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Compared the lignin and cellulose level of these two kinds of transgenic tobacco, we found that the cellulose content increased and the lignin content deduced in transgenic tobacco transformed with Amorpha fruticosa UGPA constructs, both the cellulose content and the lignin content increased in transgenic tobacco transformed with Acetobacter xylinum UGPA constructs.

结果表明，两种转基因烟草的尿苷二磷酸葡萄糖焦磷酸化酶的活性均有明显提高；转紫穗槐UGPA基因的烟草纤维素含量有所提高，木质素含量略有降低，纤维素与木质素含量之大连理工大学博士学位论文比提高；而转木醋酸菌来源的UGPase基因的烟草木质素含量和纤维素含量均显著提高。

The pcDNA〓-apoE〓 plasmid was transfected to SK-N-SH Neuroblastoma cell by liposome. It could be screened by G〓. The immunohistochemical assay was shown that neuroblastoma cell transfected by pcDNA〓-apoE〓 expressed the recombinant apoE protein. The intracellular expressed recombinant protein could inhibit the death of neuroblastoma cell induced by beta amyloidal peptides 25-35 fragment.

用pcDNA〓—apoE〓真核表达载体与脂质体共转染神经成纤维胶质瘤细胞，用G〓选择压力筛选，细胞免疫荧光检测表明，pcDNA〓—apoE〓转染的神经成纤维胶质瘤细胞表达了apoE〓重组蛋白，这种内源性的apoE〓重组蛋白对25μM Aβ25—35多肽诱导的神经成纤维胶质瘤细胞的死亡具有拮抗作用。

The venous catheter has an effective anticoagulative function, and is easy to manipulate.

自制腹腔内静脉转流管抗凝性能优良，操作简便，转流过程中动物容易管理。

METHODS AND RESULTS: Using both primary peritoneal macrophages and studies in advanced atheromata in vivo, we introduce signal transducer and activator of transcription-1 (STAT1) as a critical and necessary component of endoplasmic reticulum stress/type A scavenger receptor-induced macrophage apoptosis.

方法和结果：通过使用原代腹膜巨噬细胞研究在体脂质斑块，我们引入信号转导子和转录激活子-1(STAT1)，作为内质网应激/A型清道夫受体诱导的巨噬细胞凋亡中关键和必要组分。

After the identification of SARs in Attacus ricini rRNA gene, its rRNA gene and NTS were also found to have the ability binding with the nuclear scaffold of Saccharomyces cerevisiae. The 1kb NTS fragment which can bind clearly with nuclear scaffold of Attacus ricini was the chief fragment binding with yeast nuclear scaffold. This indicated the homology between this two kinds of SARs and provided an evidence for the evolutionary conservation of SARs.

在鉴定蓖麻蚕rRNA基因中具有骨架结合区域之后，利用体外结合的方法发现，蓖麻蚕rRNA基因及其非转录间隔区片段也具有与酵母核骨架结合的能力，尤其是能与蓖麻蚕核骨架结合的非转录间隔区1kb片段，也能明显地结合于酵母核骨架，这一结果表明二者的SAR顺序具有一定的同源性，为骨架结合区域具有进化保守性提供了又一证据。

Ingestion of bacterially expressed dsRNAs can produce specific and potent genetic interference in Caenorhabditis elegans.

考虑主要是由于分裂后的原代培养神经元转染效率低，且转染本身对神经元就是一个细胞毒性作用所致。

The air trapping volume had an obvious correlation with FEF50% and MMEF(X^2=10.48, P.01). Conclusions: The CT manifestations of prognosis are demonstrated later than bacteriology. There is a limitation in assessing the activity of pulmonary tuberculosis only by CT signs. CT plays a role in demonstration of secondary small airway changes after bacteriologically cured.

肺结核CT征象转归滞后于细菌学转归，仅凭CT征象对治疗后肺结核活动性判定存在限度；CT对肺结核细菌学治愈后的继发小气道病变的评价有较高价值。

Next,three aspects were considered as key technology and research challenges of bascule bridge deck surfacing here,development on light paving material and research on thin deck surfacing system,numerical simulation of the mechanical properties of bascule bridge deck surfacing based on long cantilever structure and contact behaviors between interfaces,bonding layer material testing.

在介绍国内外立转开启式桥梁桥面铺装研究现状的基础上，从轻质铺装材料开发和薄型铺装结构研究、基于大悬臂结构和界面接触特性的力学性能数值模拟、黏结层材料试验3个方面阐述了立转开启式桥梁桥面铺装研究的关键技术和难点。

The mouse embryonic stem cells cDNA library was screened by yeast two-hybrid assay,some candidate molecules were get,including DDX39 DEAD (Asp-Glu-Ala-Asp box polypeptide 39, BAT1A(HLA-B-associated transcript 1A), NAC1 ( nucleus accumbens-1 ), BicD2 (bicaudal D homolog 2)、MLL3 (myeloid/lymphoid or mixed-lineage leukemia 3). NAC1 and MLL3 were associated with regulation of gene transcription. DDX39 andBAT1A were associated with pre-mRNA processing and mRNA export from nucleus to cytoplasm. BicD2 was associated with the regulation of Golgi body.

通过利用酵母双杂交方法，以小鼠全长Plk1作为诱饵蛋白筛选小鼠胚胎干细胞cDNA文库中与其相互作用的蛋白，获得了DDX39DEAD(Asp-Glu-Ala-Aspbox polypeptide 39、BAT1A(HLA-B-associated transcript 1A)、NAC1(nucleus accumbens-1)、BicD2(bicaudal D homolog 2)、MLL3(myeloid／lymphoid or mixed-lineage leukemia 3)等几个候选蛋白，其中NAC1、MLL3与基因的转录调控有关，DDX39、BAT1A与mRNA前体的剪切、加工及mRNA从细胞核转运到细胞质紧密相关，而BicD2与高尔基体的调控紧密相关，提示我们Plk1与基因转录、mRNA前体的剪切、加工、mRNA的运输及胞质分裂中高尔基体的调控有关。

On strength computation,the finite element models of rotating mirror were established according to the real structure of the mirror,and the rotating three-faced beryllium mirror were analysed.Results display that the surface deformation magnitude of the mirror is approximately 0.334 6 μm,and the maximum stress is 0.204 GPa at 600 m/s of edge linear speeed.The regions of maximum stress are on the axial hole and symmetric to symmetrical planes of mirror body,and keep beelines to surfaces.On the modal analysis,the results show that the first and second modals of bending vibration exist around 3.4×105 r/min and 6.6×105 r/min,which appear binate and orthogonal respectively,and another modal belongs to the first torsional vibration that occurs by 5.13×105 r/min.

运用该方法得出转镜镜面变形量、内部应力分布和振动模态参量的定量数值：在边缘线速度600 m/s下，三面体铍转镜的最大变形量为约0.334 6 μm；最大应力0.204 GPa，位于轴孔附近并与镜面距离最短处；两个在互相垂直平面内的一阶弯曲振动模态发生在3.4×105 r/min附近，一阶扭振点位于5.13×105 r/min附近，两个二阶弯曲振动点位于6.6×105 r/min附近。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。