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Marketing and Sales Organization 3.1 Sales Organisation Organization chart and description of sales staff; by product line, by division, by geographic distribution Describe the number of in-house sales personnel and their average years' experience with the Company and industry generally Describe the incentive arrangements with the sales professionals Describe any training provided to the Company's sales force Describe each sales person who will be transferred to newco (age, average experience with Company and indsutry, incentive agreements, training, performance) 3.2 Sales channels, representatives Description of the sales channel, legal relationship with sales offices, wholesalers and direct retailers and break down of sales by each (location, direct, reseller, distributor, etc…) List of key representatives, wholesalers, resellers, etc.

3月1日销售组织架构图销售组织与销售人员的描述;按产品线由师按地理分布描述一些在内部销售人员,其平均年经验的公司,业内人士普遍形容激励安排与销售专业培训说明该公司的销售队伍描述每个销售人员将转往新(年龄公司与制造业平均经验,激励协议、培训业绩)3.2销售渠道描述代表的销售渠道,同法律售楼处,直接打破、批发商和零售商销售的每(地点、直接、转售商、经销商、等等::)主要代表名单、批发商、转售等为每个大字补偿安排,服务市场,成交额达到长短关系满意度形容任何重大历史趋势和预测方法采用的销售及分销公司和服务行业普遍三月三日跷跷描述营销活动三月四日六月三日活动说明客户名单、描述(名称、地点、工业类、每年营业额、主要产品卖给顾客)前50位顾客提供一份合同五十强顾客。

On the other hand, if a 32-bit program calls a 16-bit DLL, then the thunk is in the opposite direction: from 32 bit to 16 bit.

另外,如果一个32位程序呼叫一个16位DLL,那么thunk就存在于相反的方向:从32位转变成16位。

A thunk typically occurs when a 16-bit application is running in a 32-bit address space, and its 16-bit segmented address must be converted into a full 32-bit flat address.

当16位应用程序在32位地址空间进行运行的时候,应用程序的16位段地址必须转化成32位平坦地址,这时候thunk是很容易见到的。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank,选择包含人主要T细胞抗原表位序列的人PSCA基因片段,应用异种化抗原设计技术,保留人T细胞抗原表位,设计异种化PSCA融合抗原片段;(2)根据核酸序列按中心模板法设计引物,应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因,以PCR、限制性酶切和DNA序列测定法进行鉴定:(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点,采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4),并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中,转染293T细胞,借助免疫荧光+流式细胞术考察插入片段表达效率,最终选定PSCA_3片段进行下一步研究;(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下,插入pVAX1-neo-IRES—GM/B7载体中,构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB,并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况;(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞,制备小鼠前列腺癌模型,并采用股四头肌肌肉注射+电脉冲法(Electroporation,EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB,同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照,通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率,来评价该DNA疫苗在小鼠体内的抑瘤效果。

Methods The 201 cases in 1979~1996 were retrospectively summarized. The clinical features including chief complaints, sex distribution, age at first visit, laterality, type of presentation, ocular deviation in the primary position, refractive errors, amblyopia, globe retraction, change of the palpebral fissure, upshoot and downshoot in adduction, binocular single vision, and its associated ocular and non-ocular anomalies were analyzed.

自1979~1996年回顾性地总结了201例DRS,对它的临床特征,包括主诉、性别分布、初诊年龄、侧向状态、表现类型、原在位的眼位偏斜、屈光不正、弱视、眼球后退、睑裂改变、内转时眼球急速上转和下转、双眼单视及其伴随的眼部和非眼部异常等予以分析。

This article studied differences in postpartum fatigue and birth outcomes between women who pushed immediately and those who delayed pushing during the second stage of labor. Data were collected from primiparous women in their 38th to 42nd gestational week who did not receive epidural analgesia during labor and were free of complications during pregnancy. Using a quasi-experimental design, 72 participants selected by convenient sampling were assigned based on individual participant's preference to either an experimental or control group. For the experimental group, pushing was delayed until the point after full cervical dilation at which the mother felt a strong physical pushing reflex, the fetal head had both descended to at least the +1 level in the pelvis and turned to the occiput anterior position, and uterine contractions were at least 30 mmHg. For the control group, the physician instructed mothers to begin pushing after full cervical dilation at the point when the fetal head was in the occiput anterior position and uterine contractions were at least 30 mmHg.

本研究目的在探讨初产妇女第二产程立即用力与延迟用力对产后疲惫及生产结果的影响,收案对象为怀孕38-42周初产妇女、待产期间未施打硬脊膜外麻醉,且怀孕期间无任何合并症,於待产期间向其解释研究目的及收案方法后予以收案,采类实验设计法,以方便取样并依个案之意愿分派於实验组及控制组,实验组於子宫颈完全扩张后等到强烈想用力的感觉、胎头位到达坐骨棘连线下1公分以下、胎头位置转到枕前位、子宫收缩规律强度至少30mmHg才教导向下用力;控制组於子宫颈完全扩张后即教导向下用力,共收案72位。

While according to the data of present and published data, deletion of CtBP-binding motif caused lost of repressive activity accompanied with change of sub-nuclear localization, implicating that a close relationship between them.

CtBP结合Motif的缺失引起转录抑制活性的消失并伴随亚核定位方式的改变,说明BKLF的转录抑制活性与其亚核定位方式之间存在密切的关系。

Twenty tobacco transgenic plants were screened for the expression of the hLf gene: 1g of fresh weight of transgenic leaf tissue was ground in liquid nitrogen and total soluble proteins were extracted. The following figure shows the Western analysis of the total soluble protein extract from transgenic leaves (1. concentrated protein extract; 2. milk hLf. The position of protein standards is shown on the left).

以20棵基因转殖菸草,筛选hLf基因的表现,取一克新鲜的转殖叶片组织於液态氮中研磨,以萃取所有可溶性蛋白,右图显示将这些由转殖叶片萃取之蛋白进行西方墨点分析的结果样本1为浓缩之蛋白萃取液,样本2为牛奶的hLf,而标准蛋白质(marker位於左侧。

Twenty tobacco transgenic plants were screened for the expression of the hLf gene: 1g of fresh weight of transgenic leaf tissue was ground in liquid nitrogen and total soluble proteins were extracted. The following figure shows the Western analysis of the total soluble protein extract from transgenicleaves (1. concentrated protein extract; 2. milk hLf. The position of protein standards is shown on the left).

以20棵基因转殖菸草,筛选hLf基因的表现,取一克新鲜的转殖叶片组织於液态氮中研磨,以萃取所有可溶性蛋白,右图显示将这些由转殖叶片萃取之蛋白进行西方墨点分析的结果样本1为浓缩之蛋白萃取液,样本2为牛奶的hLf,而标准蛋白质(marker位於左侧。

The predicted amino acids are indicated by one-letter code designations below the nucleotide sequence.

BusuNPV p47基因的起始密码子上游第16位有一杆状病毒早期基因转录起始信号CAGT[12],在-100位和-120位有两个早期启动子组成元件TATA。

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We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称;三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起,称之为挽歌体诗人或者史诗诗人,他们被称为诗人,似乎只是因为遵守格律写作,而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。

This is not paper type of business,it's people business,with such huge money involved.

这不是纸上谈兵式的交易,这是人与人的业务,而且涉及金额巨大。