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In order to do this, we get equal quantities of plasmid pSCA1-M, pcDNAM and pSCA1 transfected vero cells. After 48 hours, we observed the presence of special green fluorescence in cytolymph in both of pSCA1-M and pcDNAM groups. However, this fluorescence is absent in the negative pSCA1 group.

分别取等量的重组质粒pSCA1-M、常规IBV M基因DNA疫苗质粒pcDNAM、空载体pSCA1转染Veto细胞。48h后经间接免疫荧光试验观察发现，pSCA1-M以及pcDNAM转染组细胞浆均可见特异性亮绿色荧光，pSCA1空载体组无荧光为阴性。

MAIN OUTCOME MEASURES: Transfection rate and effect of ECV304 proliferation were calculated by fluorescence microscope and flow cytometer.

主要观察指标：①观察转染后对ECV304生长的影响，在流式细胞仪和荧光显微镜下计算转染效率。

Methods Westernblot was employed to detect the intracellular expressions of caspase-9, hTERT, Bcl-2 and Bax, and mitochondrial and cytoplastic cytochrome C in HepG2 cells transfected by pSliencer 3.1-H1 neo-shTERT.

收集第20代hTERT基因RNAi真核表达载体论定转染细胞、对照细胞及未转染HepG2细饱，采用Western blot检测线粒体，胞质细胞色素C的表达及细胞内caspase-9、Bcl-2、Bax和hTERT的表达。

The false operation and the operation of removal pineal gland were carried out at 1 month before the Winter Solstice and the Summer Solstice each year, rats were bred after the operation and were decollated at night on the Winter Solstice and the Summer Solstice.

2.2 实验研究：(1)总体设计：本实验研究主要以与&肾应冬，主生殖&相关的性腺轴和对光节律极为敏感的松果腺为切入点，选择了在细胞信号转导途径中起重要作用的G蛋白和受体分子，以及在细胞信号转导末端的基因表达作为研究的对象。

The demethylation of DNA and acetylation of core histone reconstitute chromosome struction which is beneficial to gene transcription.

DNA去甲基化与组蛋白乙酰化导致染色质构象发生有利于基因转录的变化，基因具有转录活性。

Objective Establishment of a hybridoma cell line secreting a monoclonal antibody to facilitate iˉdentification of human tissue kallikrein（KLK1gene,hK1protein）.Methods Mice were immunized with E.coli-exˉpressed GST-hK1fusion protein and their spleen cells were fused with SP2/0myeloblastoma cells.Specificity of the monoclonal antibody was shown by Western blotting and by immunofluorescence.Results The monoclonal antibody reacted specifically to E.coli-expressed hK1and with the KLK1cDNA-transfected COS-1cells.

目的 将人组织激肽释放酶（基因命名 KLK1，酶命名为hK1）cDNA克隆入肠杆菌表达质粒，以重组菌表达的GST-hK1融合蛋白免疫小鼠，获得了分泌hK1特异单克隆抗体的杂交瘤细胞株方法将KLK1cDNA克隆入真核表达质粒，用获得的重组质粒转染COS-1细胞，经间接免疫荧光试验证明，上述单克隆抗体能与转染细胞发生特异反应。

When sprag-type clutches are used in hoist and derricking systems, they shall incorporate a positive mechanical lock against failure or be designed to transmit twice the maximum torque imposed by the maximum line pull.

提升和变幅系统使用斜撑离合器时，离合器必须带有一个可靠的机械锁，以防出现故障。离合器的设计应当可以传递最大直线拉力产生的最大转矩的两倍的转矩。

Methods The IL17 cDNA and Thy1.1 fulllength cDNA were subcloned into MIT(MSCVIRESThy1.1) retrovirus vector, and the siRNAIL17, U6 promoter and Thy1.1 fulllength cDNA were also inserted into retrovirus vector of pMNDBANSHEE.The recombined vectors were transfected 293 packaging cells by DNA calcium phosphate coprecipitation. Virus supernatant which infected preactivated spleen cells from NOD/BDC mice was collected. After incubation, the IL17 expression in diabetogenic T cells was detected.

利用基因工程和细胞克隆技术分别将IL17的cDNA插入MSCVIRESThy1.1逆转录病毒载体，将Thy1.1、U6增强子(U6 promoter)基因和IL17的siRNA cDNA插入逆转录病毒载体pMNDBANSHEE，采用磷酸钙沉淀法将重组载体转染293包装细胞；收集病毒上清，转染预先活化的NOD/BDC小鼠脾细胞，测定致糖尿病性T细胞的IL17和siRNAIL17的表达。

Daier that discontenting experiencing people to this nobleman family, one fine day may be done , he is unwilling to be that this family bears this responsibility , he is turned to a bourgeoisie by nobleman class, fairly more different than exploiting class turns to a proletariat.

代尔那感受到人们对这个贵族家庭的不满，总有一天会完蛋，他不愿为这个家庭承担这个责任，他由贵族阶级转到资产阶级，还不同于剥削阶级转到无产阶级。

To investigate the expression, location and function, long distance PCR was used to expand the targets containing open reading frame. The expressive vectors of prokaryotic and eukaryotic cells were constructed after product purification and connecting with vectors. The prokayotic vectors were induced to express the protein by IPTG and the protein was seen by denaturalization PAG electrophoresis and dyeing. The eukayotic expressive vectors were transfected into culture cells and the protein was found in the nulceolus under the observation of the confocal microscope. The transfected cells were chosen by the geneticin (G418). The cell cyele was examined by flow cytometer and the cell numbers were reduced obviously in the stage of G〓-G〓 and G〓-M, but increased distinguishably in the S stage.

为研究磷酸化应激诱导蛋白的表达、定位和功能，采用了长距离聚合酶链反应扩增含有开放阅读框架的靶片段，经产物纯化、与载体连接，分别构建了STIP1基因的原核表达载体和真核表达载体；IPTG诱导原核表达载体，变性聚丙酰胺凝胶电泳与染色后，可见相应大小的蛋白质表达；STIP1基因的真核表达载体转染细胞系后，共聚焦显微镜下观察STIP1蛋白主要分布于细胞核内；用药物筛选STIP1基因转染的细胞后，流式细胞仪检测细胞周期，可见G〓-G〓期和G〓-M期的细胞明显降低，S期细胞明显增多。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。