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The late phase expression cassette suited for adenovirus vectors was also constructed. The HBV S gene, HBV preS2+S gene and the late phase expression cassette +HBV S genes were respectively cloned into the Ad5 vector downstream of the E3 promoter. Semiconfluent monolayers of 293 cells were cotransfected with the above constructed plasmids and Ad5 DNA EcoRI A fragment. The progeny adenoviruses named rAd5S, rAd5MS, rAd5S2S were harvested.

通过共转染优化实验，建立了脂质体转染腺病毒的有效方法，并构建了适合腺病毒载体的晚期表达盒，进一步将乙型肝炎表面抗原基因、HBV preS2+S、腺病毒晚期表达盒+HBV S基因插入E3区，与EcoRI消化的Ad5 DNA A片段共转染细胞，并获得重组腺病毒，相应命名为：rAd5S、rAd5MS、rAd5S2S。

Transient responses of the tiltrotor during the transition flight are calculated aiming at semispan soft-in-plane tiltrotor models with the elastic wing.

以半展长弹性机其全铰接式倾转旋翼机模型为例，分析倾转旋翼机倾转过渡飞行瞬态响应时间历程。

Take the 10~12ml single-valve capacity as the example, with flings turns the head to separate evenly, 36.000rpm 60 the hour, turns the head with the angle type to separate 45, OOOrpm 36 the hour, the former including adds the deceleration to use in common 130,000,000 revolution of actuation department lives, the latter must use 100,000,000 revolution of actuation department lives, this was 100 ~ 20,000,000,000 transfers to at that time the supercentrifuge total life looked, without doubt each time tests the expense to be excessively high, in addition the CsCl amount used are many, price expensive and so on factors, causes this kind of separation purification work to become the very expensive experiment.

以10~12ml单管容量为例，用甩平转头分离，36.000rpm 60小时，用角式转头分离45，OOOrpm 36小时，前者包括加减速在内共用去1.3亿转驱动部寿命，后者也要用去1亿转驱动部寿命，这对当时超速离心机总寿命为100~200亿转来看，无疑每次实验费用过高，加上CsCl用量多、价格贵等因素，使这类分离纯化工作成为非常昂贵的实验。

NodD binds to and bends target promoters through anchoring two tandem and individual specific DNA sites. NodD functions as a tetramer, which has a V-shaped main body. Tetrameric NodD is to change its own conformation rather than its oligomeric forms in response to small signal molecules. The specific interaction between each NodD DNA-binding domain and each specific DNA site does not alter itself in spite of naringenin induction, and the induced conformational change is transferred from protein to DNA. Only the DNA conformation incited by induced NodD is competent for RNA polymerase to form the transcriptional open complex. It cannot be excluded that NodD may have protein-to-protein contacts with RNA polymerase, and that the NodD conformational change may also directly contribute to the transcriptional open complex formation. However, the NodD conformational change itself cannot serve as the determinant of the transcriptional molecular switch.

通过研究，我们提出了初步的NodD操纵子激活模型：第一，四聚体是NodD蛋白的功能单位，它通过铆钉两个串联的相对独立的DNA靶位点结合被诱导的启动子；第二，小分子配基的结合是改变NodD四聚体的构象而不是引发不同形式的寡聚体，在我们的模型中，NodD四聚体缩小其V形主体的弯折角，进而缩短其DNA结合功能域的间距；第三，小分子信号的诱导并没有改变NodD的DNA结合域和其DNA靶位点的相互作用，NodD的构象改变由蛋白质经其双铆钉位点传递给DNA；第四，只有诱导状态的NodD激发的DNA构象才能有效地使RNA聚合酶形成转录开放复合物；第五，不排除NodD与RNA聚合酶可能有直接的相互接触位点，不排除NodD构象的改变可能直接有利于RNA聚合酶形成转录开放复合物，但是我们认为NodD构象改变本身不是充当转录激活开关的决定因素。

It is the first time to give the formulae of non diagonal matrix elements of tensor operator s which can be employed to describe the ro vibrational interaction for linear tetratomic molecules.

利用Lie代数方法研究了四原子分子振转相互作用，在代数框架内首次给出四原子分子振转相互作用的张量算子非对角矩阵元的表达式，利用这些表达式对线型四原子分子HCCF振转相互作用的l doubling进行了计

LAA mechanical activity prior to ECV is highly related to maintenance of sinus rhythm in NVAF, and LAA "stunning" developed a thrombogenic milieu. Patients with atrial fibrillation should be given full anticoagulation to prevent thrombogenesis in left atrial and LAA.

NVAF患者电转复前左心耳收缩功能与窦性心律的维持高度相关；转复后左心耳&顿抑&，出现了血栓易于形成的条件，故对此类患者电转复后应给予足够的抗凝治疗，预防左房与左心耳血栓形成。

JAK-STAT signal transduction pathway was involved in the pathological processes in the retinal after transection of the optic nerve.

Janus激酶-信号转导子与转录激活子信号转导途径可能通过STAT3蛋白的活化，参与视神经切断后视网膜内的病理改变过程。

At first, it talks about the origin of the animal husbandry and transhumance, and the ethnic process of the formation and development of the Uzbek nationality, with the following the argument of the significance of the animal husbandry in the ethnic process of the Uzbeks. This half of the paper also makes comments on the family history and the history of their transhumance of the Uzbeks of Danangou by dividing the history into several phases.

本文前半部分以乌孜别克族的历史及其游牧生活为中心，首先论述了游牧业与转场的起源和乌孜别克族的民族过程，分析了游牧业在乌孜别克族形成与发展过程中的意义，随后讲述了木垒大南沟乌孜别克族的家族史和转场史，并且对他们的转场历史加以分期进行论述。

S-adenosylmethionine is a naturally occurring molecule, which widely distributes throughout tissues and fluids. It has been one of the very important pharmaceuticals because of its essential role in transmethylation, transsulphuration and transaminopropylation.

S-腺苷蛋氨酸是一种广泛存在于各种组织和细胞中的生物小分子，具有转甲基、转硫和转氨丙基等重要生理作用，已成为治疗肝损伤、胆汁郁积和抑郁症等疾病的重要药物。

Permanent magnet synchronous motor driven by triphase sinusoidal current is research object in this paper. On the basis of analysis of the structure, movement principle and math model of PMSM, Fundamental theories and structure of the system of PMSM alternating current servo systems based on DTC are discussed. Then Simulation models of traditional PMSM DTC and PMSM DTC based on vectors subdivision are established separately.

本文以三相正弦波驱动的永磁同步电机为研究对象，在分析永磁同步电机结构及数学模型的基础上，系统阐述了基于直接转矩控制的永磁同步电机交流伺服系统的基本原理和系统构成，并在MATLAB仿真环境下分别建立了传统直接转矩控制和基于矢量细分算法的直接转矩控制系统的仿真模型。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。