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Methods The skeletal muscle stem cells were enzymatically isolated with collagen and dispase, and then purified by different adhesion time from 6 female SD rats. The purified cells were transinfected with adenovirus carrying lac-Z gene, and injected into the bladder neck of 6 autologous and 4 allogeneic rats. Tissues of blader neck and ureter were obtained in sacrified rats after 5 and 15 days, histological examination and X-gal staining were performed.

采用混合酶消化及反复差速贴壁法分离、纯化培养SD大鼠骨骼肌干细胞,然后用携带lac-Z基因的腺病毒载体染肌干细胞,待染成功后将携带lac-Z基因的骨骼肌干细胞注射于自体和同种异体的膀胱颈及后尿道周围,分别于注射后5、15d,处死大鼠取出膀胱颈及后尿道,行组织学检查及X-gal染色。

Through determining leaves of Bt transgenic tobacco and leaves, flowers, bolls and bracteal leaves of Bt transgenic cotton, it is found that ELISA method is relevant to bioassay.

用建立的ELISA方法检测了Bt杀虫蛋白基因烟草的叶片和Bt杀虫蛋白基因棉花的叶片、花器、铃和蕾等部位,并同生物测试法相比较,发现两种方法有很好的相关性。

Parametering by adsorption in transfer foil and plastic injection into the mold cavity, the combination of the predeposited design will go to the cancellate, the design will be able to make precise positioning, and the finished product and do not need additional post-processing, such as jet protectors, and so on.

通过吸附在注塑模腔的印箔膜和注进模腔熔胶的结合,预涂的设计图案便附在凝固的塑件上,该设计图案更可以作出精密定位,而成品也不需要额外的后期处理,如喷保护漆等。

Benign prostatic hyperplasia patients with a huge prostate and those with intraoperative slit of capsula prostatica undergoing TURP are more likely to be converted. And uncontrollable hemorrhage, huge prostate and poor endoscopic vision are the major reasons for the conversion.

前列腺体积巨大和术中切透前列腺包膜是TURP开放手术的危险因素;术中无法控制的出血、手术视野不清和前列腺体积大是TURP开放手术的主要原因。

Thirty-eight of them were male patients and 13 were female. The results of arthrography were normal in 21 cases, impingement in 8 cases, rotator cuff tears in 6 cases, adhesive capsulitis in 5 cases, and rotator cuff tear combined with either impingement or adhesive capsulitis in 11 cases.

病人的平均年龄是60岁(23至77),38位是男性,13位是女性,肩关节双重显影摄术的结果如下列:21例是正常,8例是撞击症候群,6例是轴肌断裂,5例是粘黏性肩关节炎,另11例是轴肌断裂合并有粘黏性肩关节炎或撞击症候群。

Methods Fourteen healthy pigs were anesthetized and intubated, the right carotic artery was cannulated for blood pressure monitoring and blood biochemical sampling.

术中输入不含糖的平衡盐液,供肝用Collin's液灌注。在无肝期采用体外静脉流,经左侧股静脉和门静脉插管,引流出的血流经体外流系统输入左侧腋静脉。

Methods A follow-up survey of 3-4 months(average 20.36 ±5.35 months) to 56 simple obe- sity case was castled out.

追踪肥胖的归对防治肥胖尤为重要。方法为了解肥胖的归,本文对56例单纯性肥胖患者的体重。

Through frequent contact with animals, human beings have recognized the categorial attributes of animals. By means of rhetorical devices, such as metaphor and metonymy, these attributes of the animal category are mapped into other categories because of their relevance or similarity.

在与动物的频繁接触中,人类认知了动物的不同种属特性,这些特性因为与其他事物或概念有关联性或相似性而联系起来,通过隐喻或喻模型,映射到其他事物的种属特性中,使动物词语指、表达其他事物或概念。

The upper and lower epidermal in the yellow leaf of Celtis julianae 'Aurea' had almost no chloroplast, but after reverting green the chloroplast appeared and the number of the chloroplast, starch grains increased and plastoglobulus reduced.

金叶珊瑚朴黄色叶的上下表皮细胞中几乎不含叶绿体,但绿后的叶片中上下表皮叶绿体的数目增多,故叶色变绿。叶色由黄绿过程中,淀粉粒增多,体积变大,嗜锇小球减少。

Using the transfer matrix method, calculation problem of the spin tube gun's vibration characterist...

本文建立了管炮的发射动力学模型,应用多体系统传递矩阵法,解决了管炮在高速旋情况下的振动特性的计算问题。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

聚光:照片上是建在西班牙桑路卡拉马尤城的一座新型PS20塔式太阳能电站。被称为&日光反射装置&的镜子将太阳光反射到主塔,然后用聚集的热量产生蒸汽进而通过涡轮机转化为电力