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By retrovirus vector the foreign mdr1 gene could be efficiently transferred into bone marrow mononuclear cells of mouse in vitro, and it was confirmed that mdr1 gene was expressing stably and effectively in cells; in short terms there is no significant influence on the bone marrow MNC by transfection of mdr1 gene,but in the long run(in our study we had surveyed 2 months),there is a decline in the expression of apoptosis factor Bax in the MNC which transfected by mdr1 gene,in favour of the recovery of the receptor mouse;on the other hand it also poses a blance problem between multiplication and apoptosis in the process of receptor hematopoiesis after transplanting HSC which was transfected with mdr1 gene.

通过逆转录病毒载体可在体外将外源性mdr1基因转入小鼠骨髓单个核细胞中，转染的mdr1基因能整合到骨髓单个核细胞基因组中并有功能性表达；mdr1基因转入小鼠骨髓单个核细胞在移植后短期内对受体造血细胞没有显著影响，但从长期看来(本研究观察2个月)转染了mdr1基因的造血干细胞的凋亡因子Bax表达降低，更有利于受体鼠造血的恢复；另一个方面它也同时提出一个问题，即mdr1基因转染造血干细胞在回输受体后造血过程的增殖凋亡平衡。

Induced activation of Rac1,Cdc42 signal transduction pathway, monitoring the transfer of living cells activated sheet pseudopodium emerged and filopodia obvious morphological changes,such as measured values or fluorescence imaging of fluorescence intensity over time to continuously reduce until almost see the determination or observation.

诱导激活的Rac1、Cdc42信号转导通路，监测到转染激活的活细胞中出现片状伪足和丝状伪足等明显的形态变化，测定荧光值或者成像的荧光强度随着时间推移不断降低，直至几乎测定或观察不到。

B:Turn left outside here,go along until you come to the ninth road,turn right,go straight ahead,straight on at the round-about,down the hill,and turn right at the bottom.

从这里出去向左拐，向前一直走到第9大街，向右转，一直向前，走到环形路口，走下小山，在山脚下再向右转。

Turn left outside here; go along until you come to the ninth road; turn right; go straight ahead, straight on at the round-about, down the hill, and turn right at the bottom.

从这里出去向左拐，向前一直走到第9大街，向右转，一直向前，走到环形路口，走下小山，在山脚下再右转。

The car started, went down the lane, turned right, turned left, turned right again and came out on a secondary road.

车开动了，沿着小巷子驶下去，-会儿向右转，一会儿向左转，又再向右转，最后到了一条车辆比较少的公路上。

Now that the warmer months are coming, why don't you take the opportunity to travel around Siberia

既然更温暖的月份快要到了，你为什么不借此机会到西伯利亚去转一转呢？

Expression of M, NP, F and HN genes were detected and confirmed by the indirect immunofluorescence analysis. The syncytium formation was observed in the HeLa cells co-transfected with pCAGG-M, pCAGG-NP, pCAGG-F and pCAGG-HN recombinant plasmids at 48h post transfection.

纯化后的重组质粒通过脂质体单独转染HeLa细胞，经间接免疫荧光试验检测到了M、NP、F和HN蛋白的表达。pCAGG-M、 pCAGG-NP、pCAGG-F和pCAGG-HN重组质粒组合共转染HeLa细胞48h后，可以观察到明显的细胞融合现象。

A drilling apparatus (10) for earth boring operations is of the type having cutter blades (17, 18) mounted on a support body (12, 72, 82) at the lower end of a drill string and includes a novel arrangement of fluid bore sections (15, 23, 23', 63, 64, 65', 92) for delivering fluid under pressure into fluid discharge passages (50, 52) in each of the blades and a bifurcated pivotal mounting portion (16, 74, 91) on the body which enables the bore sections to supply fluid to the cutter blades through a ball joint (22) on the pivotal axis.

一种用于地面钻孔操作的钻井设备(10)，在钻柱的下端具有安装在支撑体(12，72，82)上的切割刀片(17，18)，并包括流体孔部分(15，23，23′，63，64，65，65′，92)和该支撑体上的分叉的枢转安装部分(16，74，91)的新颖结构，该流体孔部分用于在压力作用下将流体供给到每个刀片的流体排放通道中，该安装部分使的该孔部分通过枢转轴线上的球接头(22)能够将流体供给供给到该切割刀片。

The power band is remarkably linear, with no lapses or surges as the tach needle climbs to 6500, where a rev-limiter abruptly interrupts forward progress; so you upshift and all is well again.

扭力分布是显著线性的，当转数指针一路攀爬到6500转期间，并没有感觉丢失或巨涌。当排档转数达到极限，突然地打断你向前的步伐；你只需要换一个高档，一切将会再次很好。

METHODS: MyoD cDNA fragments were extracted from plasmids pEMSV-MyoD with polymerase chain reaction, and PCR was used to clone the whole-length gene of MyoD. After adding CACC sequence at 5' end, MyoD gene was cloned by orient topology into transfer ventor, pENTR/D-TOPO. Objective gene was transferred into adenoviral expression vector DNA via pENTR/D-TOPO vector. The recombinant adenoviral vectors transfected into HEK293A cells by using lipofectamine were packaged and amplified.

从pEMSV-MyoD质粒上用聚合酶链反应法扩增出MyoD cDNA片段，再通过聚合酶链反应使MyoD基因加上CACC序列接头，经过定向拓扑克隆使目的基因连接到转移载体上，再通过LR酶促反应，将目的基因转移到腺病毒表达载体DNA上，获得MyoD基因重组的腺病毒DNA，用脂质体转染法转染HEK293A细胞，包装扩增出MyoD基因重组的腺病毒。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。