转位

METHODS: After AIF△1-120 gene was successfully cloned, the shorter truncated human AIF gene (AIF△1-400) was constructed by deleting the Nterminal mitochondrial localization sequence, the coding sequence of flavine adenine dinucleotide binding domain and nicotinamide adenine dinucleotide binding domain of AIF protein.The truncated human AIF gene (AIF△1-400) was inserted into the pIRES2EGFP and pcDNA3 eukaryotic expression vectors, and the coexpression vectors were then transfected into HeLa cells with LipofectAMINE.

在AIF△1-120基因克隆成功的基础上进一步改造，构建截去AIF基因线粒体定位信号，FAD结合结构域和NADH结合结构域（1-400位氨基酸）编码序列的AIF△1-400基因，将其克隆入pcDNA3和pIRES2EGFP真核表达载体，用脂质体法转染HeLa细胞，通过荧光显微镜观察，MTT检测等方法检测该基因在转染细胞中的表达及其对肿瘤细胞的促凋亡活性。

METHODS: After AIF△1-120 gene was successfully cloned, the shorter truncated human AIF gene (AIF△1-400) was constructed by deleting the Nterminal mitochondrial localization sequence, the coding sequence of flavine adenine dinucleotide binding domain and nicotinamide adenine dinucleotide binding domain of AIF protein.

在AIF△1-120基因克隆成功的基础上进一步改造，构建截去AIF基因线粒体定位信号，FAD结合结构域和NADH结合结构域（1-400位氨基酸）编码序列的AIF△1-400基因，将其克隆入pcDNA3和pIRES2EGFP真核表达载体，用脂质体法转染HeLa细胞，通过荧光显微镜观察，MTT检测等方法检测该基因在转染细胞中的表达及其对肿瘤细胞的促凋亡活性。

However, the transformation rate from dominant stem to overtopped stem and from overtopped stem to dominant stem were the lowest. The interconversion of classified stem would increase as the stand density and the rate of L. gmelinii was going up. The altitude that classified stem mainly concentrated was less than 1000m, slope was less than 25°, aspect was south slope, position was at middle and lower, soil depth less than 17cm. The rate of no interconversion was 66.7% among overtopped stem. It should cut the overtopped stem as intermediate cutting.

优势木转被压木和被压木转优势木的转换率最低；随着林分密度和林分中落叶松比例增加，分级木相互转换数也增多；分级木转换现象主要集中在海拔＜1000m、坡度＜25°、坡向为阳坡、坡位为中下、土壤厚度＞17cm的林分中；被压木中，无转换的占66.7%，抚育采伐时应考虑伐除这些被压木。

In group 2, except 3 patients (2 in groups 2a, 1 in group 2b) who had morderate limitation of forearm rotation need further management, both pronation and supination could be restored to more than 80. As a result, 37 wrists (92.5%) of 36 patients were satisfied with the operation.

前臂旋转的回复，除了group 2a中的2位因有中等程度的内转限制均为77外，其余group2各组的内、外转皆可达到80以上，而36例37手腕（92.5%）的手术，都能令病人得到满意的结果。

He may be turned from side to supine to prone position.

他可以从侧卧位转成仰卧位或俯卧位。

Real estate transaction market a staff member said that the past can be faster transfer of the mortgage can be transferred from the Four Tael Lee Cheung Sam's name, just like many of the changes to the contract that changes, some people have formed a distance Cooking auction, the high left Dixi, highly competent, and now all handle.

房产交易市场的一位工作人员说，过去期房可以转让，按揭贷款也可以从张三转到李四的名下，许多刚鉴证过的合同说变更就变更，有的炒房者手上攥着十几套期房，高抛低吸，游刃有余，现在统统玩不转了。

Methods Amplify the cDNA sequence encoding truncated HCV gene, with a part of carboxyl-terminus deleted, by PCR. Synthesize the mimic epitope at E2 region of HCV and seven T or Th cell epitope genes of NS3-NS5 respectively. Bind HCV core gene with a part of carboxyl-terminus deleted to the synthetic epitope gene by PCR, then clone into eukaryotic expression vector pcDNA3.1 and transiently transfect COS7 cells.

用PCR方法扩增核心区羧基端部分缺失的基因片段；分别合成HCV E2区模拟表位和NS3~NS5 7个T或Th细胞表位基因；PCR方法将羧基端部分缺失的HCV核心区基因与合成的表位基因串联，克隆入真核表达载体pcDNA3.1，并通过脂质体瞬时转染COS7细胞。

I have only one thing to say: U-turn if you want to.

我只有一件事要说：如果你想大转弯你就转，但这位女士不会转。

This article studied differences in postpartum fatigue and birth outcomes between women who pushed immediately and those who delayed pushing during the second stage of labor. Data were collected from primiparous women in their 38th to 42nd gestational week who did not receive epidural analgesia during labor and were free of complications during pregnancy. Using a quasi-experimental design, 72 participants selected by convenient sampling were assigned based on individual participant's preference to either an experimental or control group. For the experimental group, pushing was delayed until the point after full cervical dilation at which the mother felt a strong physical pushing reflex, the fetal head had both descended to at least the +1 level in the pelvis and turned to the occiput anterior position, and uterine contractions were at least 30 mmHg. For the control group, the physician instructed mothers to begin pushing after full cervical dilation at the point when the fetal head was in the occiput anterior position and uterine contractions were at least 30 mmHg.

本研究目的在探讨初产妇女第二产程立即用力与延迟用力对产后疲惫及生产结果的影响，收案对象为怀孕38-42周初产妇女、待产期间未施打硬脊膜外麻醉，且怀孕期间无任何合并症，於待产期间向其解释研究目的及收案方法后予以收案，采类实验设计法，以方便取样并依个案之意愿分派於实验组及控制组，实验组於子宫颈完全扩张后等到强烈想用力的感觉、胎头位到达坐骨棘连线下1公分以下、胎头位置转到枕前位、子宫收缩规律强度至少30mmHg才教导向下用力；控制组於子宫颈完全扩张后即教导向下用力，共收案72位。

The predicted amino acids are indicated by one-letter code designations below the nucleotide sequence.

BusuNPV p47基因的起始密码子上游第16位有一杆状病毒早期基因转录起始信号CAGT［12］，在-100位和-120位有两个早期启动子组成元件TATA。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。