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ARID (AT-rich interaction domain) protein is a transcription factor family in higher eukaryotes that regulates cell proliferation, development, and differentiation. Specificity of DNA binding ability in this family prefers AT-rich sequences, but some ARID family proteins are not sequence-specific DNA-binding proteins or they do not bind AT-rich sequences. We found two genes that encode ARID in Giardia lamblia genome database, garid1 and garid2. We analyzed the function of garid1 first. AU1-tagged gARID1 was found to localize to nuclei. During encystation, gARID1 mRNA level decreased emphatically, but protein level increased. We also found that gARID1 can bind AT-rich initiator of the cwp1 promoter by EMSA. Mutation analysis revealed gARID1 binding sequence was AGATC and AATAAAATA. We used ChIP to demonstrate that gARID1 can bind cwp1 gene promoter in vivo.

ARID(AT-rich interaction domain)蛋白质家族是真核生物的一种转因子，在许多同种的真核生物有它的同源基因，这个家族的蛋白质通常与调控细胞的生长、发育和分化的作用有关，而这个家族的蛋白质和DNA的结合能，各种ARID蛋白质的专一性尽相同，过大致上偏好於和AT-rich的序结合；我们已经在形鞭毛虫的基因组中找到个含有ARID 的基因，分别是garid1和garid2，我们首先对於garid1做分析；将AU1标记接到gARID1转染形鞭毛虫，用免疫萤光染色可发现gARID1存在於细胞核中。gARID1的讯息RNA在囊体化后会明显下，过其阳性染色和蛋白质表现有明显增加；EMSA实验中也发现gARID1会明显的与cwp1基因启动子之AT-rich initiator结合，经由突变序分析，也显示gARID1的结合序为AGATC和AATAAAATA，随后我们也用ChIP证明gARID1在细胞内也的确会和cwp1基因的启动子结合。

Identification of differently expressed genes between normal liver tissue and liver cancer,can provide valuable candidate genes for prevention,diagnosis and therapy of liver cancerBased on phenotype analysis after gene knockout,endoblast transcription factor HNF6 may regulate mesoblast transcription factor Foxfl.

基于基因敲除后的表型分析及其研究，发现内胚层转录因子HNF6调控中胚层转录因子Foxfl的表达，进而通过调控Foxfl的下游基因Notch2的表达而调控胆管的发育和生长。

METHODS: Tum5 gene was amplified from plasmid pSPORT1Sfi by PCR technique and subcloned into the expression plasmid of lentiviral vector, pGCFU, to generate the lentiviral expression vector, pGCFUTum5. The correct Tum5 gene was confirmed by endoenzyme digestion and sequencing. Recombinant lentiviruses were produced by 293T cells following the cotransfection of pGCFU Tum5 and packaging plasmids-pHelper1.0and pHelper2.0. The resulting recombinant lentiviruses (GCFUTum5) which carried Tum5 and EGFPgene were then used to infect human umbilical vein endothelial cells.

采用PCR技术从含有tumstatin基因的质粒克隆模板 pSPORT1Sfi钓取Tum5基因，并将基因克隆到慢病毒载体表达质粒pGCFU中，构建慢病毒载体表达质粒pGCFUTum5，通过酶切、测序验证Tum5基因后，将pGCFUTum5质粒和包装质粒pHelper1.0，pHelper2.0共同转染人胚胎肾上皮细胞系293T细胞，获得携带Tum5基因和EGFP基因的重组慢病毒GCFUTum5，并转染靶细胞人脐静脉血管内皮细胞。

The result of our study shows that vertigo in AAT possible had assemble pathology of endolymph hydros which proven indirectly by the change of ECoG expect the direct injury of the vestibular system or perilymph fistule.

本研究结果显示急性听性外伤造成的眩晕，除了前庭迷路系统的直接伤害或外淋巴瘻管，有可能类似内淋巴水肿的病理机转，此病理机转可藉由耳蜗电图的变化而间接加以证实。

Results: among the 40 cases of patients, 33 cases were early and middle pregnant stage, 7 cases were late pregnant stage. 38 cases had lower abdominal pain, 40 cases had abdominal tenderness and enteron symptom, 33 cases had fever. 25 cases were operated on after diagnosis and all were cured, 1 case occurred incision infection, 1 case occurred abortion. 15 cases received conservative treatment, 12 cases were early and middle pregnancy, 5 cases were cured, 4 cases were improved, 3 cases failed in conservative treatment and operated on surgery, 3 cases occurred abortion, among them, 1 case had incision infection. 3 cases were late pregnant stage, all were operated on, 3 cases occurred premature birth, and 2 cases occurred premature death.

结果：40例患者中，处于妊娠早、中期33例，晚期7例。38例表现为右下腹痛，40例均有腹部压痛及消化道症状，33例伴发热。25例诊断后立即手术者均治愈，术后切口感染1例，流产1例。15例行保守治疗，其中，早、中期妊娠12例，治愈5例，好转4例，保守治疗失败3例症状加重转手术治疗，3例均流产，其中1例合并切口感染；晚期妊娠3例，均症状加重转手术治疗，3例均早产，2例早产儿死亡。

In blastula embryos and 50% epiboly embryos, the zygotic pou2 transcript is also distributed in every blastomere cell. At the stage of 90% epiboly, expression of pou2 converges to the dorsal midline of embryos and locates in two waves of lateral neural plate and two transverse stripes in the brain region. In embryos at 100% epiboly, pou2 transcript is restricted in the neural plate and areas of presumptive midbrain amid himdbrain. From somite stage, gibel carp pou2 can not be detected any more, which is consistent with the RT-PCR result.

在高囊胚期和50%下包期，高度表达的合子转录本仍在所有的胚盘细胞中，但至90%下包期时，pou2的表达向胚胎背部的正中线汇聚，集中在神经板的两侧区域和脑部的两条横向条带在100%下包期时，pou2的表达集中在神经板的中间区域以及预期形成的中后脑区域至体节期时，转录本消失，这与RT-PCR结果高度一致。

Epictetus likewise argued that "men are disturbed not by things, but by the views they take of things."

比拟前人，爱比克泰德有话："人不为外物所转，但为他自己心中之物所转。"

The shorter internodes and out-growth of anxillary buds found in antisense LeETR2 and leaf epinasty observed in antisense LeETRl plants are phenotypes consistent with the negative regulation model of ethylene.

5植株衰老和叶片脱落延迟，花瓣脱落也明显的延迟，在转反义LeETR1基因番茄的果实成熟特性被明显改变，表现在乙烯释放高峰延迟，果实颜色出现变异，以及和成熟相关的酶活性的改变，然而，转反义LeETR2基因的果实的成熟特性与对照相比无明显差别。

There were 90% of nasal and sinuous cavity brought to stage of complete epithelization, 11 to 14 weeks were need in about 60% of them. Conclusion Three stages were divided after FESS.

将术后粘膜转归过程划分为3个阶段：术腔清洁阶段、粘膜转归竞争阶段和上皮化完成阶段。

"This is an important point," Dr. Sawyers told Medscape in an interview,"as it shows that the drug target continues to mutate and that BCR-ABL is the escape mechanism." Hence, further drug development should continue to focus on BCR-ABL and not on any of the other mechanisms that are thought to also be involved, he commented.

Sawyers医师在一项访谈中向Medscape表示，这是个重点，当这项研究显示药物的标的持续在突变，且BCR-ABL基因有逃脱机转，他评论，之后的药物研发应该继续针对BCR-ABL且不应针对其他被认为牵涉其中的机转。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。