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Adult rats are divided into control group, intrapericardial injection group and negative group, sublingual vein injection group and negative group. 6ds after transfection, issues of heart, liver, lung and kidney are stained with X-gal to observe transfection to myocardium and non-target transfection. 3. CaN Aβ gene silencing in vivo. Adult rats are divided into Control group, hypertrophy model group, intrapericardial injection group and negative group, sublingual vein injection group and negative group.
二整体动物水平上探讨心肌有效转染途径:以PLacZ为报告基因,将成年大鼠分为空白对照纽(生理盐水心包腔内注射后超声导入);转染质粒心包腔内注射组及其阴性对照组:质粒+微泡+酶的混合物心包腔内注射,超声导入;转染质粒舌下静脉注射组及其阴性对照组:质粒+微泡混合液经舌下静脉注射,超声导入。
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Finally, we also examined the diferentiation efect of Ad-GFP on myoblasts.3.Study of the transfection of Ad-GFP to myoblasts which Transplanted in the intrarectal of the immune defect rat.The immune defect rats Were randomized in to 2 groups( 10 in each group):The experimental group transplanted with myoblasts transfected by Ad-GFP; the control group injected PBS.
并观察转染了Ad-GFP后是否会对成肌细胞的分化融合有影响。3、转染Ad-GFP的成肌细胞在免疫缺陷小鼠直肠内移植的实验研究将20只免疫缺陷小鼠随机分成2组(每组10只),移植组肛管直肠粘膜下层注射转染Ad-GFP的成人成肌细胞,对照组注射PBS。
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The exploration of the metaleptic reality, of the Platonic metaxy, as well as the articulation of the exploratory action through language symbols, in Plato's case of his Myths, are the central concern of the philosopher's efforts.
这种由言辞开启的对现实世界的探索,(柏拉图式的言辞转喻,译注:&言辞转喻的实在&即所谓的idea,即&理念&,而言辞转喻即理念的辨证运动,此后都翻译为&理念&),也可以称为一种借助语言符号而达到对世界清晰理解的探索行为(表现在柏拉图的虚构对话中),关乎着哲学家最核心的努力。
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Based on the classification of metonymy, this paper has compared and analyzed the similarity and difference of metonymic thought between Chinese and English respectively from lexical, grammatical and discoursal perspectives.
本文以转喻的分类为基础,分析了汉英在词汇,语法,话语三个层次上转喻思维的异同,从而在揭示一般思维方式和语言能力之间关系的同时,进一步指出转喻研究在二语习得中的教学意义。
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Polysemy is discussed from the perspective of prototype, cognitive metaphor and metonymy, discovering that a polysemic word constitutes a semantic category with its primary meaning as the prototype while other members in that category are derived from it, exhibiting a family resemblance and degrees of typicality as well, and that the extension of word meaning is mainly achieved through metonymical thinking based upon contiguity and metaphorical thinking based upon similarity.
利用原型范畴理论、认知隐喻和转喻角度分析一词多义现象,认为一个词的多个义项是从原型义项演变而来的,并且表现出不同程度的家族相似性,同时又表现出不同程度的原型义项身份;词义延伸又主要通过转喻和隐喻的认知思维结构,即转喻的邻近性和隐喻的相似性思维特征来实现。
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More importantly, with this empirical investigation we manifest how constantly human beings think and speak metonymically.
藉由探究转喻在中文真实口语语料中的呈现,我们证实了转喻系统的确不断地在语言和思想中运作,换言之,转喻是我们思考和说话所仰赖的重要机制。
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But the effluent ammonium in the anoxic reactor, where enough NO2 were present, was equal to the blank system, and no ammonium was converted to such nitrogen compounds as NO2- and N2 by Nitrosomonas eutropha using NO2 as electron acceptor, which maybe caused by lack of the function bacteria. There were two ANAMMOX reaction pathways in the one-stage autotrophic nitrogen removal system. One way was that after part of NH4+ was oxidized to NH2OH under aerobic conditions, NH2OH and NO2- were converted to N2O under anaerobic conditions, at last N2O was further converted to N2 which realized the nitrogen removal; Another way was that at first NO2- was reduced to NH2OH, NH2OH reacted with NH4+ to form N2H4, which was further converted to N2 subsequently, realizing the nitrogen removal.
结果表明:单级自养脱氮系统内6.72%的氨氮是通过吹脱等物化作用去除的,不超过6.02%的氨氮是通过传统硝化反硝化途径去除的,87.26%左右的氨氮是由自养脱氮途径去除的,自养脱氮反应起主要脱氮作用;在足够NO2存在且缺氧的条件下,单级自养脱氮系统内的出水氨氮浓度与空白反应器相当,NH4+并没有被亚硝化单胞菌以NO2为电子受体氧化为NO2-和N2等化合物而得以去除,可能是因为系统内不存在该代谢功能的亚硝化功能菌;单级自养脱氮系统内存在两条ANAMMOX反应途径:其中一条途径即NH4+在好氧条件下被氧化为NH2OH后,生成的NH2OH与系统内的NO2-在缺氧条件下被转化为N2O,N2O则进一步被转化为N2而实现氮的去除;另外一条途径即NO2-首先被还原为NH2OH,生成的NH2OH则与系统内的NH4+反应生成N2H4,N2H4继续被转化为N2而实现氮的去除。
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While AcMNPV and BmNPV had no stimulatory effect in nonsensitive cell lines BmNPV hr3 has been demonstrated to increase the transcriptional activity of cislinked hel510 promoter. In this study, Sf-21, Bm-N and Hi-5 cell lines were transfected with pBmhel510luc-hr3down, and then were infected with each of AcMNPV, BmNPV and HyNPV. The results indicated that viral factors could interact with hr3 to significantly copromote the transcriptional level of hel510 promoter.
BmNPV hr3已被证明具有增强子功能,将hr3与hel510启动子顺式连接的质粒pBmhel510luc-hr3down转染Sf-21、Bm-N和Hi-5细胞后,再用上述三种病毒分别感染每一种细胞,hel510启动子的转录活性得到显著提高,表明病毒因子能够识别hr3,二者通过相互作用,共同增强解旋酶基因启动子的转录水平。
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Coding and non-coding sequences are expected to have very different patterns of sequence changes because of the following characteristics of coding sequences: biased codon composition, higher conservation, absence of stop codons and frameshifts, higher fraction of synonymous than nonsynonymous substitutions, higher frequency of substitutions corresponding to changes of chemically similar amino acids, higher proportion of mutable contexts.
我们可以预期可转录的序列及不可转录的序列会有相当不同的序列改变特徵,因为可转录的序列有以下特色:偏颇的密码子组成,较高的保存度,有终止密码子和移码的出现,相较於异义取代有较高比例的同义取代,较高的频率出现化学结构相似的胺基酸取代,较高比例的可变之前后序列。
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Methods The 201 cases in 1979~1996 were retrospectively summarized. The clinical features including chief complaints, sex distribution, age at first visit, laterality, type of presentation, ocular deviation in the primary position, refractive errors, amblyopia, globe retraction, change of the palpebral fissure, upshoot and downshoot in adduction, binocular single vision, and its associated ocular and non-ocular anomalies were analyzed.
自1979~1996年回顾性地总结了201例DRS,对它的临床特征,包括主诉、性别分布、初诊年龄、侧向状态、表现类型、原在位的眼位偏斜、屈光不正、弱视、眼球后退、睑裂改变、内转时眼球急速上转和下转、双眼单视及其伴随的眼部和非眼部异常等予以分析。
- 推荐网络例句
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But we don't care about Battlegrounds.
但我们并不在乎沙场中的显露。
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Ah! don't mention it, the butcher's shop is a horror.
啊!不用提了。提到肉,真是糟透了。
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Tristan, I have nowhere to send this letter and no reason to believe you wish to receive it.
Tristan ,我不知道把这信寄到哪里,也不知道你是否想收到它。