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Results The cells transfected with E1AF is remarkably more invasive than those of control group. The image analysis and PCNA expression of the tumor cells demonstrate that transfected cells are m ore actively proliferative than the untransfected cells.

结果 转染后细胞的侵袭能力较转染前明显增强，肿瘤细胞图像分析和PCNA的表达，均显示转染后的肿瘤细胞增殖明显活跃。

Expression of tert and hprt genes were detected simultaneous in all single-copy embryos developed at 4-C (65h-post hCG, 4/4), 8-C (75 h-post hCG, 4/4), morulae (93h-post hCG, 4/4) and blastocyst (118h-post hCG, 4/4) stages and the level of transcription increased.

结果表明，持家基因hprt在成熟卵母细胞受精前后，以及胚胎早期发育过程中均存在转录本。40%卵母细胞中存在的tert mRNA在受精后很快降解，检测不到；胚胎基因组在2-C早期开始转录tert mRNA，转录水平逐渐上升。

Results UCMSCs could be effectively infected by adenovirus containing GFP gene in vitro, the transfection efficiency has the dose-effect relationship with multiplicities of infection.

结果 腺病毒介导的GFP基因对于UCMSCs具有较高的转染效率，转染效率与MOI值具有量效关系，当MOI为100倍时，转染效率达95%。

The effect of transgenx Bt cotton on non-target insects The kinds of the dominant species of pests in transgenic cotton field had obvious change, in which the H. armigera was no longer the main pest and the piercing-sucking pests such as Aphis gossypii, Tetranychus urticae, Thrips tabaci etc. rose the main pests. The kinds of the dominant species of predatory natural enemies seemed to be no variation, which was still Propylaea japonica Thunberg. Because of the effect of transgenic Bt cotton on H. armigera laevae-the development delay, the weight reduction, which affected the qualities of parasitic enemies, leading to the reduction of the weight of cocoon and the weight of adult, and the reduction of H.

转Bt基因棉对节肢动物群落整体结构及时序变化的影响 2001保铃棉与冀抗668的相似系数最高(0.8246)，冀抗668、保铃棉与常规防治的相似系数依次0.6459、0.5829，保铃棉和冀抗668与常规不防治的相似系数为0.5669、0.4485.2002 年与冀杭668的相似系数最高(0 。6887)，保铃棉和冀抗668与常规防治相似系数为0.4739、 0.3828，保铃棉和冀抗668与常规不防治的相似系数为0.3124、0.3999，此结果表明，两转Bt 基因棉田节肢动物群落的整体结构一致，相似性较大，与常规防治相比，相似系数有所降低，表明常规功治棉田中人为控制因素对节肢动物有一定影响；转Bt基因棉对节肢动物群落有一定影响，但并不显著。

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Human fibroblast interferon mRNA was prepared from poly I:C-cycloheximide superinduced human diploid cells by phenol-octanol exhaustion method followed by oligo-cellulose chromatography.

其主要实验根据是：超诱导细胞mRNA含量明显高于正常细胞；干扰素mRNA在非洲鲫鱼卵母细胞内转译的产物具有明显的抗病毒活性；转译时~14C-苯丙氨酸的掺入明显高于正常细胞；种属特异性实验及酶的敏感性实验证明转译产物中存在的抗病毒物质是一种人的干扰素蛋白质。

The steady-state temperature distribution in the rolling tire is predicted with finite element method according to the uncoupling idea.

进行了转鼓试验下滚动轮胎稳态温度场的测试；采用试验与计算相结合的方法验证了轮胎在转鼓试验中的实际滚动状态为自由滚动状态；根据解耦的分析思想，进行了转鼓试验下滚动轮胎稳态温度场的有限元分析。

Please a process ■ The purchase please a style * Purchase payment practice week knot make, each Tuesday is transfer pay day, special circumstances next Thursday consider feeling arrangement transfer payment;* All purchase list is confirm signing by the general manager assistant →finance department the cashier examine →finance the manager examine →general manager approval →cashier payment and notice supplier →the accountancy recheck and statistics;* Cash on delivery from purchase specialist confirm incoming goods amount, on the receipt signature of the supplier confirm payment amount of money, and at purchase list top signature confirm method of payment is cash payment, confirm without any error empress notice cashier payment, the accountancy recheck.

请款流程■采购请款*采购货款实行周结制，每周二为转帐付款日，特殊情况下周四酌情安排转帐付款；*所有采购单由总经理助理确认签字→财务部出纳审核→财务经理审核→总经理批准→出纳付款并通知供应商→会计复核并统计；*货到付款时由采购专员确认来货数量，在供应商的收据上签名确认付款金额，并在采购单上签名确认付款方式为现金支付，确认无误后通知出纳付款，会计复核（区分现金支付与转帐支付，确保不重复付款）。

Fluorescence staining of cell framework and nuclei were conducted, the result of which revealed that the transfected cells displayed disorder in cell skeleton, shrinkage and cleavage in nuclei. It was illuminated that chromosome DNA occurred cleavage and fragment since the result of TUNEL detection was positive. The transfected cells also displayed more typical apoptotic character, including exposure of phosphatidylserine in the plasma membrane by Annexin V staining detection, shrinkage nuclei, chromatin condensation, plasma membrane blebbing and chromatin breakage fragments by electron microscopy analyses.

细胞骨架和细胞核染色结果表明，AIF基因转染细胞呈现细胞骨架的紊乱、细胞核固缩、分裂等变化；TUNEL检测呈阳性，说明有染色体DNA出现断裂；Annexin V染色检测到转染细胞出现胞膜磷脂酰丝氨酸的暴露；电镜观察的结果显示，转染目的基因组细胞呈现细胞膜出泡、细胞核固缩、染色体凝集和断裂等典型的凋亡细胞特征。

In a typical money transfer, we anticipate that a customer located in the United States will conduct money transfer transactions on the internet via our website, which we intend to develop. Utilizing our website, which has yet to be developed, a customer will transfer money using a credit card or a debit from a bank account.

典型的转帐中，我们预期美国的顾客将通过我们的网站管理转帐交易，至今已经得到发展，顾客可以运用一张信用卡从银行存款转帐。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。