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Transcriptional activity of ACL recombinants normalized by Renilla was significant difference with pGL3-Basic expect for construct -27 and -15 (P＜0.01). Construct -919 contains highest activity. 3 times reduction of transcriptional activity from-919 to -679bp indicated that negative regulation factors located probably in this region. The activity started on construct -73 suggested the basal promoter activity was located within the -73 to +77bp region; Transcriptional activity of IDHβrecombinants was not significantly different between the recombinant -58 and pGL3-Basic. The activity started on construct -82, decreasing with the length of the fragment up to -164 in despite of a bit of fluctuation, and kept increasing from construct -164 up to -279. Thus, the basal promoter activity was located within the -82 to +16bp region, whereas the upstream 197bp conferred maximal transcriptional activity.

采用5'端缺失策略，结合启动子预测结果，分别构建了8个ACL基因和19个IDH3β基因启动子区重组子，将其转染猪PK15细胞系，并利用荧光素酶双报告基因系统检测了它们的活性，结果表明：在所构建的猪ACL基因5侧翼序列的8个重组子中，除pGL-ACL27和pGL-ACL15外，其它重组子的荧光素酶活性与阴性对照均达到极显著水平(P＜0.01)，表明它们均具有启动子活性，pGL-ACL919活性最强，到pGL-ACL679活性下降了将近3倍，说明从-919bp到-679bp区域可能存在负的调控位点，从pGL-ACL621到pGL-ACL73活性有小幅度下降，到pGL-ACL27活性降到与阴性对照水平无显著差异，表明维持该启动子的基本活性区域位于-73bp到+77bp之间；IDH3β基因启动子活性开始于pGL-IB82，然后虽然有些波动，但是活性一直下降直到-164bp处，之后活性又开始升高，直到-279bp处活性达到最高，这些表明维持该启动子的基本活性区域位于-82bp到+16bp之间，从-82到-279之间的179bp区域具有最高的启动子活性。

Fluorescence staining of cell framework and nuclei were conducted, the result of which revealed that the transfected cells displayed disorder in cell skeleton, shrinkage and cleavage in nuclei. It was illuminated that chromosome DNA occurred cleavage and fragment since the result of TUNEL detection was positive. The transfected cells also displayed more typical apoptotic character, including exposure of phosphatidylserine in the plasma membrane by Annexin V staining detection, shrinkage nuclei, chromatin condensation, plasma membrane blebbing and chromatin breakage fragments by electron microscopy analyses.

细胞骨架和细胞核染色结果表明，AIF基因转染细胞呈现细胞骨架的紊乱、细胞核固缩、分裂等变化；TUNEL检测呈阳性，说明有染色体DNA出现断裂；Annexin V染色检测到转染细胞出现胞膜磷脂酰丝氨酸的暴露；电镜观察的结果显示，转染目的基因组细胞呈现细胞膜出泡、细胞核固缩、染色体凝集和断裂等典型的凋亡细胞特征。

In addition, there are entrenched uses of referential metonymies observed in our corpus data, which reveals how our recurrent interaction with outer environment gives rise to the saliency of particular concepts and how these concepts, universal or culture-specific alike, further lead to language routinization.

除此之外，本篇研究观察到固定化指涉转喻(entrenched referential metonymy)的现象，我们认为这种固定化的转喻起因於人类与外在环境或文化社群的不断互动，当某些概念在互动过程中一再地被突显，这些概念将会频繁地被使用作转喻的来源范畴，并且进一步固定成惯例化的语言形式。

A type of transposition in which the transposon is removed from its original location to be inserted into a new location.

一种转座类型，转座时转座子从它的原始位置离开并插入到一个新的位置中。

The gene expression of chimeric virus could be detected in 293T cells transfected by SHIV proviral DNA.

用此 SHIV 原病毒 DNA 转染 293T 细胞，细胞中能够检测到嵌合病毒基因的转录与翻译；在细胞培养液上清中亦可检测到装配出的病毒颗粒。

Consequently,in this study we introduced genes that encode the xylose isomerase and xylulokinase precisely under the control of a strong,constitutive glyceraldehydes-3-phosphate promoter by PCR-mediated overlap extension,which is in charge of xylose assimilation;and also we introduced genes that encode the transaldolase and transketolase precisely under the control of enolase promoter,which is in charge of xylose utilization,then transformed plasmid with the two operons into Z.

因此，本文通过代谢工程手段，将负责木糖代谢转化的木糖异构酶基因和木酮糖激酶基因置于强组成型启动子3-P-甘油醛下；同时将负责木糖转化利用的转醛醇酶和转酮醇酶基因置于强组成型启动子烯醇酶下，通过电转的方式将上述四个基因转入到Zymomonasmobilis CP4中，从而在运动发酵单胞菌中构建起一套完整的木糖代谢途径。

The first control strategy has the disadvantages such as a large amount of computations, inherent pull-out torque, difficulties in realizing the adaptive control and so forth. For the rotor flux-oriented control, changing of load or the rotor speed will result in increased or decreased torque winding currents, which will change the magnetic field distribution in the air gap. Assumption that magnetic field is approximately equal to that generated by torque windings will affect the levitation performances.

分析表明：气隙磁场定向控制中存在最大转矩限制、非线性机械特性，难以实现自适应控制等问题；对转子磁场定向控制而言，当负载或转速变化时，由于转矩控制绕组转矩电流变化将导致气隙磁场发生改变，如果仍以转子磁场近似替代气隙磁场作为悬浮依据，必然影响到悬浮控制性能。

The wind that touches the prayer wheel is blessed by the power of the prayer wheel and then has the power to purify the negative karma and obscurations of any being it touches.

碰触到转经轮的风受转经轮的力量加持，从而具有力量，能净除任何被风碰触到的有情的恶业及障碍。

After dinner pipe Mingqiang went away after the two units, with Yang Meng-Tsan Chiang twisted my look on in the county did not buy copies of eating KFC on the back, and coming home to see Yi Xi-two people rely on exhausted fell asleep on the sofa , Yang sat down on a quiet seat next to doing winter work, Meng-Tsan Chiang mp3 driving while listening to music, reading fashion magazines, turned up in the afternoon sun was very warm, bathed in the branches Chanchanmianmian the middle, there is a ray of light exposure to the plum star body, Rou Rou's gently.

吃饭后管明强送走两人之后去了单位，孟灿带杨晓月在县城里转了转看没有买的吃了份肯德基就回来了，到家看易夕两个人靠沙发上疲惫的睡着了，杨晓月安静的坐到旁边的座位上做着寒假作业，孟灿开着mp3听着音乐边翻开时尚杂志看了起来，午后的阳光非常温暖，缠缠绵绵沐浴在树枝中间，有一丝光线照射到李子星的身上，柔柔的轻轻的。

Just one last dance...oh baby..just one last dance we meet in the night in the spanish cafe I look in your eyes just don't know what to say It feels like I'm drowning in salty water Afew hours left'til the sun's gonna rise tomorrow will come an it's time to realize our love has finished forever how I wish to come with you how I wish we made it through Just one last dance before we say goodbye when we sway and turn round and round and round it's like the first time Just one more chance hold me tight and keep me warm cause the night is getting cold and Idon't know where I belong Just one last dance The wine and the lights and the Spanish guitar I'll never forget how romantic they are but I know, tomorrow I'll lose the one I love There's no wayto come wyth you it's the only way to do Just one last dance before we say goodbye when we sway and turn round and round and round it's like the first time Just one more chance hold me tight and keep me warm cause the night is getting cold and Idon't know where I belong Just one last dance

我们来到我们第一次见面的西班牙咖啡馆，看着你的眼睛，我真的不知道要说什么好……明天的太阳还有几个小时就要升起，只有那么短暂的几个小时了，到了明天，我知道，我们之间的爱就会永远、永远的结束……其实我多么希望能够跟你在一起；一辈子都在一起……只有最后的一只舞了，在我们分开之前的，最后的一只，我们像我们第一次见面那样，转啊，转啊……这是最后的一次我们彼此紧紧相依，感受对方的体温，因为夜越来越冷，寒冷到我甚至不知道自己到底属于哪里……最后的一只舞，那摇曳的红酒，那暗昧的灯光，还有那个西班牙吉他，我不会忘记它们有多么的浪漫。但是我知道，明天即将到来，我就会永失我爱……只能这样了，我没有办法跟你厮守……那就跳这最后的舞吧，最后的一只舞，只有这最后的一只舞

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。