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The invention discloses a software security ensuring method based on program internal behavior monitoring which comprises,(1) the language code analyzer analyzing the program control stream, adding description control stream transformation code, adding assisting protection function for the program, then compiling into performable program,(2) transferring the treated program to a program charging device, determining whether the current program has a protection function,(3) submitting operation system the protection information of the progress and setting the program progress protection sign to TURE,(4) the operation system kernel determining the progress protection sign, go to step 7 if the result is FALSE,(5) the operation system kernel examining the progress control stream conversion consistency,(6) continuing the progress,(7) finishing.

本发明公开了一种基于程序内部行为监控的软件安全保障的方法，其步骤是：1。语言代码分析器分析程序的控制流，并添加描述控制流转换代码，添加程序的协助保护功能，然后编译成可执行程序；2。处理过的程序递交给程序加载器，判断当前程序是否具有保护功能，如果有则加载适当的运行环境，否则转到4；3。向操作系统递交该进程的保护信息并设置该程序进程的保护标志为真；4。操作系统内核一旦获得控制权利，则判断进程的保护标志，如果为假转到7；5。操作系统内核检查该进程控制流转换的一致性，如果不一致，发出安全警告，并杀死进程，转到7；6。继续运行进程；7。结束。本发明的显著优点是提高了安全保障方法的精确性和通用性。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

Methods Routine immunization of BALB/ c mice by a tansfected cell line 293T/ B7-H4 as antigens.Then the immunized spleen was fused with SP2/0,a kind of plasmocytoma cells, and screen the positive clones by FCS with the 293T/B7-H4 as a positive control while 293T/ mock as the negative control.

以高表达人B7-H4分子的基因转染细胞株293T/B7-H4为免疫原，常规免疫BALB/c小鼠，采用B淋巴细胞杂交瘤技术进行细胞融合，并以该基因转染细胞293T/B7-H4作为阳性筛选细胞，以转空质粒的对照细胞293T/Mock作为阴性对照细胞。

By using liposome-mediated gene transfection technology, plasmid pCMVSV40T/PUR containing SV40Tag was transfected in primary embryonic precartilaginous stem cells, while non-transfected cells served as negative controls.

利用脂质体介导基因转染技术将含有SV40Tag的质粒pCMVSV40T/PUR转染原代胚胎前软骨干细胞，以未转染细胞作阴性对照。

In Smad pathway, by using cyclohexamide and proteosome inhibitor MG132, we showed that BMP-2 significantly increased inhibitory Smad ( i.e., Smad7) and down-regulated the half-life and protein levels of TbetaRI by poly-ubiquitination and proteosomal degradation.

在 Smad 讯息路径中，藉由转译抑制剂，cycloheximide 以及 proteosome 抑制剂，MG-132的刺激，我们发现第二型骨头成形蛋白可显著增加抑制型 Smad：Smad7的表现，使第一型乙型转形生长因子接受器走向 ubiquitin-proteosome 的水解路径，进而降低第一型乙型转形生长因子接受器的半衰期及蛋白质表现。

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以下为引用的内容：致内容分享、点播业务站长：近日，联盟合作站点因版权问题与版权方存在一些潜在的矛盾，为更好的帮助点播站长归避版权风险，降低运营风险，经联盟多次商讨决定，对点播业务及内容分享业务做出如下调整：1、点播门户里涉及内容提供商的影片，跳转到内容提供商的域名进行播放；例如，用户点击播放影片《武林外传》的时候，播放器就会向服务器查询到《武林外传》的提供商捷报，得到查询结果之后播放器自动将页面跳转到 http：//xunlei.jeboo.com 这个捷报域名下面一个播放页进行播放，大大减少了播放影片的版权风险；但是为了安全，该播放页不含播放器之外的任何内容。2、使用联盟播放器进行播放的时候，跳转到内容提供商的域名进行播放，具体播放机制同上；3、关闭点点播的点播服务，且不可再使用点点播进行影片发布和影片播放，关闭时间约在8月底，请相关的站长尽快更改网站使用的技术和编辑自己网站的内容。4、停止新建看看频道页，原有合作站点业务将于8月底正式关闭；5、8月底停止内容分享的内容更新，9月底正式关闭内容分享业务；推荐此类合作站点选择联盟的点播门户产品，让用户直接享受稳定的在线播放。

All the transfected and un-transfected rMSCs were attached to Allo-DBMs. These new biomaterials were implanted in muscle bags and segmental radius defects of the New Zealand white rabbits, and some controlled material groups were established for comparison. All the biomaterials and the controlled materials were assessed by gross observation, radiographical and histological methods.

取兔自体骨髓基质细胞培养扩增，用脂质体介导的方法转染人骨发生蛋白2基因，将转染和未转染人骨发生蛋白2基因的自体骨髓基质细胞附和于异体兔脱钙骨基质形成新的生物植骨材料，连同空白及单纯脱钙骨基质对照组植入兔前肢肌袋和桡骨缺损。2、4、6、8周分别取材进行大体，放射线和病理观察。

Postoperative right-sided obstruction occurs more frequently after ASO repair of Taussig-Bing heart than after TGA arterial switching, leading to higher reintervention rate.

Taussig-Bing 心脏畸形行心脏转位术（ASO比大动脉转位行心脏转位术后更可能发生术后右室流出道梗阻，导致更高的再介入率。

The replicons were detected and characterized by RT-FUR. IFA. Renilla Luciferase assay system and real time RT-FUR. respectively. Results It was shown that SLB2 mutant did out significantly affect the translation of the input RNA.

将突变体（包括相关的阳性和阴性对照复制子）分别线性化并体外转录成RNA后，取等量各转录体RNA，以脂质体法分别转染BHK-21细胞。

Softly wipe the surface of printing film using tissue from one end to the other end of objects, for piling out water (Don't strongly wipe for protecting the printing film from staving or ruckling).

用纸巾轻轻擦转印膜的表面，将水份挤出，（不可过于用力，以免将转印膜弄破或弄皱），擦转印膜时可从物品的一边慢慢擦向另一边，一次不行可多擦几次，并可细微调整图像位置，直至把物品水份完全挤干。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。