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Results All cases became amenorrheic during GnRH-α therapy. The enlarged uteri all decreased to normal or near normal size. Menstruation returned in 80～90 days after cessation of treatment. Three cases conceived within four menstrual periods. One of them resulted in the birth of a healthy 3 150 g male at 38 weeks gestation by cesarean section. The second pregnancy resulting after adenomyomectomy was terminated by emergent cesarean section at 30 weeks gestation because of threatened rupture of uterus. The third is now normal at 28 weeks pregnancy .The fourth has had 2 menstrual periods and is still being followed up.

结果 GnRH-α用药期间4例均闭经，血雌二醇于用药的第3周降到绝经期水平；用药6次后增大的子宫缩小至正常或接近正常。1例子宫后壁局限性腺肌瘤未行手术切除者，用药后腺肌瘤缩小73.7%。3例于腹腔镜术后、停药转经的4个月内妊娠；另1例停药转经2个月，现在随访中。3例妊娠转归：1例足月剖宫产分娩；1例手术切除肌壁间腺肌瘤者，孕30周时因先兆子宫破裂急诊行剖宫产术；1例目前孕22周。

Their fecundity outcome were followed-up after cessation of GnRH-α treatment. Results All cases became amenorrheic during GnRH-α therapy. The enlarged uteri all decreased to normal or near normal size. Menstruation returned in 80～90 days after cessation of treatment. Three cases conceived within four menstrual periods. One of them resulted in the birth of a healthy 3 150 g male at 38 weeks gestation by cesarean section. The second pregnancy resulting after adenomyomectomy was terminated by emergent cesarean section at 30 weeks gestation because of threatened rupture of uterus.

结果 GnRH-α用药期间4例均闭经，血雌二醇于用药的第3周降到绝经期水平；用药6次后增大的子宫缩小至正常或接近正常。1例子宫后壁局限性腺肌瘤未行手术切除者，用药后腺肌瘤缩小73.7%。3例于腹腔镜术后、停药转经的4个月内妊娠；另1例停药转经2个月，现在随访中。3例妊娠转归：1例足月剖宫产分娩；1例手术切除肌壁间腺肌瘤者，孕30周时因先兆子宫破裂急诊行剖宫产术；1例目前孕22周。

Ansochromogenes was transformed by pUC1169 from itself. 8 mutants lost ability to produce nikkomycin were screened from 4068 colonies of Vio〓 Thio〓 with Tn4560 transposition. Southern blotting suggested that Tn4560 was inserted in at least 4 positions of chromosomal DNA in S.

于39℃，MM／Vio条件下培养携带有pUC1169的7100孢子，Tn4560发生转座；筛选到4068个转座菌落，并从中得到8株尼可霉素阻断突变株；对这8株突变株的总DNA进行Southern杂交分析表明，Tn4560至少在4个不同的位点插入到7100的染色体上。

In recent years, it seems that the superficial Chinese transliteration "苗"(pronounced as "miao" in modern Mandarin Chinese, but as "mao" or "hmao" in medieval Chinese) and the English transliteration "Hmong" have been misleadingly essentialized by some anthropologists, and the differences associated with these externally referred ethnonyms were magnified to such an extent as if there had been two entirely different or unrelated ethnicities – one with the autonym "Hmong" and the other with "Miao"– in the world.

近年来，&苗&（现代汉语读音为'miao'，但汉语古音读'mao'或带前鼻音的'hmao'）这个表面上的汉文转写形式，与&Hmong&这个英文转写形式一道，被一些西方人类学家误导性地当成了实质；与这两种表面转写形式相关联的一些差异被放大到了如此无以复加的地步，以至于让人们也都误觉得，这世界上并存着两个不甚相干的民族，其中一个的族称是&Miao&，而另一个的族称是&Hmong&。

Expression of tert and hprt genes were detected simultaneous in all single-copy embryos developed at 4-C (65h-post hCG, 4/4), 8-C (75 h-post hCG, 4/4), morulae (93h-post hCG, 4/4) and blastocyst (118h-post hCG, 4/4) stages and the level of transcription increased.

结果表明，持家基因hprt在成熟卵母细胞受精前后，以及胚胎早期发育过程中均存在转录本。40%卵母细胞中存在的tert mRNA在受精后很快降解，检测不到；胚胎基因组在2-C早期开始转录tert mRNA，转录水平逐渐上升。

When he was out roaming and foraging and you would call out his name, he would come running at top speed, honking the whole way until he got close to you where he would dodge you, zig zagging around with a few victory roles turning in circles before settling down and calmly walking up to you with his tail wagging as if to say "hah, got-cha."

他在外面溜达觅食的时候，只要你喊他的名字，他就会以最快的速度朝你奔来，&鼾鼾&地一路叫着，跑到离你不远的地方他又会跟你玩起迷藏来，左转右转地走着，绕着圈，一副凯旋而归的模样，然后才静下来慢慢走到你跟前，摇摇尾巴，好像眨着眼跟你说&哈，总算找到你啦！&

Please a process ■ The purchase please a style * Purchase payment practice week knot make, each Tuesday is transfer pay day, special circumstances next Thursday consider feeling arrangement transfer payment;* All purchase list is confirm signing by the general manager assistant →finance department the cashier examine →finance the manager examine →general manager approval →cashier payment and notice supplier →the accountancy recheck and statistics;* Cash on delivery from purchase specialist confirm incoming goods amount, on the receipt signature of the supplier confirm payment amount of money, and at purchase list top signature confirm method of payment is cash payment, confirm without any error empress notice cashier payment, the accountancy recheck.

请款流程■采购请款*采购货款实行周结制，每周二为转帐付款日，特殊情况下周四酌情安排转帐付款；*所有采购单由总经理助理确认签字→财务部出纳审核→财务经理审核→总经理批准→出纳付款并通知供应商→会计复核并统计；*货到付款时由采购专员确认来货数量，在供应商的收据上签名确认付款金额，并在采购单上签名确认付款方式为现金支付，确认无误后通知出纳付款，会计复核（区分现金支付与转帐支付，确保不重复付款）。

The present invention has stepped motor and sync belt driver to drive the label roll, guide wheel and magnetic steel plate to separate the upper paper layer and the lower paper layer from the label belt, electromagnet driven cutter to cut the adhesive film between two successive labels, electromagnet driven magnetic steel block to transfer the label onto the label inserting board, and elevating and rotating manipulator to insert and stick the label inserting board into book seam.

本发明通过步进电机、同步带传动驱动卷标上下层纸带收卷轮的转动，上层纸带与标签及下层纸带与标签之间的分离通过一导轮和磁钢板实现，连续型卷标相邻两个标签之间的不干胶膜由电磁铁驱动的切刀切断，金属标签通过电磁铁驱动磁钢块撞击取签插板而使标签脱离下层纸带被转贴到取签插板上，取签插板手动插入或取出，或者用升降旋转机械手将末端贴有标签的取签插板插入翻开的图书中间夹缝内，使标签转贴到图书内纸上。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

In the standard measurement, such as bar code through a splitter, will be divided into two beams of light, a bunch of switch hitting the sensor photodiode array, and the other a bunch of change reach all parts of the observation mirror.

在水准测量中，条码的像通过一个分光器，将光线分成两束，一束转射到传感光电二极管阵列上，另一束转射到观测镜上。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。