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Three RNAi target sites (named as Abil1, Abil2, Abil3) targeting the E3B1 (NCBI: NM-024397), an identified target sites and positive control GAPDH-A were selected. The pGenesil-1 eukaryotic expression vectors with the neoR mark and GFP green fluorescent mark were selected to construct E3B1 RNAi plasmid. The effect of RNAi targeting various sites on E3B1 genes expression was evaluated by testing the transfection efficiency with fluorescence microscope and western blot. The most effective siRNA in inhibiting E3B1 genes were screened. The most effective siRNA in inhibiting E3B1 genes screened and used to transfect the neuronal cells. After that, the inhibitor of axon growth was added and the axon growth was observed. Result: The siRNA expressing plasmids targeting the E3B1 were constructed successfully.

选择针对E3B1(NCBI： NM-024397)RNAi的3个靶位点Abil1、Abil2、Abil3和1个不针对任何mRNA的RNAi靶位点以及甘油醛－3-磷酸脱氢酶，用带有neoR选择标志和GFP绿色荧光标志的真核表达载体pGenesil-1构建E3B1 RNAi质粒，分别转染培养的神经元，在荧光显微镜下观测转染率，经G418筛选得到单一的转染细胞，并用Western blot法检测各转染组神经元E3B1蛋白的表达情况，选出具有最佳抑制效应的siRNA转染神经元，加入轴突生长抑制物，观测轴突生长情况。

ABSTRACT Aiming at problems of trans-vector controller in a vehicle-carried induction motor such as variable rotor and stator resistance following motor temperature, and their inductance varying with flux saturation degrees. Firstly, CSI and VSI are introduced, for CSI, it can hold the advantages both FOC and DTC using stator flux directional, PI parameter and simulation are also introduced. Secondly, equations of IM in the synchronous rotary frame and its static counterpart, as figuring in the saturation condition of magnetic field, are dealt in this paper and the simulation results are presented. Thereafter, the parameter designing of PI trans-vector controller is processed, which features the non-linear model. In practical realization, as neglecting the dynamical change of motor inductance, this paper employs online modification of inductance parameters, according to the flux saturation, then transforms the non-linear equations into linear ones, and so compacts the structure of controller. Thirdly, in view of the maximum torque output is extraordinary requisite when Electrical Vehicle start up, accelerate, and overtake, this paper issues the maximum torque when both inverter and battery capacity is corporeally definite. Fourthly, on-line differentiating and analyzing both rotor's time constants and its flux by using the expanding order reduction and discrete Kalman filter equation are fathomed, in order to realize high-performance trans-vector algorithm, and also the simulation results are presented. Fifthly, the close-loop regenerative brake system, when the EV's torque is set constant, is deduced; at the same time, unification of electric drive model and brake model is executed. Combined with maximum charge current demands, the mathematical model for online yielding torque as a demand.

本论文针对车载异步电机在矢量控制器所遇到的问题：定转子电阻随温度变化及随电机饱和程度变化的转子电感及定子电感而影响电机调速性能提出一套完整的解决方案：分析了电流型逆变器和电压型逆变器在实现矢量控制时控制器参数的计算，分析出对于电流型逆变器在采用定子磁场定向时，其性能同时具有矢量控制和直接转矩控制两者的优势，并对电压型逆变器在转子磁场定向下的模型进行了仿真研究；建立异步电机非线性模型，推导出考虑磁饱和时异步电机在同步旋转坐标系及静止坐标系下的方程，并做出了仿真结果，建立异步电机在非线性模型下的矢量控制调节器的PI参数设计，在实际应用中，若忽略电机电感的动态变化，可以根据当前的磁路饱和状态而在线修正电感参数，从而将非线性方程线性化，降低控制器的复杂度；考虑到电动汽车在起动和加速超车时需要电机有最大转矩输出，本文讨论在逆变器容量一定和电池供电能力有限的情况下电机最大转矩输出问题；推导了异步电机扩展降阶、离散卡尔曼滤波方程在线辨识转子时间常数和转子磁通，用于实现高性能的矢量控制算法，并给出了仿真结果；推导出了电动汽车恒转矩给定的闭环回馈制动系统，实现了电动控制模型和制动模型的统一，而且结合铅酸电池最大充电电流的要求，为制动转矩在线给定建立了数学模型；设计了基于双DSP系统的高性能矢量控制器软硬件框图，并以大量实验数据说明矢量控制在电动汽车应用的实际应用状况。

Acted by taper-face component of centrifugal force together with scroll conveyor,the cake moves interruptedly toward the bi end of the screen-bowl and is discharged out of the screen-bowl,and then out of the machine through a discharging tank.

电机带动转鼓及同心安装的输料螺旋，以一定的差速同向高速旋转，物料由进料管连续引入，均匀分布到转鼓底段滤网壁上，在离心力作用下，液相物穿过滤网和转鼓内形成滤渣，滤渣在离心力的锥面分力和输料螺旋的共同作用下，连续不断地向转鼓大端移动排出转鼓，经卸料槽排出机外。

Locations of transgenes in the recovered plasmids were determined by southern blotting. And then adjacent sequences of transgenes integration sites in three recovered aberrant classes were subcloned and analyzed. Transgene of A-2 was inserted into common carp DNA sequences homologous to the mouse phosphoglycerate kinase-1 gene and those of A-3 and A-6 were inserted into common carp DNA sequences homologous to the human epidermal keratin 14 gene and sequence of common carp β-actin gene, respectively.

通过Southern杂交对转植基因在回收质粒上的位置进行了定位分析，并对三种回收到的变异型转植基因旁侧顺序进行了亚克隆和顺序测定。A-2的转植基因整合到了与小鼠磷酸甘油酸盐激酶-1基因同源的鲤鱼基因组顺序旁侧，A-3的转植基因整合到了与人表皮角蛋白14基因同源的鲤鱼基因组顺序旁侧，A-6的转植基因整合到了普通鲤鱼β-actin基因顺序中。

1,After transfected the mutated mtDNA of colorectal carcinoma,the mtDNA D-loop region of the transfected cells displays new mutation points.2,The external source pieces of the mutated mtDNA can integrate to nuclear genome after transfection.3,There's no differences in apoptosis between combinations after transfected the mutation of mtDNA in NIH3T3 and LST cells.4,The mutated mtDNA may affect the action mechanism of occurrence and development in colorectal carcinoma through affecting its mtDNA mutation or integrating exogenetic mtDNA to its nuclear which may cause the abnormal expression of oncogene or anti-oncogene.

（1）转染突变的大肠癌细胞mtDNA后转染细胞的mtDNA均可发生多处的突变位点。（2）通过转染后突变的外源性的mtDNA可以整合到核基因组内。（3）突变的mtDNA转染LST细胞及NIH3T3细胞后，不影响转染细胞的凋亡改变。（4）mtDNA的突变可能通过影响体细胞mtDNA的突变和通过外源性mtDNA在核内的整合从而影响癌基因或抑癌基因的表达异常，从而参与肿瘤的发生发展。线粒体DNA；D―环区；突变；质粒；pcDNA3.1；转染

The Metonymical property manifests itself in the extension of the nominatum of body terms to the person, to the unit of measurement, to the physical features or functions possessed by body parts, and to the action of body parts.

人体词语词义的转喻性包括人体词语转指人、人体词语转指长度单位、人体词语转指人体相应部位的特征或功能、人体词语转指人体部位相应的动作行为四个方面的转喻。

Based on the empirical studies of arbitrage process and results on domestic convertible bond market, we have drawn the follow conclusions: on domestic convertible bond market, the arbitrage actions conducted by institutional investors help to reduce risk and increase return; meanwhile, arbitrage actions help to effectively price the bond and encourage its bonders to exercise the conversion right.

本文作者通过对国内转债交易市场中投资套利过程及结果的实证分析，提出了研究结论：在国内转债市场中，机构投资者进行的转债套利操作行为有利于机构大资金在进行转债投资实践中回避风险、提高收益；同时，套利行为可以促进转债有效定价并能够推动持债者积极转股等。

To classify metonymies, it is necessary to take into account two criteria, the degree of genericity and the ontological nature of domains, according to which metonymies can be classified into two kinds, high-level metonymies and low-level metonymies.

关于转喻的分类，本文提出两个标准，类属程度和认知域的本体特征。根据这两个标准，转喻可简约地分为高层转喻与低层转喻。高层转喻直接利用类属ICM进行映射，影响语言结构，从这层意义上说，它是语法转喻。

Direct left turn from driveways is considered as a contributor to accident and delay in at-grade intersections. Safety benefits of prohibiting direct left turn from driveways have been identified in many studies. Several engineering alternatives have been developed to accommodate prohibited driveway left turns. These alternatives are (1) right-turn followed by U-turn at the down stream intersection;(2) right-turn followed by median U-turn before the intersection;(3) right-turn followed by median U-turn after the intersection;(4) upstream Jug Handle, and (5) downstream Jug Handle.

鉴于直接左转机动车交通常是路口事故和延误的诱因之一，左转禁驶在许多研究中都作为一项安全措施处理，直接左转处理方式有多种，大致包括：(1)下游路口右转后U形调头直行；(2)当前路口右转后在下一路口前的隔离带打U形调头直行；(3)当前路口右转后在下一路口后的隔离带打U形调头直行；(4)在当前路口上游打&壶柄状&转行；(5)在当前路口下游打&壶柄状&转行。

The transient transfection efficiency was measured by flow cytometry. The stable transfected cells were screened by G418. Results Electrotransfection had the highest transient transfection efficiency (56.8%), with the most masc clones of stably transfection and the least time taken to form big masc clones (20 days). Cationic polymer mediated transient transfection had the lowest efficiency (1.7%), with the least masc clones and the longest time taken to form the big masc clones (30 days). Cationic liposome transfection had a transient transfection efficiency of 39.9% and took 25 days to form the big masc clones.

结果 电转染有最高的瞬时转染效率(56.8%)，稳定转染得到的阳性克隆最多，且从细胞筛选到扩增至得到阳性大克隆所用的时间也最少（约20d）；用阳离子聚合物瞬时转染率低(1.7%)，稳定筛选同样可以得到阳性大克隆，但是数量很少且从细胞筛选到扩增至阳性大克隆所用的时间也最多（约30d）；阳离子脂质体转染效果居中，瞬时转染率为39.9%，从细胞筛选到扩增至得到阳性大克隆所用的时间约25d。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了