转

Transcription/translation assay and transient transfection of COS-7 cells with a cDNA encoding Sparus aurata lier GK showed the functionality of the enzyme in itro.

转录和翻译水平的分析，以及用金头鲷肝脏GK的cDNA转染COS－7细胞都表现出GK在体外的活性。

A cognitive study of its semantic extension, in light of the metaphor and metonymy theories in cognitive semantics, reveals that its meaning extends and changes in a spokewise fashion interlaced by a concatenate pattern, in which metaphorical and metonymic mappings are interwoven, and that metaphor and metonymy are two cognitive approaches to its semantic extension.

依据认知语义学中的隐喻和转喻理论对&兵&的语义扩展认知机制进行分析，可以发现，在&兵&的语义扩展的过程中，语义的辐射型变化交织着连锁型变化，隐喻和转喻从中共同发挥作用，它们是其语义发展的两条主要认知途径。

Time is like a wheel, turning and turning, never stoping.

时间像个轮子，转啊转啊，永不停息。

It has been demonstrated that STAT3 is activated after cerebral ischemia/reperfusion in cortex and striatum.

以往的研究表明，在脑缺血/再灌注的皮层和纹状体组织中信号转导与转录激活子-3 (STAT3)被激活。

After changing medium, BMSCs were incubated in serum-free DMEM containing BRA and dimethyl sulphoxide for 5 hours to 6 days. MAIN OUTCOME MEASURES: Cell transfection was observed with a fluorescence microscope.

主要观察指标：荧光显微镜观察细胞转染情况，免疫细胞化学染色检测转染细胞诱导后神经元表面抗原的表达。

The derivatives of the amino acids containing sulphur synthesizedfrom three sulphydry amino acids such as methione, cysteine and cystine play an important role on the methylation,sulphuration, detoxication and ,antioxidation and are very useful in pharmaceutic and food industry.

含硫氨基酸衍生物主要由三个常见的含硫氨基酸——蛋氨酸，半胱氨酸，胱氨酸衍生转化而来，可以在体内参与转甲基，转硫基等生化过程，同时也具有抗氧化，清除自由基等功能，在医药和食品中具有重要的应用价值。

"You're right." He swiveled back around and closed the laptop he'd been working on then quickly swiveled back towards Cameron again.

&你说得对。&他把椅子转了回去，合上了笔记本电脑，又马上转了回来。

Ansochromogenes was transformed by pUC1169 from itself. 8 mutants lost ability to produce nikkomycin were screened from 4068 colonies of Vio〓 Thio〓 with Tn4560 transposition. Southern blotting suggested that Tn4560 was inserted in at least 4 positions of chromosomal DNA in S.

于39℃，MM／Vio条件下培养携带有pUC1169的7100孢子，Tn4560发生转座；筛选到4068个转座菌落，并从中得到8株尼可霉素阻断突变株；对这8株突变株的总DNA进行Southern杂交分析表明，Tn4560至少在4个不同的位点插入到7100的染色体上。

The efficiency of selection was monitored by comparing the number of phage recovered from the acid elution and cell lysate in each round,and by testing EGFR binding specificity of polyclonal phagescFv on CHOEGFRGFP1 and CHOK1 cell with cell ELISA. Bacterial PCR was used to select clones containing a 1 kb insert. Cell ELISA was used to determine EGFR binding specificity of monoclonal pscFv on EGFR positive and negative cell. The number of individual EGFRbinding clones was determined with nucleotide sequencing. Results 500fold enrichments were observed by tittering phages in the cell lysate after five rounds of selection.

以稳定转染的CHOEGFRGFP1细胞和未转染的CHOK1细胞分别作为EGFR阳性和阴性细胞，采用负筛选的方法进行筛选；通过比较每轮投入及洗脱出噬菌体的效价比以及细胞ELISA检测多克隆pscFv与阳性、阴性细胞结合情况对筛选过程进行监测；采用菌落PCR挑选含有全长scFv片断的菌落，进一步用细胞ELISA检测单克隆pscFv与EGFR阳性及阴性细胞结合特异性；挑选EGFR特异性单克隆pscFv采用DNA测序法确定克隆多样性。

Cell ELISA was used to determine EGFR binding specificity of monoclonal pscFv on EGFR positive and negative cell. The number of individual EGFRbinding clones was determined with nucleotide sequencing. Results 500fold enrichments were observed by tittering phages in the cell lysate after five rounds of selection.

以稳定转染的CHOEGFRGFP1细胞和未转染的CHOK1细胞分别作为EGFR阳性和阴性细胞，采用负筛选的方法进行筛选；通过比较每轮投入及洗脱出噬菌体的效价比以及细胞ELISA检测多克隆pscFv与阳性、阴性细胞结合情况对筛选过程进行监测；采用菌落PCR挑选含有全长scFv片断的菌落，进一步用细胞ELISA检测单克隆pscFv与EGFR阳性及阴性细胞结合特异性；挑选EGFR特异性单克隆pscFv采用DNA测序法确定克隆多样性。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。