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The mRNA expression of MMP-2 in more than 4 lymphnodes positive is lower than in lymphnode 1-3 positive (P<0.05).The MMP-2 mRNA expression is irrelated with tissue grade,pathologic type,ER,PR and Her-2.Conclusion MMP-2 mRNA expression is upregulated in early clinical stage,lymph node metastatic early stage,and down regulated with tumor progressing.MMP-2 mRNA expression is related with the metastasis of breast cancer.
MMP-2 mRNA转录水平的表达与乳腺癌转移有关,MMP-2在转录水平的表达在肿瘤较小时上调,随着肿瘤的增其转录水平表达下调,随着肿瘤恶性程度的增加,其转录水平的表达量在下降,在肿瘤发生的早期表达上调,随着肿瘤的进展表达量下调。
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The plant and environment interactions and the molecule mechanism, include light acceptors and its message transductions, heat shock and its protein induced, phytopathological defensive reaction and its elicitors and related proteins, mechanical injury message and the signal introduction.
环境刺激及其反应的分子机制:光受体及其信号转导;热激及热激蛋白、功能与热激反应的信号转导;植物的病理防御反应,激发子及其结合蛋白与防御反应的信号转导;机械与伤害信号分子及其信号转导。
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Methods After the wide type NCI-H446 cells and the three kinds of NCI-H446 cells transfected by three kinds of IL-15 genes respectively (Cmp transfected by the IL-15 maturation peptide gene, Cp transfected by prototypic IL-15 gene, and Csp transfected by modified IL-15 gene whose signal peptide was replaced with the IL-2 signal peptide) were treated by mitomycin, the cells (named MCw, MCmp, MCp, and MCsp, respectively) were used as stimulator cells to stimulate PBMC from healthy volunteers.
野生型NCI-H446细胞和被3种IL-15基因分别转染的3种NCI-H446细胞(Cmp:被IL-15成熟肽基因转染;Cp:被原型IL-15基因转染;Csp:被信号肽换为IL-2信号肽的改型IL-15基因转染),用丝裂霉素处理后(分别名为MCw、MCmp、MCp和MCsp)作为刺激细胞刺激健康志愿者的PBMC。
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The identified proteins are involved in a variety of cellular process including several zinc finger proteins relevant to transcription regulation, such as zinc finger 198, 263, 14, 224, zf6, novel protein similar to transcriptional represser CTCF, and kruppel-like zinc finger protein; two members of the ADAMs (a disintegrin and metalloprotease domain) family; two members of integrin family; several proteins involved in the signal transduction, cell-cycle control, chromatin remodeling and transcription repression; and also some proteins of cell skeleton and some with unknown functions.
鉴定出 的蛋白包括多个与转录调控相关的锌指蛋白,如锌指蛋白198、263、14、224、zf6、转录抑制因子cTcF样蛋白、幻肚pple样锌指蛋白等:两个含金属蛋白酶结构域和整合素结合结构域的家族成员ADAM28和ADAM17;两个整合素家族成员pZ整合素和含十个EGF样结构域的整合素;与细胞信号转导通路有关的蛋白;与细胞周期调控有关的蛋白;与染色体重塑和基因转录抑制有关的蛋白;细胞骨架蛋白以及其他功能未明的蛋白等。
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Part II The experiment study on anti-tumour effect of dendritic cells derived from peripheral blood monocyte of patients with oral cancer after pulsed by tumour antigen in different ways. Collected 50ml peripheral blood from each patients and volunteers to induced DC in vitro. We parted the test group and control group into three subgroups respectively, untreated group A, freezed and thawn group B, RNA transfected group C. To group A, after induced DCs for five days , we added 200U/mlTNF-α in each hole and cultured on. To group B, we added protein antigen (DC/ tumor cells = 1 : 1 ,each hole) for 4h at 5d, and then added 200U/mlTNF-α in each hole and cultured on. To group C, transfected immature DC with tumor cell RNA(DC:RNA=1×105/ml: 5ug)at 5d, operated completely with the specification of invitrogen DMRIE-C reagents. After transfection we replaced transfection agent with complete medium, and then added 200U/mlTNF-α in each hole and cultured on.
第二部分口腔癌患者外周血来源的树突状细胞体外负载抗原后抑瘤作用的实验研究无菌采集20例口腔癌患者和20例健康志愿者外周血50ml,分离单个核细胞体外诱导培养DC,并将实验组和对照组细胞各分为三组,A组为未处理组,B组为冻融组,C组为RNA转染组。A组DC在培养5d后,每孔加入TNF-α200U/ml;B组DC培养第5d后,每孔中加入癌细胞蛋白抗原,使DC/肿瘤细胞=1:1,继续培养4h后,每孔加入TNF-α200U/ml;C组将培养至第5d的未成熟DC用肿瘤细胞RNA进行转染(DC:RNA=1×105/ml: 5ug,按照invitrogen DMRIE-C试剂说明书进行操作),转染结束后用完全培基取代转染剂,每孔加入TNF-α200U/ml。
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A single dose of albendazole 400 mg was used as control. Results The egg negative conversion rates of ivermectin and albendazole were both 100%(34/34) for Ascaris infection, 17.6%(6/34)and 76.5%(26/34)respectively for hookworm infection, 67.6%(23/34)and 47.1 %(16/34)respectively for Trichuris infection,58.8%(20/34) and 100%(34/34)respectively for Enterobius infection.
结果随机双盲治疗,伊维菌素和阿苯达唑对蛔虫感染的虫卵阴转率均为100%(34/34),对钩虫感染的虫卵阴转率分别为17.6%(6/34)和76.5%(26/34),对鞭虫感染的虫卵阴转率分别为67.6%(23/34)和47.1%(16/34),对蛲虫感染的虫卵阴转率分别为58.8%(20/34)和100%(34/34)。
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The activities of C4 photosynthesis enzymes in untransformed rice were very low,while those of corresponding enzymes in different transgenic rices were all highly expressed.
在高光条件下,与原种相比较,转PPDK基因水稻的光合速率未增加;转ME基因水稻的光合速率降低了6.1%;转PEPC+PPDK双基因水稻与转PEPC基因水稻相近。
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Regarding the second issue, I will compare the semantic meanings of various autonyms used by all major subgroups who are generally referred under the Chinese transliteration "苗族" in China and the English transliteration "Hmong" outside China. Neither the Chinese "苗族" nor the English "Hmong" has been a perfect transliteration of any subgroup autonym of the Hmong People proper, in spite of the fact that both of them partially reflect some semantic meanings of these autonyms.
关于第二个问题,我将对中国&苗族&这个汉文通称转写形式下和境外&Hmong&这个英文通称转写形式下所包涵的各个不同方言支系自称及其语义作一个比较分析:尽管这两种外来语言对苗族的统称转写形式,多多少少也可能与一些苗族支系自称的语义有着某种联系,但是,其实无论是用&苗族&还是用&Hmong&作为统称转写形式,对任一苗族自称形式而言,都不是理想的表达。
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Material is fed through a feed pipe, distributed evenly on the filter screen wall of the screen-bowl bottom.
电机带动转鼓及同心安装的输料螺旋,以一定的差速同向高速旋转,物料由进料管连续引入,均匀分布到转鼓底段滤网壁上,在离心力作用下,液相物穿过滤网和转鼓壁滤孔排出转鼓,经排液口排出机外。
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Based on the above actuality, this paper studied the high quality incineration technics and theory in a self-designed rotary incinerator. The variation of several operation conditions such as waste feedrate, excess air ratio, air distribution and rotation speed of the fulcra has been employed to study the formation and control theory of NOx, SO〓and HCl. Based on the experimental results, a simple and comprehensive model has been proposed to describe the process of acid gas formation and reduction. The results show that the formation of pollutants strongly depend on the incineration state of the solid waste in the rotary incinerator.
本研究在—自行设计的新型转式垃圾焚烧炉中,重点研究了焚烧炉的优质焚烧工艺和理论,并在此基础上,研究模化有机垃圾在转式焚烧过程中酸性污染气体NOx,SO〓和HCl的生成控制原理,特别是研究进料率、过剩空气系数、配风和转轴转速等条件对NOx,SO〓,HCl生成的影响,并在此基础上建立了转式垃圾焚烧炉中酸性污染气体的生成理论模型,结果表明,焚烧过程污染物的生成强烈地依赖于垃圾在转式焚烧炉内的燃烧状态,必须在高垃圾燃烬率和低污染物排放之间寻找优化的平衡条件。
- 推荐网络例句
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Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.
在对危险的南部地区访问时,他斥责什叶派民兵领导人对中央集权的挑衅行为。
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In fact,I've got him on the satellite mobile right now.
实际上 我们已接通卫星可视电话了
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The enrich the peopling of Deng Xiaoping of century great person thought, it is the main component in system of theory of Deng Xiaoping economy, it is a when our country economy builds basic task important facet.
世纪伟人邓小平的富民思想,是邓小平经济理论体系中的重要组成部分,是我国经济建设根本任务的一个重要方面。