午3点走回开罗晚上八时转由汽车站到您在开罗酒店。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。